African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12212

Table of Content: 13 December 2010; 9(50)

December 2010

Roles of DNA methyltransferases in Arabidopsis development

  DNA methylation plays a vital role during development in gene expression and chromatin organization. DNA methyl transferases catalyze the transfer of a methyl group to bases within the DNA helix. Plants differ from animals in having methylation at the sites of CHG and CHH. In plant, there are at least four classes of cytosine methyltransferase: MET1, CMT3, DRM and DNMT2. They show distinct...

Author(s): Junjun Huang, Huahua Wang, Xiaojun Xie, Dan Zhang, Yan Liu and Guangqin Guo

December 2010

Mapping of QTLs for leaf area and the association with winter hardiness in fall-sown lentil

  ariations in plant architecture are often associated with the ability of plants to survive cold stress during winter. In studies of winter hardiness in lentil, it appeared that small leaf area was associated with improved winter survival. Based on this observation, the inheritance of leaf area and the relationship with winter hardiness using an F6 - derived recombinant inbred line (RIL) population from...

Author(s):   Abdullah Kahraman, Ufuk Demirel, Mustafa Ozden and F. J. Muehlbauer  

December 2010

Research on prokaryocyte expression and biological activity of the core region of Bloom’s Syndrome protein

  To establish an effective approach for inducing expression of the RecQ core of Bloom’s Syndrome protein (BLM642-1290) and assaying its biological activity in vitro, BLM642-1290 recombinant protein was expressed with IPTG at room temperature inEscherichia coli, and then the expressed product was assayed using SDS-PAGE and western blotting. After purification via affinity chromatography, DNA...

Author(s):   Xiang Chen, Heng Luo, Li-Xia Duan, Yong Zhang, and Hou-Qiang Xu  

December 2010

Diversity analysis of the developed qingke (hulless barley) cultivars representing different growing regions of the Qinghai-Tibet Plateau in China using sequence-related amplified polymorphism (SRAP) markers

  Genetic diversity among 68 accessions of the developed qingke (hulless barley) cultivars from Sichuan, Gansu, Tibet, Qinghai and Yunnan provinces of the Qinghai-Tibet Plateau in China was evaluated by using a newly developed sequence-related amplified polymorphism (SRAP) marker system. The results showed that, 20 primer combinations produced a total of 350 clear bands with an average of 17.5 bands per primer...

Author(s): Ping Yang,#, Xianjun Liu ,#, Xinchun Liu , Wuyun Yang and Zongyun Feng,

December 2010

Genetic divergence among African and American cotton (Gossypium hirsutum L. race latifolium H.) cultivars and inbred lines through random amplification of polymorphic DNA (RAPD) markers.

Cotton (Gossypium spp.) is an important cash crop and the second largest source of textile fiber and edible oil throughout the world. This study was conducted to investigate the genetic divergence through random amplified polymorphism (RAPD) molecular markers among the introduced African and American cultivars and inbred lines of cotton (Gossypium hirsutum L. raça latifolium H.)...

Author(s):   Manuel Pedro Maleia, Pedro Soares Vidigal Filho, Maria Celeste Gonçalves-Vidigal, Adriana Gonela, Giselly Figueiredo Lacanallo, Leonel Domingos Moiana, António Chamuene, Lorenna Lopes de Sousa and Luana Mieko Darben.  

December 2010

An improved calcium chloride method preparation and transformation of competent cells

  Transformation is one of the fundamental and essential molecular cloning techniques. In this paper, we have reported a modified method for preparation and transformation of competent cells. This modified method, improved from a classical protocol, has made some modifications on the concentration of calcium chloride and competent bacteria solution, rotation speed in centrifugation and centrifugation time. It...

Author(s): Xiaowei Li, Xin Sui, Yan Zhang, Yepeng Sun, Yan Zhao, Ying Zhai and Qingyu Wang

December 2010

The comparison of immunomodulatory effects of peripheral mononuclear cells against proliferation in U937 in junior elderly habitual morning swimming in Taiwan cohort

  The present investigation was carried out to evaluate the effect of greater immunomodulatory effects of human peripheral blood mononuclear cells against proliferation in human leukemia cells.  To achieve this, cells U937 in junior elderly (with cool environmental physical activities) subjects with habitual morning swimming and sedentary lifestyle were recruited in relatively cool season in Taiwan; the...

Author(s): A. C. Huang, C. M. Jiang, C. C. Lee, T. S. Huang, M. C. Wu and Y. Y. Chen  

  • Article Number: ED40A0922782

December 2010

The preparation of 6x His-tagged granulocyte colony stimulating factor using an improved in vitro expression

  An improved in vitro expression system called the rapid translation system (RTS) was used in this study for the in vitro biosynthesis of 6x His tagged granulocyte colony stimulating factor (6x His-tagged granulocyte colony-stimulating factor (GCSF)). This was done to overcome the problems associated with traditional cell based biotechnology. The study involved the preparation of template...

Author(s):   Helmy, O. M., Hussein, M. M. M., Murad, F. E. and Shoeb, H. A.  

December 2010

The earlier identification of the seedless characteristic of the wampee [Clausena lansium (Lour.) Skeels] hybrid by a random amplified polymorphic DNA (RAPD) marker

  Wampee as an important fruit is widely grown in South China and conventional breeding methods for the production of new seedless wampee cultivars in recent years were operated. Hybridization was conducted using the seed as female parents and the seedless as male parents. However, the seeds could be gotten from cross combination. An efficient operation was that, non-woven fabrics bag could increase burliness...

Author(s): Zhao Zhichang,, Hu Guibing, Ou Yangruo, Liu Yunchun, Yi Yang and Chen Yeyuan

December 2010

Genome-wide detection of Ty1-copia and Ty3-gypsy group retrotransposons in Japanese apricot (Prunus mume Sieb. et Zucc.)

  The conserved domains of reverse transcriptase (RT) genes of approximately 260 bp for Ty1-copia and 430 bp for Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from Japanese apricot (Prunus mume Sieb. et Zucc.) using degenerate oligonucleotide primers. Sequence analysis showed that 32.3% of Ty1-copia and 27.5%...

Author(s):   Fei Wang#, Zhaoguo Tong#, Jun Sun, Yuying Shen, Jun Zhou, Zhihong Gao and Zhen Zhang  

December 2010

Detection of Arisaema yunnanense as adulterant of traditional medicine Pinellia ternata using allele-specific diagnostic polymerase chain reaction (PCR)

  The dried tubers of Pinellia ternata (Araceae) have been widely used in traditional Chinese, Japanese and Korean medicine. Recently, Pinellia tuber has been found to be adulterated with Arisaema yunnanense in south West China due to its natural resource deficiency. To distinguish between Pinellia tuber and A. yunnanense, the chloroplast atpB-rbcL...

Author(s):   Tao Liu and Xiaorong Guo  

December 2010

Alleviation of salinity effects by exogenous applications of salicylic acid in pearl millet (Pennisetum glaucum (L.) R. Br.) seedlings

  Experiments for the study of alleviation of salt stress effects by exogenous applications of salicylic acid (SA) in pearl millet (Pennisetum glaucum (L.) R. Br.) seedlings were conducted in soil filled earthen pots having size 12 inches with 7 kg soil. Three treatments comprising, control (T0), 50-mol m-3 NaCl (T1) and T2 are having NaCl (50 mol m-3) + SA (50 mg l-1). Pots were arranged in...

Author(s): Khalid Hussain, Khalid Nawaz, Abdul Majeed, Farah Khan, Feng Lin, Abdul Ghani, Ghulam Raza, Shahid Afghan, Syed Zia-ul-Hussnain, Kazim Ali and Aamir Shahazad

December 2010

Effects of lead (PbCl2) stress on germination of lentil (Lens culinaris Medic.) lines

  The presence of heavy metals in solid and liquid wastes is an important issue that is related to the pollution of the environment. They are one of the most important environmental pollutants and reaching dangerous amount requires more research. This study was conducted to investigate the effects of lead (PbCl2) stress on germination of different lentil lines. Seeds of four different lentil (Lens...

Author(s):   Alihan Cokkizgin and Hatice Cokkizgin  

December 2010

In vitro micrografting of Sterculia setigera Del.

  An in vitro procedure micrografting of adult scions of Sterculia setigera was developed to overcome low rooting in adult shoot. Axenic micro shoots of 0.5 cm length taken from adult trees as scions were grafted on seedlings rootstocks cultured on MS medium. 100% success was obtained with micrografts using adultapex as scions. Upon three cycles of in vitro micrografting, rejuvenation...

Author(s):   Dame Niang, Yaye Kène Gassama, Aliou Ndiaye, Maurice Sagna, Samba Arona Ndiaye Samba and Mamoudou Abdoul Toure  

December 2010

Assessment of diversity and plant growth promoting attributes of rhizobia isolated from Cajanus cajan L.

  Eight (8) strains of rhizobia from Pigeon pea (Cajanus cajan) were isolated and identified based on their physiological and biochemical characteristics using standard methods. Five (5) of the isolates (KCC1 to KCC5) were subjected to partial 16S rDNA gene sequencing for phylogenetic analysis. Genetic diversity was investigated using restriction patterns produced by amplified rDNA restriction...

Author(s):   R. C. Dubey, D. K. Maheshwari, Harish Kumar and Kamlesh Choure  

December 2010

Base catalyzed transesterification of sunflower oil biodiesel

  In this study, sunflower oil was investigated for biodiesel production. Sunflower is one of the leading oil seed crop, cultivated for the production of oil in the world. It has also been considered as an important crop for biodiesel production. Seeds for biodiesel production were procured from local farmers of Attock and Rawalpidni divisions. Electric oil expeller was used for the extraction of crude oil....

Author(s):   Mushatq Ahmad, Shoaib Ahmed, Fayyaz-Ul-Hassan, Muhammad Arshad, Mir Ajab Khan, Muhammad Zafar and Shazia Sultana  

December 2010

Intercrop performance of different varieties of soybean (Glycine Max. (L) Merril) in a cassava (Manihot esculenta Crantz) based cropping system within the derived savannah zone

  Field experiments to investigate intercrop performance of different varieties of soybean (Glycine Max. (L.) Merril) in a cassava (Manihot esculenta Crantz) based cropping system within the derived savannah zone were conducted at Nsukka Utisol in Southeast Nigeria ecological. Two varieties of cassava and six varieties of soybean of three different maturity groups were grown as sole and intercrop...

Author(s): S. I. Umeh and B. N. Mbah

  • Article Number: 7FF1B0023548

December 2010

Effect of plant density and cultivars on growth, yield and yield components of faba bean (Vicia faba L.)

  One field experiment was conducted at the Experimental Farm, Faculty of Agriculture at Zabol University during the 2006/2007 season to study the effect of plant density (D) and cultivars (V) on some growth characters of faba bean. The experimental design applied was randomized complete block (RCBD) in factorial arrangement with three replications. Plant density at three levels (12.5, 16.7 and 20 plant m-2)...

Author(s):   Mehdi Dahmardeh, Mahmood Ramroodi and Jafar Valizadeh  

December 2010

Phytoremediation technologies for Ni++ by water hyacinth

  Phytoremediation of metal contaminated soil and water by Eichhornia crassipes(water hyacinth) is promising. The study was conducted to compare the phytoremoval of Ni++ from soil and wastewater. For the measurement of phytoremoval from soil, E. crassipes was used in a pot experiment. Results showed the removal of (Ni) 24.23 µg/g dry weight of plant and large level calculations show...

Author(s):   Tariq Mahmood, Syed Tajammul Hussain and Salman Akbar Malik  

December 2010

Investigating the effects of two lignocellulose degradation by-products (furfural and acetic acid) on ethanol fermentations by six ethanologenic yeast strains

  The effects of two lignocellulose degradation by-products (furfural and acetic acid) on ethanol fermentations by six ethanologenic yeast strains were investigated. It was found that 1.5 g/l of furfural and 4.8 g/l of acetic acid, especially the latter, inhibited cell growth and increased ethanol yield, significantly. On the other hand, different yeast strains have different cell growth rate but very similar...

Author(s): Wu Zheng-Yun, Deng Yu, Tan Li, Liu Yue-hong, Zang Yi-jie and Zhang Wen-Xue

  • Article Number: E5CC81623644

December 2010

MnFe2O4/bentonite nano composite as a novel magnetic material for adsorption of acid red 138

  Magnetic MnFe2O4/bentonite nanocomposite was synthesized by chemical co-precipitation method. The product was characterized by X-ray diffraction (XRD) and Scanning electron microscope (SEM). XRD results indicated the presence of free quartz in bentonite. The magnetic ferrite MnFe2O4 has spinel structure. It is also found that the presence of bentonite in the magnetic composite has not made any changes in...

Author(s): Saeedeh Hashemian

December 2010

Pre-staining thin layer chromatography method for amino acid detection

  Thin layer chromatography (TLC) is an analytical method that is used commonly. It usually includes five steps: spotting, separating, drying, spraying/immersing and color development. In this paper, TLC was modified with pre-staining method, which only consisted of 3 steps: spotting, separating and color development. The modified thin layer chromatography can be used for the analysis of amino acids. When...

Author(s): Ting Qiu, Haixing Li and Yusheng Cao  

December 2010

Lauric acid and 2, 6-ditertbutyl phenol, two major allelochemicals from Rehmannia glutinosa inhibiting the germination of succeeding crop, Sesamum indicum

Rehmannia glutinosa Libosch, a well known herb for Chinese traditional medicine, and sesame are two main crops in Northwestern Henan, China. It has been known however that, a rotation of these two crops, for example, cultivation of sesame preceeded with R. glutinosa Libosch, leads to decreased yield and poor quality of sesame. In order to identify the compound or allelochemicals which inhibit the growth...

Author(s): Ming Dao Wang, Hongge Chen, Xinyu Liu, Yuqian Gao, Kun Wu and Xincheng Jia

December 2010

Immunomodulatory effects of orally administered aqueous extract from Eupolyphaga sinensis Walker

  This paper reported the immunnodulation tests of Eupolyphaga sinensis Walker in immunity. Mice were fed with E. sinensis water-decoction at a dose of 1.89, 3.78 and 7.56 g/kg/d for 4 weeks, then their immune function was studied. The results of the effects of E. sinensis water-decoction on spleen index, thymus index and carbon expurgation experiments of mice, indicated...

Author(s):   Qingfeng Tang, Yin Dai and Xuelan Liu  

December 2010

Proximate composition of fresh water bighead carp, Aristichthys nobilis, in relation to body size and condition factor from Islamabad, Pakistan

Seventy-six Bighead carp, Aristichthys nobilis, of different body sizes were selected for analysis of body composition parameters’ variables in relation to body size and condition factor. Each fish was measured, weighed, dried and powdered for the analysis of water, ash, fat and protein contents. It was observed that for Aristichthys nobilis, significant inverse correlation existed between water content...

Author(s): Muhammad Naeem and Abdus Salam  

December 2010

Direct shoot organogenesis and plant regeneration from cotyledonary node of kenaf (Hibiscus cannabinus L.)

  Using the whole cotyledonary node as explants, a rapid and efficient regeneration protocol was established for kenaf (Hibiscus cannabinus L.) on Murashige and Skoog (MS) basal medium. The effects of the plant growth regulators: N6-benzyladenine (BA), indole-3-aceticacid (IAA) and the non-ironic surfactant pluronic F-68 (F-68) on shoot regeneration were evaluated by the orthogonal design L16 (45). The...

Author(s):   Peng Chen, Ru Li, Ruiyang Zhou and Yang Zhao  

December 2010

Machine induced changes of caprine teats diagnosed by ultrasonography

  Analyses were performed on 10 nanny goats of the Polish White Improved breed aged 2 to 5 years. Ultrasound images of the longitudinal cross-section of teats were taken in 9 replications, 4 times daily before and immediately after morning milking, and then 4 and 10 h after milking, using a Hitachi EUB405+ apparatus with a 10 MHz linear probe. The ultrasound probe was placed in a plastic cup filled with...

Author(s): Piotr Ślósarz, Jacek Wójtowski, Sylwia Bielińska, Anna Frąckowiak, Agnieszka Ludwiczak, Józef Krzyżewski, Emilia Bagnicka and Nina Strzałkowska

December 2010

Study on relationship between microsatellite polymorphism and producing ability on litter size trait of Hu sheep in China

Four microsatellite loci (OarAE101, BM1329, BM143 and OarHH55) linked to FecB gene on chromosome 6 and one microsatellite locus (OarHH55) on chromosome 4 were selected to study their correlation with litter size of Hu sheep breed. The results showed that the average polymorphism information content (PIC), heterozygosity (He) and effective allele number (Ne) were 0.7214, 0.7558 and 4.4094, respectively, and all 5...

Author(s):   W. Sun, H. Chang, Hassan Hussein Musa and Mingxing Chu