African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

A protocol for large scale genomic DNA isolation for cacao genetics analysis

Rogério Mercês Ferreira Santos
  • Rogério Mercês Ferreira Santos
  • Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna-BA, Brazil.
  • Google Scholar
Uilson Vanderlei Lopes
  • Uilson Vanderlei Lopes
  • Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna-BA, Brazil.
  • Google Scholar
Didier Clement
  • Didier Clement
  • Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna-BA, Brazil; CIRAD, UMR AGAP, Avenue Agropolis TA96/03, 34398 Montpellier cedex 5, France.
  • Google Scholar
Jose Luis Pires
  • Jose Luis Pires
  • Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna-BA, Brazil.
  • Google Scholar
Eline Matos Lima
  • Eline Matos Lima
  • Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna-BA, Brazil; Universidade Estadual de Santa Cruz (UESC), 45662-900 Ilhéus-BA, Brazil.
  • Google Scholar
Tamiles Batista Messias
  • Tamiles Batista Messias
  • Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna-BA, Brazil; Universidade Estadual de Santa Cruz (UESC), 45662-900 Ilhéus-BA, Brazil.
  • Google Scholar
Karina Peres Gramacho
  • Karina Peres Gramacho
  • Cocoa Research Center, CEPLAC/CEPEC, 45600-970 Itabuna-BA, Brazil.
  • Google Scholar


  •  Accepted: 05 January 2014
  •  Published: 12 February 2014

References

Almeida CMVC, Machado PFR, Barriga JB, Silva FCO (1995). Coleta de cacau (Theobroma cacao L.) da Amazônia brasileira: uma abordagem histórica e analítica. CEPLAC/SUPOC, Porto Velho, Brasil.
 
Chase MW, Hills HG (1991). Silica gel: an ideal material for field preservation of leaf samples for DNA studies. Taxon 40:215–220.
Crossref
 
Faleiro FG, Santos IS, Bahia RCS, Santos RF, Yamada MM, Anhert D (2002). Otimização da extração e amplificação de DNA de Theobroma cacao L. visando a obtenção de marcadores RAPD. Agrotrópica 14: 31-34.
 
Fang G, Hammer S, Grumet R (1992). A quick and inexpensive method for removing polysaccharides from plant genomic DNA. BioTechniques 13:52–55.
Pubmed
 
Figueira A, Janick J, Goldsbrough P (1992). Genome size and DNA polymorphism in Theobroma cacao. J. Am. Soc. Hort. Sci. 117: 673-677.
 
Guiltinan M (2007). Cacao. In: Pua VE, Davey M (eds) Biotechnology in Agriculture and Forestry - Transgenic Crops. Springer-Verlag, Berlin, German. pp. 152-163.
 
Lanaud C (1986). Utilisation des marqueurs enzymatiques pour étude genetique do cacaoyer. Cafe Cacao Thea 30: 259-270.
 
Lanaud C, Risterucci AM, N'Goran JAK, Clement D, Flament M, Laurent V, Falque M (1995). A genetic linkage map of Theobroma cacao L. Theor. AppI. Genet. 91: 987-993.
 
Laurent V, Risterucci AM, Lanaud C (1993). Variability for molecular ribosomal genes within Theobroma cacao. Heredity 71: 96-103.
Crossref
 
Maximova SN, Tan CL, Guiltinan MJ (2007). Cocoa In: Kole C, Hall TC (eds) A Compendium of Transgenic Crop Plants. Blackwell Publishing, Malden, MA, USA.
 
Micheli F, Guiltinan M, Gramacho KP, Figueira AV, Cascardo JCM, Maximova S, Lanaud C (2010). Functional genomics of cacao. Adv. Bot. Res. 155:119–177.
Crossref
 
Murray MG, Thompson WF (1980). Rapid isolation of high molecular weight DNA. Nucleic Acids Res. 8: 4321-4325.
Crossref
 
Pires JL, Monteiro WR, Pinto LRM, Luz EDMN (1999). Resistance to witches' broom- Evaluation of genotypes from different origins. In: Proceedings of the 12th International Cocoa Research Conference, Salvador, Brazil, 1996. Cocoa Producers Alliance, London, UK.
 
Risterucci AM, Grivet L, N'Goran JAK, Pieretti I, Flament MH, Lanaud C (2000). A high density linkage map of Theobroma cacao L. Theor. Appl. Gen. 101:948 -955.
Crossref
 
Sambrook J, Fritsch EF, Maniatis T (1989). Molecular Cloning: A laboratory Manual. 2nd Ed. Cold Spring Laboratory, Cold Spring Harbor, New York, USA.
 
Tai TH, Tanksley SD (1988). A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue. Plant Mol. Biol. Rep. 8:297-303.
Crossref
 
Tel-Zur NS, Abbo S, Myslabodksi D, Mizrahi Y (1999). Modified CTAB procedure for DNA isolation from epiphytic cacti of the genera Hylocereusand Selenicereus (Cactaceae). Plant Mol. Biol. Rep. 17: 249-254.
Crossref
 
Yamada MM, Faleiro FG, Lopes UV, Bahia RC, Pires JL, Gomes LMC, Melo GRP (2001). Genetic variability in cultivated cacao populations in Bahia, Brazil, detected by isozymes and RAPD markers. Crop Breed. Appl. Biot. 4:377-384.
Crossref