African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Propagation of cochineal scale insect free cactus (Opuntia ficus-indica) by in vitro regeneration culture technique in Tigray, Ethiopia

Tsehaye Kidus Ebuy
  • Tsehaye Kidus Ebuy
  • Department of Quality Assurance, Research and Development, Tigray Biotechnology Center Pvt. Ltd. Co, Mekelle, Ethiopia.
  • Google Scholar
Kiros–Meles Ayimut
  • Kiros–Meles Ayimut
  • College of Dry land Agriculture and Natural Recourse, Mekelle University, Mekelle, Ethiopia.
  • Google Scholar


  •  Received: 13 October 2022
  •  Accepted: 13 February 2023
  •  Published: 31 July 2023

References

Akram G, Tahereh H, Mojtaba KN (2013). Micro propagation of tuna (Opuntia ficus-indica) and the effect of medium composition on proliferation and rooting. International Journal of Biosciences 3(11):129-139.
Crossref

 

Ault JR, Blackmon WJ (1987). In vitro propagation of Ferocactus acanthodes (Cactaceae). HortScience 22(1):126-127.
Crossref

 

Bhau BS (1999). Regeneration of Coryphantha elephantidens (Lem.) Lem. (Cactaceae) from root explants. Scientia Horticulturae 81:337-344.
Crossref

 

Bhau BS, Wakhlu AK (2015). A highly efficient in vitro propagation protocol for elephant tusk cactus: Coryphantha elephantidens (Lem.) Lem. Journal of Genetic Engineering and Biotechnology 13(2):215-219.
Crossref

 

Clayton PW, Hubstenberger JF, Phillips GC (1990). Micropropagation of members of cactaceae subtribe cactinae. Horticultural Science 115(2):337-343.
Crossref

 

Escobar HA, Vitor M, Villalobos A, Villegas A (1986). Opuntia micropropagation by axillary proliferation. Plant Cell, Tissue and Organ Culture 7.269-277.
Crossref

 

Estrada-Luna AA, Mart?nez-Hernandez JJ, Torres-Torres ME, Chable-Moreno F (2008). In vitro micro propagation of the ornamental prickly pear cactus Opuntia lanigera salm-dyck and effects of sprayed GA3 after transplantation to ex vitro conditions. Scientia Horticulturae 117(4):378-385.
Crossref

 

Fay MF, Gratton J (1992). Tissue culture of cacti and other succulents: a literature review and a report on micro propagation at Kew. Bradleya 10:33-48.
Crossref

 

Felker P, Paterson A, Jenderek MM (2006). Forage Potential of Opuntia Clone Maintained by the USDA, National Plant Germplasm System (NPGS) Collection. Crop Science 46(5):2161-2168.
Crossref

 

Flores-Valde'z CA (1995). ''Nopalitos'' production, processing and marketing. In: Barbera G, Inglese P, Pimienta-Barrios E, ed. Agro ecology, Cultivation and Uses of Cactus Pear, FAO, Rome pp. 92-99.

 

García-Saucedo PA, Valdez-Morales M, Elena Valverde M, Cruz-Hernandez A, Paredes-Lopez O (2005). Plant regeneration of three Opuntia genotypes used as human food. Plant Cell, Tissue and Organ Culture 80:215-219.
Crossref

 

Guadalupe M, Humberto S, Ralph B (1999). In vitro culture as a potential method for the conservation of endangered plants possessing crassulacean acid metabolism. Scientia Horticulturae 81(1):71-87.
Crossref

 

Habtu L (2005). Cactus in southern Tigray: Current status, potential uses, utilization, and threat. An M.Sc. a thesis presented to Addis Ababa University School of graduate studies. Addis Ababa, Ethiopia.

 

Hubstenberger JF, Clayton PW, Phillips GC (1992). Micropropagation of cacti (Cactaceae). Biotechnology in agriculture and forestry.

 

Lema-Ruminska J, Kulus, Haseltonia D (2014). Micropropagation of cacti-a review. Haseltonia19:46-63.
Crossref

 

Juárez MC, Passera CB (2002). In vitro propagation of Opuntia ellisiana Griff and acclimatization to field conditions. Biocell 26(3):319-324.
Crossref

 

Kibra K (1992). Agro-ecological constraints and the role of cactus pear on household food availability. M.Sc. Thesis, Agricultural University of Norway.

 

Khalafalla MM, Abdellatef E, Mohammed Ahmed MM, Osman MG (2007). Micropropagation of cactus (Opuntia ficus-indica) as a strategic tool to combat desertification in arid and semi-arid regions. International Journal of Sustainable Crop Production 2(4):3.

 

Le Houérou HN (2000). Utilization of fodder trees and shrubs in the arid and semiarid zones of west Asia and North Africa. Arid Soil Research and Rehabilitation 14(2):101-135.
Crossref

 

Llamoca-Za' rate RM, Aguiar LF, Landsmann J, Campos FAP (1999). Establishment of callus and cell suspension cultures of Opuntia ficus-indica. Plant Cell, Tissue and Organ Culture 58:155-157.
Crossref

 

Malda G, Suzan H, Backhaus R (1999). In vitro culture as a potential method for the conservation of endangered plants possessing crassulacean acid metabolism. Scientia Horticulturae 81(1):71-87.
Crossref

 

Martinez-Vazquez O, Rubluo A (1989). In vitro mass propagation of the near extinct Mammillaria san-angelensis Snchez-Mejorada. Journal of Horticultural Science 64(1):99-105.
Crossref

 

Mauseth JD (1977). Cactus tissue culture: a potential method of propagation. Cactus and Succulent Journal 49:80-81.

 

Mauseth JD (1979). A new method for the propagation of cacti: the sterile culture of axillary buds. Cactus and Succulent Journal 51:186-187.

 

Meaza T (2009). Contributions of prickly pear cactus towards achieving household food security in the Tigray region of Ethiopia: the case of Tabia Kihen, Kilete Awelaelo Woreda. An M.sc. a thesis presented to Addis Abeba University School of graduate studies. Addis Ababa, Ethiopia.

 

Moran VC, Cobby BS (1979). On the life-history and fecundity of cochineal insect, dactylopius austrinus Di Lotto (Homoptera: dactylopiidae) a biological control agent for the cactus Opuntia aurantiaca. Bulletin of Entomological Research 69(4):629-636.
Crossref

 

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15(3):473-497.
Crossref

 

Nobel PS (1995). Environmental Biology. In: Agro-ecology, Cultivation, and Uses of Cactus Pear. FAO Plant Production and Protection Paper, FAO, Rome pp. 36-48.

 

Palomino G, Doležel J, Cid R, Brunner I, Méndez I, Rubluo A (1999). Nuclear genome stability of Mammillaria san-angelensis (Cactaceae) regenerants induced by auxins in long-term in vitro culture. Plant Science 141:191-200.
Crossref

 

Pérez-Molphe-Balch E, Santos-Díaz MS, Ramírez-Malagón R, Ochoa-Alejo N (2015). Tissue culture of ornamental cacti. Scientia Agricola 72: (6):540-561.
Crossref

 

Rojas-Are' chiga M, Vasquez Yanes C (2000). Cactus seed germination: a review. Journal of Arid Environments 44:85-104.
Crossref

 

Scheinvar L (1995). Taxonomy of utilized Opuntias. In: Agro ecology, cultivation, and uses of cactus pear. Barbera GP. Inglese, and E. Pimienta-Barrios (eds). FAO Plant Production and protection paper 132. Rome, Italy pp. 20-27.

 

Tesfay B, Gebreselassie M, Abadi T (2011). Description of cactus pear (Opuntia ficus-indica (l.) mill.) cultivars from Tigray, northern Ethiopia some cactus (Opuntia ficus-indica) varieties identified. Research Report 1:8.