African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

An efficient system for in vitro multiplication of Ocimum basilicum through node culture

A. Shahzad
  • A. Shahzad
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh-202 002, India
  • Search for this author on:
  • Google Scholar
M. Faisal
  • M. Faisal
  • Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451,
  • Search for this author on:
  • Google Scholar
N. Ahmad
  • N. Ahmad
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh-202 002, India
  • Search for this author on:
  • Google Scholar
M. Anis
  • M. Anis
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh-202 002, India
  • Search for this author on:
  • Google Scholar
A. Alatar
  • A. Alatar
  • Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451,
  • Search for this author on:
  • Google Scholar
Alwathnani A. Hend
  • Alwathnani A. Hend
  • Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451,
  • Search for this author on:
  • Google Scholar


  •  Accepted: 14 February 2012
  •  Published: 15 March 2012

Abstract

 

An efficient in vitro micropropagation system was developed for direct shoot growth ofOcimum basilicum, an important medicinal plant, using nodal explants. The excised nodes were cultured on Murashige and Skoog (MS) medium containing two plant growth regulators (6-benzyladenine and 2-isopentanyl adenine) with various combinations and concentrations for the study of shoot induction. Addition of L-glutamine was essential to induce sprouting of axillary buds. The best condition for shoot growth was with 6-benzyladenine (BA) 10.0 µM + L-glutamine 30 mg/L in MS medium. The optimum shoot formation frequency was 100% and about 13.4 ± 1.80 shoots were obtained from each explant after 8 weeks of culture. Shoots (more than 4 cm) were rooted most effectively in 5.0 µM indole-3-butyric acid (IBA) supplemented with half-strength MS medium. The plantlets thus obtained hardened off and were transferred to natural soil, where they grew well and attained sexual maturity.

 

Key words: Axillary shoot sprouting, L-glutamine, medicinal plant, micropropagation,Ocimum basilicum.

Abbreviation

BA, 6-Benzyladenine; 2-ip, 2-isopentanyl adenine; IBA, indol-3-butyric acid; MS, Murashige and Skoog’s medium (1962); PGRs, plant growth regulator

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 11 No. 22
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES