African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

In vitro activity of certain antimicrobial agents in combination with Augouardia letestii hexane extracts against methicillin-resistant Staphylococcus aureus

rice W. Obiang-Obounou1, Jang-Gi Choi1, Young-Seob Lee1, Ok-Hwa Kang1,3, You-Chang Oh1, Joon-Ho Keum1, Lamidi Maroufath2, Jung-Rae Rho3, Dong-Won Shin4 and Dong-Yeul Kwon1*  
1College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Jeonbuk 570-749, Republic of Korea. 2Institute of Traditional Pharmacopoeia and Medicine (IPHAMETRA), National Center of Research and Technology (CENAREST), PO Box 1156, Libreville, Republic of Gabon. 3Department of Oceanography, Kunsan National University, Kunsan, 573-701, Republic of Korea. 4Department of Oriental Medicine Resources, Sunchon National University, Jeonnam 540-742, Republic of Korea.
Email: [email protected]

  •  Accepted: 07 September 2011
  •  Published: 24 October 2011

Abstract

The ability of methicillin-resistant Staphycoccus aureus to acquire resistance to most antibiotics is a worldwide concern that necessitated the study of the antimicrobial activity of the medicinal plant Augouardia lestestii alone and in combination with existing antibiotics. The minimum inhibitory concentration was determined by broth microdilution method and the evaluation of synergy was determined by the checkerboard dilution test. In this study, the antimicrobial activity of A. lestestii leaves, stem barks and roots were investigated against methicillin resistant S. aureus strains. The A. lestestii hexane fraction showed good antibacterial activity against all strains with minimum inhibitory concentration ranging from 125 to 250 μg/ml. Further, synergistic effects were observed for all antibiotics (ampicillin, norfloxacin, ciprofloxacin and erythromycin) except for nisin. The study also validated the traditional use of A. lestestii against infectious diseases.

 

Key words: Augouardia lestestii, antimicrobial activity, synergistic combinations, drug-resistance.

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