African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

In vitro micrografting of Sterculia setigera Del.

  Dame Niang1*, Yaye Kène Gassama1, Aliou Ndiaye1, Maurice Sagna1, Samba Arona Ndiaye Samba2 and Mamoudou Abdoul Toure2  
  1Laboratoire Campus de Biotechnologies Végétales, Département  de Biologie Végétale, Faculté des Sciences & Techniques, Université Cheikh Anta Diop de Dakar, BP 5005,  Dakar-Fann, Sénégal. 2Laboratoire National de Recherches sur les Productions Végétales-Institut Sénégalais de Recherches Agricoles (LNRPV -ISRA), Route des Hydrocarbures, Bel Air, B.P. 3120, Dakar, Sénégal.
Email: [email protected]

  •  Accepted: 18 August 2010
  •  Published: 13 December 2010

Abstract

 

An in vitro procedure micrografting of adult scions of Sterculia setigera was developed to overcome low rooting in adult shoot. Axenic micro shoots of 0.5 cm length taken from adult trees as scions were grafted on seedlings rootstocks cultured on MS medium. 100% success was obtained with micrografts using adultapex as scions. Upon three cycles of in vitro micrografting, rejuvenation capacities of S. setigera was recovered as shown by vigour, length and rooting of shoots grown from grafts cultured on MS medium compared to seedlings. Successful micrografts were transferred to plastic pots containing soil under mist house conditions before they were finally exposed to an external environment. 80% of the plantlets survived in the nursery.

 

Key words: Sterculia setigera, micrografting, rejuvenation, rooting.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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