African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Development of polyclonal antibodies for the detection of recombinant human erythropoietin

Collares Thaís Farias1, Monte Leonardo Garcia1, Campos Vinicius Farias2, Seixas Fabiana Kömmiling3, Collares Tiago Veiras2, Dellagostin Odir4 and Hartleben Cláudia Pinho1*
1Laboratório de Imunodiagnóstico, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Brazil, 96010-900, Pelotas, RS, - P.O. Box 354, Brazil. 2Laboratório de Embriologia Molecular e Transgênese, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Brazil, 96010-900, Pelotas, RS, - P.O. Box 354, Brazil. 3Laboratório de Genômica Funcional, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Brazil, 96010-900, Pelotas, RS, - P.O. Box 354, Brazil. 4Laboratório de Vacinologia, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Brazil, 96010-900, Pelotas, RS, - P.O. Box 354, Brazil
Email: [email protected]

  •  Accepted: 26 August 2013
  •  Published: 11 September 2013

Abstract

Recombinant human erythropoietin (rHuEPO) is detected by using direct pharmacological assays and indirect haematological assays. However, both methods have several limitations including technical challenges and cost-related issues. The aim of this study was to develop polyclonal antibodies against rHuEPO (anti-rHuEPO pAb) that can be used in immunoassays. In this study, we purified anti-rHuEPO pAb that could be used in immunoblotting assays to efficiently detect rHuEPO. Furthermore, these anti-rHuEPO pAb which could also detect rHuEPO that was expressed in a eukaryotic expression system (CHO cells). Thus, the anti-rHuEPO pAb developed in this study may be useful for rHuEPO detection.

 

Key words: Antibodies, rHuEPO, immunoassays, pAb.

Abbreviation

Abbreviations: EPO, Erythropoietin; rHuEPO, human recombinant erythropoietin;CHO, Chinese hamster ovary; ELISA, enzyme linked immuno sorbent assay; SDS-PAGE, sodium dodecyl sulfate poly-acrylamide gel electrophoresis.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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