African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Lectin receptor kinase LecRK-b2 localizes to plasma membrane and functions as a homdimer

  Keqin Deng, Xiushan Li, Dashi Yu, Qiming Wang, Jianxin Zeng, Xiaoying Zhao, Dongying Tang and Xuanming Liu*        
Bioenergy and Biomaterial Research Center, Bioscience and Biotechnology Institute, Hunan University, Changsha 410082, China.
Email: [email protected]

  •  Accepted: 10 July 2009
  •  Published: 20 July 2009

Abstract

 

An Arabidopsis putative lectin receptor-like serine/threonine kinase gene, LecRK-b2, has been characterized. Confocal microscopy images showed that the LecRK-b2-GFP fusion protein is localized to plasma membrane. The results of yeast 2 hybrid showed that lectin domain of LecRK-b2 had self-interaction, while the kinase domain did not work. Split luciferase complementation assays confirmed the yeast two hybrid results and suggested that lectin domain was responsible for the dimerization of LecRK-b2 protein in plant. Furthermore, the recombinant LecRK-b2 protein exhibited autophosphorylation activity.

 

Key words: Lectin receptor kinases, subcellular localization, homodimer.

Abbreviation

Abbreviations: LecRK, Lectin receptor kinase; RLK, receptor like kinase; GFP, green fluorescent protein; LUC, luciferase; EDTA, ethylenediaminetetraacetic acid.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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