African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12218

Table of Content: 11 June, 2014; 13(24)

June 2014

Application of random amplified polymorphic DNA (RAPD) markers to identify Taxus chinensis var. mairei cultivars associated with parthenogenesis

The random amplified polymorphic DNA (RAPD) technique has been widely applied to identify different varieties of plants for molecular breeding. However, application of RAPD markers to identify parthenogenesis in plants has not been reported. In this investigation, we used pedigree and RAPD markers to differentiate different Taxus chinensis var. mairei cultivars that were associated with parthenogenesis. Among 180 and...

Author(s): Yongjun Fei, Wei Tang, Jinhua Shen, Tianjing Zou, Rui Qi, Bo Xiao, Cunyu Zhou, Zhixiong Liu and Anna Y. Tang

June 2014

Genome polymorphism markers and stress genes expression for identifying turf species

Reactive oxygen species (ROS) are produced in both stressed and unstressed cells. Superoxide dismutase (SOD) and phenylalanine ammonia lyase (PAL) play an important role in the defense against ROS. Eight different turf grass species were used in order to detect their ability to withstand environmental stress through investigating SOD and PAL gene expression and also the genetic relationship among them using random...

Author(s): Hany M. El-Naggar

June 2014

Combining ability and heterosis for phenologic and agronomic traits in maize (Zea mays L.) under drought conditions in the Northern Guinea Savanna of Borno State, Nigeria

Five International Institute of Tropical Agriculture (IITA) drought tolerant and open pollinated varieties (OPVs) (lines) and four local varieties with various level of susceptibility to drought (testers) were used. These maize varieties were crossed using a line x tester mating design during the 2007 cropping season to determine the general combining ability (GCA) and specific combining ability (SCA); also the level of...

Author(s): D. Aminu Y. M. Garba and A. S. Muhammad

June 2014

Effect of Dodonaea viscosa Jacq. residues on growth and yield of mungbean (Vigna mungo L. Hepper)

This study was to evaluate the effect of Dodonaea viscosa Jacq. residues on mungbean (Vigna mungo L.Hepper) local cultivar. An experiment [using randomized complete block design (RCBD) design] with three replications was conducted in 2010. The trial comprised of four treatments such as mulching, incorporation into soil and extract, along with control with mungbean crop without adding residues of D. viscose. Data showed...

Author(s): Kamil M. M. Al-Jobori and Sumeia Abbas Ali

June 2014

In vitro propagation of the new disease resistant Coffea arabica variety, Batia

Batian is a true breeding commercial coffee variety that was released in Kenya in 2010. It is resistant to coffee berry disease and coffee leaf rust which are the main coffee diseases in Kenya. Coupled with early ripening, good beverage quality and high yields, demand for planting material has surpassed supply. Conventional propagation methods do not provide enough planting materials, hence the need to develop...

Author(s): Lubabali, A. H., Alakonya, A. E., Gichuru, E. K., Kahia, J. W. and Mayoli, R. N.

June 2014

Studies on seed yield potential of some selected kenaf (Hibiscus cannabinus L.) genotypes

Kenaf seed yield depends on morpho-physiological traits between varieties, and the interaction between genotype and the environment. Studies were conducted in Ibadan, Ilora and Kisi stations of the Institute of Agricultural Research and Training (IAR&T), Obafemi Awolowo University, Moor Plantation, Ibadan, Oyo State, Nigeria during 2009 and 2010 cropping seasons to determine the influence of location and genotypes...

Author(s): J. O. Olasoji, O. A. Aluko, G. O. Agbaje, O. N. Adeniyan, K. O. Kareem and S. O. Olanipekun

June 2014

Physiological characteristics and pathogenicity of Xanthomonas campestris pv. musacearum strains collected from enset and banana in Southwest Ethiopia

and banana plants since the first record in Keffa province in Southwest Ethiopia as early as the 1960s. The disease remains a dominant constraint to enset production although its impact on banana has declined over the past four decades. The disease is ravaging banana plantations and spreading at alarming rates since its recent outbreak in other east and central African countries, including Uganda, Democratic Republic of...

Author(s): Befekadu Haile, Girma Adugna and Fikre Handoro

June 2014

Influence of temperature, pH, and ionic strength on the rheological properties of oviductus ranae hydrogels

The main rheological features of oviductus ranae (OR) hydrogels were investigated as a function of temperature, pH and ionic strength. With respect to the steady shear measurements, a reduction in viscosity was observed as the temperature increased. The flow behavior can be well described by the Herschel-Bulkley model in the down curve at a shear rate of 300 to 0 s−1, where standard error is lower than 20, and it...

Author(s): Qing Liang, Jinsong Zhang, Changjiang Xu, Jianpeng Dou and Shouqin Zhang

June 2014

Extraction, partial purification and characterization of pectinases isolated from Aspergillus species cultured on mango (Mangifera indica) peels

Pectinase was produced from a culture of Aspergillus niger, Aspergillus fumigatus and Aspergillus flavus. Pectinase synthesis was achieved using mango (Mangifera indica) pectin extract as an inducer during pectinolytic fungi isolation while submerged fermentation process was carried out using ground mango peels as the sole carbon source. Substrate fermentation was evaluated within seven days by monitoring the pectinase...

Author(s): Daniel Ikenna UDENWOBELE, Chukwunonso Anthony NSUDE, Arinze Linus EZUGWU, Sabinus Oscar Onyebuchi EZE, Chukwudi ANYAWU, Peter Nzemndu UZOEGWU and Ferdinand Chiemeka CHILAKA

June 2014

Expression and characterization of a novel spore wall protein from Nosema bombycis

Microsporidia are obligate intracellular, eukaryotic, spore-forming parasites. The environmentally resistant spores, which harbor a rigid cell wall, are critical for their survival outside their host cells and host-to-host transmission. The spore wall comprises two major layers: the exospore and the endospore. In Nosema bombycis, five spore wall proteins have been identified which contain two endosporal proteins (SWP25...

Author(s): Haihong Qiu, Mingqian Li , Xinyi He, Xiangkang He and Xingmeng Lu

June 2014

Ascorbic acid and mineral elements composition of powdered antimalarial (Maloff-HB) and haematinic (Haematol-B) herbal formulations from Ogbomoso, Nigeria

Different types of powdered antimalarial and blood-enriching (haematinic) recipes are used in traditional health system of Southwestern Nigeria. Two of these from Ogbomoso (that is Maloff-HB and Haematol-B, respectively) were recently named following a quantitative definition of their botanical constituents. However, information on the physico-chemical and phytochemical properties as well as the residual constituents of...

Author(s): Adepoju Tunde Joseph OGUNKUNLE, Olugbenga Solomon BELLO and Adijat Funke OGUNDOLA

June 2014

Antidiabetic effect of aqueous extract of Basella alba leaves and metformin in alloxan-induced diabetic albino rats

The present study was carried out to evaluate the anti-diabetic effect of aqueous extract of Basella alba leaves in alloxan-induced diabetic albino rats. Thirty (30) male albino rats weighing 100 to 150 g were used for this work. The rats were randomly distributed into five groups containing six (6) rats per group. Group I rats served as the healthy control group and were neither induced with diabetes nor given any...

Author(s): Bamidele, O., Arokoyo, D. S., Akinnuga, A. M. and Oluwarole, A. O.