African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Application of random amplified polymorphic DNA (RAPD) markers to identify Taxus chinensis var. mairei cultivars associated with parthenogenesis

Yongjun Fei
  • Yongjun Fei
  • College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, People’s Republic of China.
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Wei Tang
  • Wei Tang
  • College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, People’s Republic of China.
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Jinhua Shen
  • Jinhua Shen
  • College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, People’s Republic of China.
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Tianjing Zou
  • Tianjing Zou
  • College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, People’s Republic of China.
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Rui Qi
  • Rui Qi
  • College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, People’s Republic of China.
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Bo Xiao
  • Bo Xiao
  • College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, People’s Republic of China.
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Cunyu Zhou
  • Cunyu Zhou
  • College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, People’s Republic of China.
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Zhixiong Liu
  • Zhixiong Liu
  • College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, People’s Republic of China.
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Anna Y. Tang
  • Anna Y. Tang
  • 101 Stadium Drive, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.
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  •  Received: 20 January 2014
  •  Accepted: 26 May 2014
  •  Published: 11 June 2014

Abstract

The random amplified polymorphic DNA (RAPD) technique has been widely applied to identify different varieties of plants for molecular breeding. However, application of RAPD markers to identify parthenogenesis in plants has not been reported. In this investigation, we used pedigree and RAPD markers to differentiate different Taxus chinensis var. mairei cultivars that were associated with parthenogenesis. Among 180 and eighty RAPD primers used, 108 primers generated RAPD bands from genomic DNA of T. chinensis var. mairei cultivars (“Jingzhou” and “Baokang”). Six hundred and thirty-three RAPD loci bands were produced and used to identify eight cultivars with unique banding patterns. Pedigree and RAPD data demonstrated that T. chinensis var. mairei cultivars with parthenogenesis were separated from others in both “Jingzhou” and “Baokang” cultivars. These results provide evidence for identification of parthenogenesis and confirm that the RAPD technique is especially suitable for identification of T. chinensis var. mairei cultivars for agricultural purposes.

 

Key words: Cultivar identification, DNA extraction, parthenogenesis, pedigree, random amplified polymorphic DNA (RAPD), Taxus chinensis var. mairei.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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