African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Full Length Research Paper

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Ricardo Ernesto Bianchetti
  • Ricardo Ernesto Bianchetti
  • Laboratory of Plant Physiology, Department of Botany, Institute of Biological Sciences, Federal University of Juiz de Fora (UFJF), CEP 36036-900, Juiz de Fora, MG, Brazil.
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Cristiano Ferrara de Resende*
  • Cristiano Ferrara de Resende*
  • Laboratory of Plant Physiology, Department of Botany, Institute of Biological Sciences, Federal University of Juiz de Fora (UFJF), CEP 36036-900, Juiz de Fora, MG, Brazil.
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Vinicius Sacramento Pacheco
  • Vinicius Sacramento Pacheco
  • Laboratory of Plant Physiology, Department of Botany, Institute of Biological Sciences, Federal University of Juiz de Fora (UFJF), CEP 36036-900, Juiz de Fora, MG, Brazil.
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Felipe Floriano Dornellas
  • Felipe Floriano Dornellas
  • Laboratory of Plant Physiology, Department of Botany, Institute of Biological Sciences, Federal University of Juiz de Fora (UFJF), CEP 36036-900, Juiz de Fora, MG, Brazil.
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Aline Mystica Silva de Oliveira
  • Aline Mystica Silva de Oliveira
  • Laboratory of Plant Physiology, Department of Botany, Institute of Biological Sciences, Federal University of Juiz de Fora (UFJF), CEP 36036-900, Juiz de Fora, MG, Brazil.
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Jober Conde Evangelista Freitas
  • Jober Conde Evangelista Freitas
  • Laboratory of Plant Physiology, Department of Botany, Institute of Biological Sciences, Federal University of Juiz de Fora (UFJF), CEP 36036-900, Juiz de Fora, MG, Brazil.
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Paulo Henrique Pereira Peixoto
  • Paulo Henrique Pereira Peixoto
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  •  Received: 14 December 2016
  •  Accepted: 10 February 2017
  •  Published: 01 March 2017

Abstract

This work aimed to develop a protocol for the in vitro establishment, multiplication, rooting and ex vitro acclimatization of Mimosa pudica L., a species used in folk medicine and with pharmacological activity. Aseptic cultures were established from seeds inoculated in MS medium, without growth regulators, followed by an in vitro stabilization phase in culture medium supplemented with 2.22 μM BAP. The cultures were transferred to MS medium supplemented with different cytokinins, combined or not with an auxin, aiming its large-scale propagation. The culture medium supplemented with 5 µM BAP plus 0.5 µM NAA provided the highest multiplication rate and top quality plantlets. The combination of 0.6 µM TDZ plus 0.05 µM NAA resulted in higher multiplication rates than in response to combination of BAP plus NAA, although the subsequent maintenance of the cultures in a medium without growth regulators has resulted in low regenerative response. In vitro rooting of micro-cuttings was high even in the absence of auxins. Over 90% of plantlets transferred to the greenhouse survived after the acclimatization phase. Acclimatized plants presented normal vegetative and reproductive development. The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies.

Key words: Biodiversity conservation, ex vitro acclimatization, in vitro rooting, micropropagation, Mimosa pudica.