This experiment was aimed to detect garlic infecting virus for three improved garlic varieties (Bishoftu Nech, Tseday and Kuriftu) collected from different garlic production sites of Ethiopia. Garlic cloves were planted in screen house at Biosciences Eastern and Central Africa-International Livestock Research Institute Hub Nairobi, Kenya in 2014. Reverse Transcription polymerase chain reaction (*RT-PCR) technique was applied using two general Potyvirus detecting primer sets designated as CP and Nib. The primers were designed based on deep sequencing information previously generated from infected Ethiopian garlic samples. The RT-PCR diagnosis showed that the three improved garlic varieties were completely infected by potyvirus across all tested sites. Thus, to recover the cultivars two techniques viz. meristem culture alone and meristem culture associated with thermotherapy (cloves treated with 38°C for 60 days) were compared. Indexing by RT-PCR indicated that 77 and 82% in vitro plantlets were found to be virus free from meristem culture alone and thermotherapy associated with meristem culture, respectively. Finally, the information generated from this experiment might be considered to establish virus free garlic dissemination scheme in Ethiopia.

Keywords: Potyvirus, CP-Coat protein, degenerate primer, Nib-Nuclear inclusion protein, reverse transcription polymerase chain reaction.