African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Establishment of Eucalyptus grandis W. Hill ex Maiden in vitro using commercial products for seed treatment

  Moacir Ribeiro Neto1*, Cíntia de Oliveira Martendal2, Flávia Dionísio Pereira1, Edson Luiz Souchie1 and Fabiano Guimarães Silva1  
  1Instituto Federal Goiano – Câmpus Rio Verde, GO. Rod. Sul Goiana, Km 01, Cx. P. 66, Cep. 75901-970, Rio Verde, Goiás, Brasil. 2Universidade Estadual de Maringá, Centro de Ciências Biológicas. Av. Colombo, 5700. Câmpus Universitário, Cep. 87020-900, Maringá, Paraná, Brasil.
Email: [email protected]

  •  Published: 19 June 2013

Abstract

 

Given the current demand for timber from forest species, there is a need to develop new strategies for the mass propagation of eucalyptus. Therefore, this study aimed to determine the effect of different doses of Standak Top® and CoMo Raiz® on the establishment of Eucalyptus grandis W. Hill ex Maiden from seeds in vitroThe experimental design was completely randomized in a 4 × 3 + 1 factorial arrangement(four concentrations: 12, 3, and mL L-1 of the commercial products Standak Top®, CoMo Raiz®, a mixture of both, and a control treatmentwith 20 replicates. The following characteristics were evaluated: Contamination level, shoot length, and the number of leaves. No contamination was observed for the different doses of Standak Top®. Additions of 1 to 3 mL L-1 of Standak Top® to the culture media yielded the greatest shoot length, and the additions of 2 to 3 mL L-1 yielded the greatest number of leaves. Mixing Standak Top® and CoMo Raiz® did not significantly enhance the measured characteristics.

 

Key words: Standak Top®, CoMo Raiz®, tissue culture, asepsis, culture media.