December 2010
Roles of DNA methyltransferases in Arabidopsis development
DNA methylation plays a vital role during development in gene expression and chromatin organization. DNA methyl transferases catalyze the transfer of a methyl group to bases within the DNA helix. Plants differ from animals in having methylation at the sites of CHG and CHH. In plant, there are at least four classes of cytosine methyltransferase: MET1, CMT3, DRM and DNMT2. They show distinct...
December 2010
Mapping of QTLs for leaf area and the association with winter hardiness in fall-sown lentil
ariations in plant architecture are often associated with the ability of plants to survive cold stress during winter. In studies of winter hardiness in lentil, it appeared that small leaf area was associated with improved winter survival. Based on this observation, the inheritance of leaf area and the relationship with winter hardiness using an F6 - derived recombinant inbred line (RIL) population from...
December 2010
Research on prokaryocyte expression and biological activity of the core region of Bloom’s Syndrome protein
To establish an effective approach for inducing expression of the RecQ core of Bloom’s Syndrome protein (BLM642-1290) and assaying its biological activity in vitro, BLM642-1290 recombinant protein was expressed with IPTG at room temperature inEscherichia coli, and then the expressed product was assayed using SDS-PAGE and western blotting. After purification via affinity chromatography, DNA...
December 2010
Diversity analysis of the developed qingke (hulless barley) cultivars representing different growing regions of the Qinghai-Tibet Plateau in China using sequence-related amplified polymorphism (SRAP) markers
Genetic diversity among 68 accessions of the developed qingke (hulless barley) cultivars from Sichuan, Gansu, Tibet, Qinghai and Yunnan provinces of the Qinghai-Tibet Plateau in China was evaluated by using a newly developed sequence-related amplified polymorphism (SRAP) marker system. The results showed that, 20 primer combinations produced a total of 350 clear bands with an average of 17.5 bands per primer...
December 2010
Genetic divergence among African and American cotton (Gossypium hirsutum L. race latifolium H.) cultivars and inbred lines through random amplification of polymorphic DNA (RAPD) markers.
Cotton (Gossypium spp.) is an important cash crop and the second largest source of textile fiber and edible oil throughout the world. This study was conducted to investigate the genetic divergence through random amplified polymorphism (RAPD) molecular markers among the introduced African and American cultivars and inbred lines of cotton (Gossypium hirsutum L. raça latifolium H.)...
December 2010
An improved calcium chloride method preparation and transformation of competent cells
Transformation is one of the fundamental and essential molecular cloning techniques. In this paper, we have reported a modified method for preparation and transformation of competent cells. This modified method, improved from a classical protocol, has made some modifications on the concentration of calcium chloride and competent bacteria solution, rotation speed in centrifugation and centrifugation time. It...
December 2010
The comparison of immunomodulatory effects of peripheral mononuclear cells against proliferation in U937 in junior elderly habitual morning swimming in Taiwan cohort
The present investigation was carried out to evaluate the effect of greater immunomodulatory effects of human peripheral blood mononuclear cells against proliferation in human leukemia cells. To achieve this, cells U937 in junior elderly (with cool environmental physical activities) subjects with habitual morning swimming and sedentary lifestyle were recruited in relatively cool season in Taiwan; the...
December 2010
The preparation of 6x His-tagged granulocyte colony stimulating factor using an improved in vitro expression
An improved in vitro expression system called the rapid translation system (RTS) was used in this study for the in vitro biosynthesis of 6x His tagged granulocyte colony stimulating factor (6x His-tagged granulocyte colony-stimulating factor (GCSF)). This was done to overcome the problems associated with traditional cell based biotechnology. The study involved the preparation of template...
December 2010
The earlier identification of the seedless characteristic of the wampee [Clausena lansium (Lour.) Skeels] hybrid by a random amplified polymorphic DNA (RAPD) marker
Wampee as an important fruit is widely grown in South China and conventional breeding methods for the production of new seedless wampee cultivars in recent years were operated. Hybridization was conducted using the seed as female parents and the seedless as male parents. However, the seeds could be gotten from cross combination. An efficient operation was that, non-woven fabrics bag could increase burliness...
December 2010
Genome-wide detection of Ty1-copia and Ty3-gypsy group retrotransposons in Japanese apricot (Prunus mume Sieb. et Zucc.)
The conserved domains of reverse transcriptase (RT) genes of approximately 260 bp for Ty1-copia and 430 bp for Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from Japanese apricot (Prunus mume Sieb. et Zucc.) using degenerate oligonucleotide primers. Sequence analysis showed that 32.3% of Ty1-copia and 27.5%...
December 2010
Detection of Arisaema yunnanense as adulterant of traditional medicine Pinellia ternata using allele-specific diagnostic polymerase chain reaction (PCR)
The dried tubers of Pinellia ternata (Araceae) have been widely used in traditional Chinese, Japanese and Korean medicine. Recently, Pinellia tuber has been found to be adulterated with Arisaema yunnanense in south West China due to its natural resource deficiency. To distinguish between Pinellia tuber and A. yunnanense, the chloroplast atpB-rbcL...
December 2010
Alleviation of salinity effects by exogenous applications of salicylic acid in pearl millet (Pennisetum glaucum (L.) R. Br.) seedlings
Experiments for the study of alleviation of salt stress effects by exogenous applications of salicylic acid (SA) in pearl millet (Pennisetum glaucum (L.) R. Br.) seedlings were conducted in soil filled earthen pots having size 12 inches with 7 kg soil. Three treatments comprising, control (T0), 50-mol m-3 NaCl (T1) and T2 are having NaCl (50 mol m-3) + SA (50 mg l-1). Pots were arranged in...
December 2010
Effects of lead (PbCl2) stress on germination of lentil (Lens culinaris Medic.) lines
The presence of heavy metals in solid and liquid wastes is an important issue that is related to the pollution of the environment. They are one of the most important environmental pollutants and reaching dangerous amount requires more research. This study was conducted to investigate the effects of lead (PbCl2) stress on germination of different lentil lines. Seeds of four different lentil (Lens...
December 2010
In vitro micrografting of Sterculia setigera Del.
An in vitro procedure micrografting of adult scions of Sterculia setigera was developed to overcome low rooting in adult shoot. Axenic micro shoots of 0.5 cm length taken from adult trees as scions were grafted on seedlings rootstocks cultured on MS medium. 100% success was obtained with micrografts using adultapex as scions. Upon three cycles of in vitro micrografting, rejuvenation...
December 2010
Assessment of diversity and plant growth promoting attributes of rhizobia isolated from Cajanus cajan L.
Eight (8) strains of rhizobia from Pigeon pea (Cajanus cajan) were isolated and identified based on their physiological and biochemical characteristics using standard methods. Five (5) of the isolates (KCC1 to KCC5) were subjected to partial 16S rDNA gene sequencing for phylogenetic analysis. Genetic diversity was investigated using restriction patterns produced by amplified rDNA restriction...
December 2010
Base catalyzed transesterification of sunflower oil biodiesel
In this study, sunflower oil was investigated for biodiesel production. Sunflower is one of the leading oil seed crop, cultivated for the production of oil in the world. It has also been considered as an important crop for biodiesel production. Seeds for biodiesel production were procured from local farmers of Attock and Rawalpidni divisions. Electric oil expeller was used for the extraction of crude oil....
December 2010
Intercrop performance of different varieties of soybean (Glycine Max. (L) Merril) in a cassava (Manihot esculenta Crantz) based cropping system within the derived savannah zone
Field experiments to investigate intercrop performance of different varieties of soybean (Glycine Max. (L.) Merril) in a cassava (Manihot esculenta Crantz) based cropping system within the derived savannah zone were conducted at Nsukka Utisol in Southeast Nigeria ecological. Two varieties of cassava and six varieties of soybean of three different maturity groups were grown as sole and intercrop...
December 2010
Effect of plant density and cultivars on growth, yield and yield components of faba bean (Vicia faba L.)
One field experiment was conducted at the Experimental Farm, Faculty of Agriculture at Zabol University during the 2006/2007 season to study the effect of plant density (D) and cultivars (V) on some growth characters of faba bean. The experimental design applied was randomized complete block (RCBD) in factorial arrangement with three replications. Plant density at three levels (12.5, 16.7 and 20 plant m-2)...
December 2010
Phytoremediation technologies for Ni++ by water hyacinth
Phytoremediation of metal contaminated soil and water by Eichhornia crassipes(water hyacinth) is promising. The study was conducted to compare the phytoremoval of Ni++ from soil and wastewater. For the measurement of phytoremoval from soil, E. crassipes was used in a pot experiment. Results showed the removal of (Ni) 24.23 µg/g dry weight of plant and large level calculations show...
December 2010
Investigating the effects of two lignocellulose degradation by-products (furfural and acetic acid) on ethanol fermentations by six ethanologenic yeast strains
The effects of two lignocellulose degradation by-products (furfural and acetic acid) on ethanol fermentations by six ethanologenic yeast strains were investigated. It was found that 1.5 g/l of furfural and 4.8 g/l of acetic acid, especially the latter, inhibited cell growth and increased ethanol yield, significantly. On the other hand, different yeast strains have different cell growth rate but very similar...
December 2010
MnFe2O4/bentonite nano composite as a novel magnetic material for adsorption of acid red 138
Magnetic MnFe2O4/bentonite nanocomposite was synthesized by chemical co-precipitation method. The product was characterized by X-ray diffraction (XRD) and Scanning electron microscope (SEM). XRD results indicated the presence of free quartz in bentonite. The magnetic ferrite MnFe2O4 has spinel structure. It is also found that the presence of bentonite in the magnetic composite has not made any changes in...
December 2010
Pre-staining thin layer chromatography method for amino acid detection
Thin layer chromatography (TLC) is an analytical method that is used commonly. It usually includes five steps: spotting, separating, drying, spraying/immersing and color development. In this paper, TLC was modified with pre-staining method, which only consisted of 3 steps: spotting, separating and color development. The modified thin layer chromatography can be used for the analysis of amino acids. When...
December 2010
Lauric acid and 2, 6-ditertbutyl phenol, two major allelochemicals from Rehmannia glutinosa inhibiting the germination of succeeding crop, Sesamum indicum
Rehmannia glutinosa Libosch, a well known herb for Chinese traditional medicine, and sesame are two main crops in Northwestern Henan, China. It has been known however that, a rotation of these two crops, for example, cultivation of sesame preceeded with R. glutinosa Libosch, leads to decreased yield and poor quality of sesame. In order to identify the compound or allelochemicals which inhibit the growth...
December 2010
Immunomodulatory effects of orally administered aqueous extract from Eupolyphaga sinensis Walker
This paper reported the immunnodulation tests of Eupolyphaga sinensis Walker in immunity. Mice were fed with E. sinensis water-decoction at a dose of 1.89, 3.78 and 7.56 g/kg/d for 4 weeks, then their immune function was studied. The results of the effects of E. sinensis water-decoction on spleen index, thymus index and carbon expurgation experiments of mice, indicated...
December 2010
Proximate composition of fresh water bighead carp, Aristichthys nobilis, in relation to body size and condition factor from Islamabad, Pakistan
Seventy-six Bighead carp, Aristichthys nobilis, of different body sizes were selected for analysis of body composition parameters’ variables in relation to body size and condition factor. Each fish was measured, weighed, dried and powdered for the analysis of water, ash, fat and protein contents. It was observed that for Aristichthys nobilis, significant inverse correlation existed between water content...
December 2010
Direct shoot organogenesis and plant regeneration from cotyledonary node of kenaf (Hibiscus cannabinus L.)
Using the whole cotyledonary node as explants, a rapid and efficient regeneration protocol was established for kenaf (Hibiscus cannabinus L.) on Murashige and Skoog (MS) basal medium. The effects of the plant growth regulators: N6-benzyladenine (BA), indole-3-aceticacid (IAA) and the non-ironic surfactant pluronic F-68 (F-68) on shoot regeneration were evaluated by the orthogonal design L16 (45). The...
December 2010
Machine induced changes of caprine teats diagnosed by ultrasonography
Analyses were performed on 10 nanny goats of the Polish White Improved breed aged 2 to 5 years. Ultrasound images of the longitudinal cross-section of teats were taken in 9 replications, 4 times daily before and immediately after morning milking, and then 4 and 10 h after milking, using a Hitachi EUB405+ apparatus with a 10 MHz linear probe. The ultrasound probe was placed in a plastic cup filled with...
December 2010
Study on relationship between microsatellite polymorphism and producing ability on litter size trait of Hu sheep in China
Four microsatellite loci (OarAE101, BM1329, BM143 and OarHH55) linked to FecB gene on chromosome 6 and one microsatellite locus (OarHH55) on chromosome 4 were selected to study their correlation with litter size of Hu sheep breed. The results showed that the average polymorphism information content (PIC), heterozygosity (He) and effective allele number (Ne) were 0.7214, 0.7558 and 4.4094, respectively, and all 5...
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