June 2017

Value addition of orange fruit wastes in the enzymatic production of xylooligosaccharides

Xylooligosaccharides (XOS) are sugar oligomers made up of xylose units with the chain length of 2 to 10 and are considered non-digestible food ingredients. It is mainly produced from xylan hydrolysis. XOS have a characteristic of prebiotic by promoting the growth of probiotic organisms. They have various physiological effects such as reducing cholesterol, maintaining the gastrointestinal health and improving the...

Author(s): Praveen Kumar Gupta, Pushpa Agrawal and Prabha Hegde

June 2017

Immunocapture and simple-direct-tube-reverse transcriptase polymerase chain reaction (RT-PCR) for detection of Rice yellow mottle virus

This study aimed at optimizing the immunocapture (IC) and simple-direct-tube (SDT) -reverse transcriptase polymerase chain reaction (RT-PCR) techniques for detection of Rice yellow mottle virus (RYMV) in order to avoid the extraction of high quality RNA required for molecular methods and avoid costs involved. Rice yellow mottle virus strains and phylotypes were obtained from infected rice leaf samples collected from...

Author(s): Judith Hubert, Herman John Faraji Lyimo, Ashura Luzi-Kihupi and Ernest Rashid Mbega

June 2017

Phytochemical screening and amino acids analysis of mushrooms from Burkina Faso

Mushrooms play an important social, economic and ecological role in the world. In this study, the proximate compositions, minerals and amino acids contents of Russula alveolata, Russula cf. compressa, Russula flavobrunnea var. aurantioflava and Russula ochrocephala from Burkina Faso were investigated. The chemical analysis showed the presence of volatile oil, sterols and triterpenes, carotenoids, saponosides, reducing...

Author(s): Elise Sanon, Jean Claude W. Ouédraogo, Sylvain Ilboudo, Marie K. Laure Guissou, Pierre I. Guissou and Philippe Sankara

June 2017

Establishment, multiplication, rooting and acclimatization of Cabralea canjerana (Vell.) Martius

Seedling production of canjerana (Cabralea canjerana (Vell.) Martius) has been limited by difficulty in germination, caused by recalcitrant behavior of their seeds. The objective of this study was to develop an efficient protocol for conservation and production of canjerana micropropagated plantlets. Seeds of canjerana were disinfected with 0, 2.5, 5.0, 7.5 and 10% of NaOCl solution, at immersion times of 10, 20 and 30...

Author(s): Eliseo Salvatierra Gimenes, Nathalia Pimentel, James Hancock and Dilson Antônio Bisognin

June 2017

Optimised cetyltrimethylammonium bromide (CTAB) DNA extraction method of plant leaf with high polysaccharide and polyphenolic compounds for downstream reliable molecular analyses

Yam (Dioscorea species) and banana (Musa species) leaf sample contain high levels of polysaccharide and polyphenolic compounds. Extraction of good quality DNA from such leaves is usually problematic. Therefore, there is a need to extract good quality DNA in order to perform downstream DNA analysis, especially for the detection of viruses due to their low titration in some infected leaves. The cetyltrimethylammonium...

Author(s): Turaki A. A.,, Ahmad B., Magaji U. F., Abdulrazak U. K., Yusuf B. A. and Hamza A. B.,