African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12257

Full Length Research Paper

Genetic variation via SSR polymorphic information content and ecological distribution of Nigerian sesame

Olusola Thomas ODUOYE
  • Olusola Thomas ODUOYE
  • National Centre for Genetic Resources and Biotechnology (NACGRAB), PMB 5382, Moor Plantation, Ibadan, Oyo State, Nigeria.
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Opeyemi Ayokunnu OLUWASANYA
  • Opeyemi Ayokunnu OLUWASANYA
  • National Centre for Genetic Resources and Biotechnology (NACGRAB), PMB 5382, Moor Plantation, Ibadan, Oyo State, Nigeria.
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Oluwabukola Omowumi ARIKAWE
  • Oluwabukola Omowumi ARIKAWE
  • National Centre for Genetic Resources and Biotechnology (NACGRAB), PMB 5382, Moor Plantation, Ibadan, Oyo State, Nigeria.
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Abiodun SUNDAY
  • Abiodun SUNDAY
  • National Centre for Genetic Resources and Biotechnology (NACGRAB), PMB 5382, Moor Plantation, Ibadan, Oyo State, Nigeria.
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Omowumi Abidemi AYEKUN
  • Omowumi Abidemi AYEKUN
  • National Centre for Genetic Resources and Biotechnology (NACGRAB), PMB 5382, Moor Plantation, Ibadan, Oyo State, Nigeria.
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Oyejide Suara OYENPEMI
  • Oyejide Suara OYENPEMI
  • National Centre for Genetic Resources and Biotechnology (NACGRAB), PMB 5382, Moor Plantation, Ibadan, Oyo State, Nigeria.c Resources and Biotechnology (NACGRAB), PMB 5382, Moor Plantation, Ibadan, Oyo State, Nigeria.
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Jandong, E. A.
  • Jandong, E. A.
  • Department of Agronomy, Taraba State University, P. M. B. 1167, Jalingo, Nigeria.
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Uguru, M. I.
  • Uguru, M. I.
  • Department of Crop Science, University of Nigeria, Nsukka, Nigeria.
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Okechukwu, E. C.
  • Okechukwu, E. C.
  • Department of Crop Science, University of Nigeria, Nsukka, Nigeria.
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  •  Received: 07 October 2019
  •  Accepted: 10 March 2020
  •  Published: 30 April 2020

Abstract

Sesame (Sesamum indicum L.) of the family Pedaliaceae is an important and old oil crop that is cultivated mainly in the tropical and subtropical regions of Asia and Africa for several economic values. Several molecular markers have been employed to study the genetic diversity of this important crop. The study focused on the genetic diversity through polymorphism information content (PIC) by the use of Simple Sequence Repeat markers among randomly collected 22 Sesame germplasm across 2 ecological zones stored in NACGRAB seed Genebank, Nigeria. The extraction procedure followed Cetyltrimethylammonium Bromide (CTAB) and the recovered DNA were good with average concentration of 337.00 ng/µL and average quality of 1.795. 30 primers were designed but only 12 with highest genome coverage were used to analyzed the genetic data with NTSYS pc ver.2.02 and Power Marker ver.3.5. The PIC ranged between 0.36 in Primer OTO2 and 0.76 in Primer OTO5. The evolutionary relationship was constructed based on the polymorphic primers and according to their ecological locations. The populations were divided to 2 major clads. Of the 2 ecological zones, the more diverse ecological zone is the derived Savana with 13 accessions, while the less diverse is the humid forest with 9 accessions.
 
Key words: Sesame, genetic diversity, polymorphism information content (PIC), ecological distribution, microsatellite.