African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12182

Table of Content: 11 October 2010; 9(41)

October 2010

Investigation of the ultrastructural and histopathological changes in coronary arteries of German shepherd dogs following alloxan induced diabetes mellitus

Diabetes mellitus is one of the most common disease of the endocrine system in the body that is diagnosed by metabolic malfunction in the metabolism of carbohydrates, fat and proteins. This disease involves most tissues of the body and the consequent deficiencies reduce their efficiency, cause infections and diseases in the body. Alloxan is a chemical which is used in inducing experimental diabetes in animals. In this...

Author(s): Mohammadreza Valilou and Alireza Lotfi

October 2010

Cloning and functional analysis in transgenic tobacco of a tapetum-specific promoter from Arabidopsis

The 5’-flanking region of 1174 bp upstream of the translation start point (TSP) of a reported Arabidopsis anther-specific gene, Anther7 gene (ATA7), which putatively encodes a protein related to lipid transfer protein, was cloned and functionally analyzed in transgenic tobacco after been fused with β-glucuronidase (GUS) gene reporter. Histochemical GUS staining of the transgenic plants...

Author(s): Xuan-Li Nie, Jian-Wei Zhu, An-Qi Geng and Xing-Guo Xiao

October 2010

Proteome analysis of human colorectal cancer tissue using 2-D DIGE and tandem mass spectrometry for identification of disease-related proteins

Laser capture microdissection and two-dimensional difference gel electrophoresis were used to establish the proteomic profiles for tumor and matched adjacent tissues from 12 patients. Differential protein spots were identified by mass spectrometric analysis. The cDNA of the differential protein was transfected into colorectal cancer cells, and the biological behavior of these cells was observed. The proteomic profile in...

Author(s): Xue Bai, Shi-Yong Li, Bo Yu, Ping An, Hui-Yun Cai and Jun-Feng Du

October 2010

Analysis of bovine growth hormone gene polymorphism of local and Holstein cattle breeds in Kerman province of Iran using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP)

Bovine growth hormone (bGH) gene is a part of the multiple gene family that contains prolactin and placental lactogens. Also, variations in introns have potential usefulness as genetic markers and could help in the genetic improvement of populations. Genomic DNA was isolated from blood samples of two local herds (53 animals) and two Holstein herds (50 animals). Genomic DNA samples were genotyped for the GHI-AluI...

Author(s): Mohammadabadi M.R,  Torabi A,  Tahmourespoor M,  Baghizadeh A,  Esmailizadeh Koshkoieh A and  Mohammadi A

October 2010

Studies on plant regeneration and transformation efficiency of Agrobacterium mediated transformation using neomycin phosphotransferase II (nptII) and glucuronidase (GUS) as a reporter gene

Plant transformation mediated by Agrobacterium tumefaciens, a soil plant pathogenic bacterium, is the most used method for the introduction of foreign genesinto plant cells and the subsequent regeneration of transgenic plants. We have standardized the tissue culture media for the regeneration and transformation with the vector LBA 4404 (pCAMBIA 2301), so that in future, this system may be exploited...

Author(s): Shefali Dobhal, Dinesh Pandey, Anil Kumar and Sanjeev Agrawal

October 2010

Physicochemical and biochemical characteristics evaluation of seven improve cassava (Manihot esculenta crantz) varieties of Côte d'Ivoire

A lot of people around the world convert cassava roots into different products according to local customs and preferences. In Côte d’Ivoire, due to its high consumption, cassava is a subject of concern to researchers. For that purpose, seven improved varieties of cassava (with better productivity and resistance to many diseases), were set up by CNRA. Nevertheless, their valorization and popularization need...

Author(s): Séverin Kra Kouassi, Rose-Monde Mégnanou, Eric Essoh Akpa, Cathérine Djedji, N’zué B. and Sébastien Niamké L.

October 2010

Effects of the weed density on grass yield of Alfalfa (Medicago sativa L.) in different row spacing applications

This study, in which the effects of different row spacing applications on weed density and on grass yield of Medicago sativa L. were investigated, was carried out in Van-Turkey from 2006 - 2008. Randomized blocks design was adopted with three replications. Row spacing applications of 20, 30, 40, 50, 60 and 70 cm were tested. The alfalfa plant height, yield of green herbage yield, dry matter yield, crude...

Author(s): Seyda Zorer Celebi, llhan Kaya, A. Korhan Sahar and Reyyan Yergin

October 2010

Quality and yield response of soybean (Glycine max L. Merrill) to drought stress in sub–humid environment

The aim of the study was to determine the response of soybean [Glycine max (L.) Merr.] to drought at various stages of development in a sub-humid environment of Turkey. Drought-stress treatments was applied to plants in 2005 and 2006 by withholding irrigation at six critical stages: completely vegetative (fifth trifoliate) (T2), flowering (T3), podding (T4), seed fill (T5), full bloom + podding (T6), and podding +...

Author(s): Çiğdem Demirtaş, Senih Yazgan, Burak Nazmi Candogan, Mehmet Sincik, Hakan Büyükcangaz and Abdurrahim Tanju Göksoy

October 2010

Bioaugmentation efficiency of diesel degradation by Bacillus pumilus JLB and Acinetobacter calcoaceticus LT1 in contaminated soils

The abilities of diesel-degrading Bacillus pumilus JLB and Acinetobacter calcoaceticus LT1 were tested in contaminated soils. The effect of nutrient supplementation on bioaugmented samples was also examined. The results show that bio-augmentation and biostimulation accelerated significantly (p < 0.05) the diesel degradation in the contaminated loam soil and sea sand. Supplementing...

Author(s): C. Singh and J. Lin

October 2010

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by white rot-fungus Pseudotrametes gibbosa isolated from the boreal forest in Northeast China

This study compared laccase production and the degradation of polycyclic aromatic hydrocarbons (PAHs) by aboriginal white rot-fungus Pseudotrametes gibbosa(found in the northeast forest area of China) and Pleurotus ostreatus (which has been studied both domestically in China and overseas). The results showed that the laccase activity of P. gibbosa was 2841.3 U/l, which was 6 times more...

Author(s): Dawen Gao, Hong Liang, Lina Du and Jun Chen

October 2010

Quality assessment and antimicrobial activity of various honey types of Pakistan

Forty samples of different honey types (Acacia, Ziziphus, Brassica and Citrus) were collected from different areas of Pakistan and analyzed for moisture, pH, total acidity, ash, electrical conductivity, hydroxymethylfurfural (HMF), sucrose, total sugars, invert sugar, protein, proline contents as well as macro and micro elements. The variation in composition of honey samples was observed due to different types of flora....

Author(s): Muhammad Gulfraz, Farida Iftikhar, Shazia Raja, Saira Asif, Sajid Mehmood, Zahid Anwar and Ghazala Kaukob

October 2010

Modeling metabolic response to changes of enzyme amount in yeast glycolysis

Based on the work of Hynne et al. (2001), in an in silico model of glycolysis,Saccharomyces cerevisiae is established by introducing an enzyme amount multiple factor (ɑ) into the kinetic equations. The model is aimed to predict the metabolic response to the change of enzyme amount. With the help of ɑ, the amounts of twelve enzymes were altered by different multiples from their initial values....

Author(s): Xuelian Yang, Lin Xu and Ming Yan

October 2010

Textural properties of infra red dried apple slices as affected by high power ultrasound pre-treatment

Drying is a process frequently used in food industry, often based on the use of conventional methods using heat exchange by conduction or convection. This kind of method may lead to quality loss in structure, texture and sensory characteristics of final products. Consequently, the need for research of new drying methods arises. One of such methods is power ultrasound aided drying. The aim of this work was to investigate...

Author(s): Brncic M, Karlovic S, Rimac Brncic S, Penava A, Bosiljkov T, Ježek D and Tripalo B

October 2010

Soil carbon dioxide (CO2) efflux of two shrubs in response to plant density in the northern Loess Plateau of China

Although plant density should affect soil carbon dioxide (CO2) efflux and carbon cycling in semi-arid regions, the effects of plant density on soil CO2 efflux are not well known. This study was performed to investigate the responses of soil CO2efflux of two dominant shrubs (Caragana korshinkii and Salix psammophila) to plant density in the northern Loess Plateau of China. Two plant density...

Author(s): Wei Fu,, Mingbin Huang, Ming,an Shao, and Robert Horton

October 2010

Comparative analysis of the chemical composition of three spices – Allium sativum L. Zingiber officinale Rosc. and Capsicum frutescens L. commonly consumed in Nigeria

The beneficial health effects of spices against common chronic systemic diseases have been well documented. Comparative study of the proximate, mineral and phytochemical components of three spices namely garlic (Allium sativum L.), ginger (Zingiber officinale Rosc.) and pepper (Capsicum frutescens L.) were investigated. Analysis of the proximate composition revealed that the spices had considerable...

Author(s): Gloria A. Otunola, Oyelola B. Oloyede Adenike T. Oladiji and Anthony J. Afolayan

October 2010

The effects of salt stress on the growth, biochemical parameter and mineral element content of some maize (Zea mays L.) cultivars

Six cultivars of maize (Zea mays L.) (Ada-523, Bora, C-955, PR 3394, Progen-1550 and Trebbia) were subjected to 0 and 100 mM NaCl and their response to salt stress were determined by growths related to relative shoot growth weight (RSGR), shoot and root dry weight and stress tolerance index by biochemical parameters associated with total chlorophyll and proline contents and by mineral element contents such as...

Author(s): Emine Budakli Carpici, Necmettin Celik, Gamze Bayram and Bulent Baris Asik

October 2010

Preliminary phytochemical, antimicrobial and acute toxicity studies of the stem, bark and the leaves of a cultivated Syzygium cumini Linn. (Family: Myrtaceae) in Nigeria

The phytochemical, antimicrobial and acute toxicity studies of the leaf and stem bark extracts of Syzygium cumini Linn. grown in Abuja, Nigeria was carried out. The phytochemical screening revealed the presence of carbohydrates, saponins, tannins, terpenes, volatile oil, sterols, resins balsam, phlobatanins and flavonoids in the leaves and stem bark of the species studied. Alkaloids and anthraquinones were...

Author(s): Ugbabe, G.E, Ezeunala, M.N, Edmond, I.N, Apev, J and Salawu, O.A

October 2010

Effect of delayed sterilization on the production of intravenous fluids (parenterals)

The effect of delayed sterilization on the production of intravenous fluids using 5%(w/v) dextrose solution was studied. The 5% (w/v) dextrose was prepared with distilled water. The solution was inoculated with a laboratory isolate of Escherichia coli and thereafter divided into two: Solutions A and B. Sample A was sterilized within 1 h of innoculation, while B was incubated at 37°C for...

Author(s): Salawu, M. O, Oloyede, O. B, Oladiji, A. T,Yakubu, M. T and Atata R.F

October 2010

Effects of aqueous extract of Basella alba leaves on haematological and biochemical parameters in albino rats

The effects of the aqueous leaf extract of Basella alba on haematological and biochemical parameters were studied in Wistar strain albino rats. Twenty four (24) Wistar strain albino rats were randomly distributed into four groups of six (6) rats each. Group  = 1 \* ROMAN I  rats served as control and received 10 ml/kg of normal saline, while group  = 2 \* ROMAN II,  = 3 \* ROMAN III...

Author(s): Bamidele, O., Akinnuga, A. M., Olorunfemi, J. O., Odetola, O. A., Oparaji, C. K. and Ezeigbo, N.

October 2010

Ultrastructure of antennal sensillae of the samsum ant, Pachycondyla sennaarensis (Hymenoptera: Formicidae)

Black ant (Samsum), Pachycodyla sennarrensis, stings and injects venom and inflicts allergy (a rare clinical problem) due to its local and systemic reaction, which is considered as a health hazard amongst Saudi society. Thus, black ant is a source of serious concern for the government and experts as well. Ultramorphological variations, distribution, differential sensillae counts (DSC) and total sensillae counts...

Author(s): Mohammed I. Siddiqui, Ashraf M. A. Mashaly, Ashraf M. Ahmed, Fahd A. Al-Mekhlafi and Mohamed S. Al-Khalifa

October 2010

Survey of Trojan fir seed orchard in Edremit-Gurgendag Region of Turkey

Abies nordmanniana ssp. Equitrojani, with a limited distribution in Northwestern Anatolia (Kazdaglari Mountain ranges), is one of the four Abies taxa naturally grown in Turkey. Regeneration problems are caused when establishing seed orchards for this important conifer. Seed and cone pests are the main problems encountered in orchards. This study was conducted between the years of 2006 and 2008 in Edremit-Gurgendag Seed...

Author(s): Hamit Ayberk and Huseyin Cebeci

October 2010

Bioaccumulation of some heavy metals and histopathological alterations in liver of Euryglossa orientalis and Psettodes erumei along North Coast of the Persian Gulf

In order to make evaluation of some heavy metals bioaccumulation and explore their histopathological effects on hepatocytes of oriental sole (Euryglossa orientalis) and deep flounder (Psettodes erumei), fishes were caught from two areas of north coast of the Persian Gulf, Bandar Abbass and Bandar Lengeh. Concentrations of nickel (Ni) and vanadium (V) in liver of both species in two sampling regions were in the following...

Author(s): Zahra Khoshnood, Amin Mokhlesi and Reza Khoshnood

October 2010

Potentials of short term and long term cryopreserved sperm of the African giant catfish (Clarias gariepinus Burchell, 1822) for aquaculture

To service the growing demand for male African giant catfish (Clarias gariepinus) broodstock for aquaculture in Nigeria, and to conserve valuable genetic resources, we improved both short-term (in deep freezer at -35°C) and long-term cryopreservation (in liquid nitrogen at -296°C) of catfish sperm. Catfish sperm cryopreserved at -35°C using three different types of cryodiluents composed of 10% methanol,...

Author(s): O. G. Omitogun, O. F. Olaniyan, O.O. Oyeleye, C. Ojiokpota, S.E. Aladeleand W. T. Odofin

October 2010

Virulence of Bacillus cereus as natural facultative pathogen of Anopheles subpictus Grassi (Diptera: Culicidae) larvae in submerged rice-fields and shallow ponds

Out of 4407 Anopheles subpictus larvae collected from submerged rice-fields and shallow ponds, 1412 were found to be unhealthy and 2.8% of unhealthy larvae were naturally infected by Bacillus cereus. B. cereus formed circular, white and flat colonies. Bacteria were gram positive, ellipsoidal/oval spore forming aerobic rods. Although the isolate was positive for catalase, urease,...

Author(s): Soumendranath Chatterjee, Tuhin Subhra Ghosh and Surajit Das

October 2010

Assessment of the efficacies, potencies and bacteriological qualities of some of the antibiotics sold in Calabar, Nigeria

In this study, an assessment of the efficacies, potencies and qualities of 11 brands of 5 different antibiotics including 3 brands of ampiclox and 2 brands each ofciprofolxacin, gentamicin, rifampicin and tetracylcine sold in Calabar, South-South region of Nigeria was carried out using the agar diffusion technique (sensitivity testing). The efficacies, potencies and qualities of these...

Author(s): A. O. Nkang, I. O. Okonko, J. A. Lennox, O. D. Eyarefe, M. J. Abubakar, M. O. Ojezele E. T. Babalola, T. A. Ogunnusi,B. I. Onajobi andT. A. Amusan

October 2010

The effect of glutamine supplement on small intestinal morphology and xylose absorptive ability of weaned piglets

The purpose of this study is to demonstrate the effects of glutamine (Gln) supplement on small intestinal morphology, xylose absorptive and growth performance of weaned piglets. Forty eight piglets weaned at 28 ± 2 days of age were randomly allotted to three treatment groups. A basal corn-soybean diet was formulated to contain 20.3% protein and 3450 kcal DE/kg diet. Glutamine was supplemented to the basal diet at...

Author(s): C. B. Hsu, , H. J. Huang, C. H. Wang H. T. Yen and B. Yu

October 2010

Variation in the chemical composition of the essential oils of different organs of domesticated Lippia multiflora Moldenke

The essential oils from the different organs of wild and domesticated Lippia multiflora Moldenke, analysed by GPC-FID and GPC-MS, were rich in monoterpenes. These made up 95.0, 94.0, 82.5 and 61.0% of oils from leaves, flowers, stems and roots, respectively, with a predominance of aromatic...

Author(s): Gouollaly Tsiba, Nkounkou Loumpangou Célestine, Mahmout Yaya, Ouamba Jean-Maurille, Abena Ange Antoine, Chalchat Jean-Claude and Figueredo Gilles

October 2010

Genetic diversity of indigenous chicken ecotypes in Jordan

DNA polymorphism of 4 indigenous chicken ecotypes was assessed in Jordan using random amplified polymorphic DNA (RAPD) markers. 10 RAPD markers showed high genetic diversity values in the 4 ecotypes located in the northern, eastern, western and southern provinces of Jordan. The effective number of alleles per locus ranged from 1.47 to 1.7 (mean 1.65). The expected heterozygosity varied from 0.28 to 0.41 (mean 0.39) and...

Author(s): Raed Al-Atiyat

October 2010

Caulimoviral sequences in Dahlia variabilis in Egypt

The presence of Dahlia mosaic virus D10 (DMV-D10) was confirmed for the first time in dahlia (Dahlia variabilis) in Egypt. DMV-D10 was recently described as a caulimovirus that exists as an endogenous pararetroviral sequence (EPRS). DMV-D10 was confirmed by amplification of the ORF1 (encoding for the movement protein) using species specific primers (D10F1/R1). The expected size (900 bp) was...

Author(s): A. M. Abdel-Salam, M. M. Al Khazindar, S. G. Eid and H.R. Pappu