African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Optimization of PCR protocol for ISSR marker based genetic diversity assessment of acid lime [Citrus aurantifolia (Christm.) Swingle] germplasm in Eastern Nepal

Nabin Narayan Munankarmi
  • Nabin Narayan Munankarmi
  • Central Department of Biotechnology, Tribhuvan University (TU), Kirtipur, Kathmandu, Nepal.
  • Google Scholar
Neesha Rana
  • Neesha Rana
  • Molecular Biotechnology Unit, Faculty of Science, Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal.
  • Google Scholar
Tribikram Bhattarai
  • Tribikram Bhattarai
  • Central Department of Biotechnology, Tribhuvan University (TU), Kirtipur, Kathmandu, Nepal.
  • Google Scholar
Ram Lal Shrestha
  • Ram Lal Shrestha
  • Nepal Agriculture Research Council (NARC), Lalitpur, Nepal.
  • Google Scholar
Sujan Chaudhary
  • Sujan Chaudhary
  • Department of Botany, Amrit Science Campus, Kathmandu, Nepal.
  • Google Scholar
Sangita Shrestha
  • Sangita Shrestha
  • Molecular Biotechnology Unit, Faculty of Science, Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal.
  • Google Scholar


  •  Received: 01 November 2021
  •  Accepted: 05 January 2022
  •  Published: 30 April 2022

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