Development of the multiple antibiotic resistance is the major cause of clinical infections world wide and has been increasing. This study determined the pattern of bacterial multiple antibiotic resistance in hospital sewage samples obtained from Marathwada region of India. The present study had used 48 isolates of bacteria obtained from 6 different locations of Aurangabad, Marathwada India. Antibiotic sensitivity test had been carried out by using disc diffusion method given by Kirby-Bauer and results had been interpreted from zone of inhibitions using CLSI [Clinical Laboratory Standard Institute]. Among all the antibiotics tested, highest level of resistance was observed for beta lactum class [85%], then for Tetracycline 58%, Cefalosporin [58%], quinolones [52%] and gentamycin [45%] [Fig 1]. E coli and Klebsiella pneumoniae are the most prevalent bacteria showing antibiotic resistance to all tested antibiotics with MAR index 1. This study provided baseline data on antibiotic resistance of Hospital sewage samples from Aurangabad city, Marathwada India, which indicates hospital sewage water could be the reservoir of antibiotic resistant bacteria which may further contaminate the drinking water bodies, potentially presenting a public health risk.

Keywords: Antibiotics, Resistance, Multiple Antibiotic resistance, Klebsiella pneumoniae, Escherichia coli, Pseudomonas species, Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogens.