African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

In vitro mass multiplication of pomegranate from cotyledonary nodal explants cv. Ganesh

Pushpraj Singh1*, R. M Patel1 and Smita Kadam2
1Aspee College of Horticulture and Forestry, Navsari Agricultural University, Navsari, Gujarat 396 450, India. 2Directorate of Onion and Garlic Research Rajgurunagar, Maharashtra, India.  
Email: [email protected]

  •  Accepted: 22 April 2013
  •  Published: 15 May 2013


For surface sterilization of explants, treatment involving HgCl2­ (0.1 %) for 3 min gave better sterilization of cotyledonary nodal explants. The maximum percentage establishment of cotyledonary node explants was observed on Murashige and Skoog (MS) medium + 1.0 mg/l 6-benzylaminopurine (BAP) + 0.5 mg/l naphthaleneacetic acid (NAA). However, MS medium fortified with 1.0 mg/l BAP + 1.0 mg/l kinetin + 200 mg/l activated charcoal exhibited maximum multiplication rate for the first two subcultures. The maximum frequency of multiple shoots in cotyledonary explants (86.33 %) was observed on treatment MS + 1.0 mg/l BAP + 1.0 mg/l kinetin + 200 mg/l activated charcoal. In vitro rooting of regenerated shoot was found in half strength MS medium supplemented with 0.5 mg/l NAA + 200 mg/l activated charcoal, which recorded the maximum number of root/shoot (4.17) and root length (3.87 cm).In vitro grown plantlets having 5 to 6 cm length of shoot were transferred to vermicompost + soil (1:1v/v) media kept in net house, which showed better survival of plantlet (85.50%) within 11.75 days.


Key words: Establishment, multiplication, rooting, acclimatization, Punica granatum.


MS, Murashige and Skoog medium; B5, Gamborg medium; WPM,Woody plant medium; NAA, naphthaleneacetic acid; BAP, 6-benzylaminopurine; IAA,indole-3-acetic acid; IBA, indole-3-butyric acid; Kin, kinetin; AC, activated charcoal.