Detection of eight common β-globin gene mutation in thalassemia major patients using real time polymerase chain reaction (PCR)-high resolution melting and EvaGreen™ dye
Seyed Jalal Marashi1*, Sima Ataollahi Eshkoor1, Mir saed Mirinargesi1,2, Mohammad Reza Sarookhani3, Asmah Bt. Rahmat4 and Patimah Bt. Ismail1
1Molecular Biology Laboratory, Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
2Department of Genetics, Faculty of Medicine, Tonekabon Islamic Azad University, Tonekabon, Iran.
3School of Public Health and Paraclinics, Qazvin University of Medical Sciences, Qazvin, Iran.
4Department of Nutrition, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Malaysia.
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