African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Article in Press

Isolation of a novel strain Bacillus sp.in producing neutral and heat stable beta-1,4 endoglucanase activity

Rahman Laibi Chelab， Dan Dan Wang and Xingyong Yang

Neutral a β-1-4 - endoglucanase was produced by aerobic bacteria a newly strain Bacillus sp .RL. Isolated from soil, Chong Qing province, China. In the present study the bacteria produce a significant amount of enzymes after 72 hrs of fermentation CMC medium were 0.1852 U/ml of endoglucanase, 0.5814 U/ml of FPase activity and 2.9940 mg/ml of extracellular protein. These strains showed heavy growth and liquefaction of 7.0, 35°C. The endoglucanase retained 85.5% and 66.9% of original activity at 90°C and pH 10, respectively. The endoglucanase activity was inhibited by CuSO4.4H2O (54%), while the best sulfate source improved enzyme activityFeSO4.7H2O (275%). FPase was markedly activated by CuSO4.4H2O> ZnSO4.2H2O> FeSO4.7H2O> Na2SO3, 180%，158%，152% and119% respectively.

Keywords: Bacillus sp.RL1 strain. Endoglucanase. FPase. Enzymatic assay.　

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