African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

An antioxidant peptide produced by autolysis reactions from wheat germ

Liming Hu1,2, Ruixue Song2 and Zhenxin Gu1*    
1College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P. R. China. 2Xuzhou Air Force College, Xuzhou, Jiangsu 221006, P. R. China.    
Email: [email protected]

  •  Accepted: 17 November 2011
  •  Published: 21 February 2012

Abstract

Wheat germ was incubated in a buffer solution of sodium citrate and dibasic sodium phosphate and its proteins were hydrolyzed by endogenous proteases to produce peptides. A peptide with high antioxidant activity was purified using ultrafiltration, Sephadex G-25 gel filtration column and consecutive chromatographic methods. The purified peptide was identified as Val-His-His-His (520.66 m/z) using reversed-phasehigh performance liquid chromatography (RP-HPLC) connected online to an electrospray ionization mass spectrometry. This antioxidant peptide showed high reducing power per unit, hydroxyl radical scavenging ratio and Cu2+ chelating ability. This study provides an economical and convenient method to isolate bioactive peptides from wheat germ.

 

Key words: Antioxidant peptide, wheat germ, autolysis.

Abbreviation

 

Abbreviation: RT-HPLC, Reversed-phase high performance liquid chromatography.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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