African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12340

Table of Content: 18 December 2006; 5(24)

December 2006

Marker assisted introgression of opaque2 gene into herbicide resistant elite maize inbred lines

Marker assisted selection in combination with conventional breeding can greatly accelerate the introgression of modified opaque2 genotype into herbicide resistant maize. By combining these two approaches, time and costs are greatly minimized.  The application of opaque2 allele specific SSR markers was done on materials already undergoing selection in a breeding program for converting herbicide resistant maize...

Author(s): Jedidah W. Danson, Mercy Mbogori, Michael Kimani, Martin Lagat, Alex Kuria and Alpha Diallo

December 2006

Development of a simple and powerful method, cDNA AFLP-SSPAG, for cloning of differentially expressed genes

A novel plant differentially expressed gene cloning technique called cDNA-AFLP-SSPAG was described. Using this method, Differential mRNA expression was found between high and low heterosis groups of maize. Differential cDNAs were easily obtained from silver stained cDNA-AFLP separated on polyacylamide gels. The cDNA was then reamplified, cloned and fragments were sequenced. Sequenced clones were used as probes in...

Author(s): Minsheng Wu

December 2006

Antioxidant activity of the methanolic extracts of some species of Phlomis and Stachys on sunflower oil

Antioxidant effects of the methanolic extract of Phlomis bruguieri, P. herba-venti, P. olivieri, Stachys byzantina, S. inflata, S. lavandulifolia and S. laxa were tested in sunflower oil stored at 70ºC, by mea-suring peroxide values after regular intervals and compared with rosemary-, green tea- and BHA-containing samples. The methanolic extracts of P....

Author(s): Katayoun Morteza-Semnani, Majid Saeedi and Somayeh Shahani

December 2006

Purification and characterization of a protease from Thermophilic bacillus strain HS08

The purification and characterization of a thermophilic neutral protease fromThermophilic bacillus strain HS08, originally isolated from a soil sample collected from the Tulufan Crater of China, is presented in this paper. The purification steps included ammonium sulfate precipitation, with columns of DEAE-Sepharose anion exchange chromatography and Sephacryl S-100HR on AKTA purifier 100 protein liquid...

Author(s): HUANG Guangrong, YING Tiejing, HUO Po and JIANG Jiaxing

December 2006

Comparative pesticidal activity of dichloromethane extracts of Piper nigrum against Sitophilus zeamais and Callosobruchus maculatus

Piperine a clinically established pesticide was investigated in the seeds, fruit and leaves of Piper nigrum. The dichloromethane extracts obtained from the parts of the plant were analysed through Thin Layer Chromatography (TLC) using silica gel-GF 254 impregnated aluminium plate and various solvent system consisting of chloroform : methanol (8:2), (6:4), ethyl acetate : methanol (9:1), and acetone : hexane (6:4)...

Author(s): O. A. Awoyinka I. O. Oyewole, B. M. W. Amos and O. F. Onasoga

December 2006

Occurrence and functioning of phosphate solubilizing microorganisms from oil palm tree (Elaeis guineensis) rhizosphere in Cameroon

Phosphorus replenishment, particularly in smallholder agriculture, remains a challenge as it is mainly fertilizer dependent. While the use of soluble mineral phosphate fertilizers is the obvious best means to combat phosphate deficiency in Cameroon, their use is limited by their high cost and availability at farmer’s level. This study was carried out aimed at maintaining the fertility of Cameroon soils by...

Author(s): Henri Fankem, , Dieudonné Nwaga, Annette Deubel, Lamine Dieng, Wolfgang Merbach and Francois Xavier Etoa

December 2006

Biochemical evaluation of Cassipourea congoensis (Tunti) and Nuclea latifolia (Luzzi) fruits

The fruits of Cassipourea congoensis (Tunti) and Nuclea latifolia (Luzzi) were assessed chemically for the presence of mineral elements, vitamins A, B, C, E, and some antinutritional factors. Results obtained showed that C. congoensis had higher quantities of Cu, Co, Fe, Ca, Mg and Mn ( 0.25 ± 0.02, 0.52 ± 0.01, 6.70 ± 0.13,...

Author(s): I. I. Nkafamiya, A. J. Manji, U. U. Modibbo and H. A. Umaru

December 2006

Comparative in-vitro studies on antiplasmodial quality of some Nigerian and foreign brands of chloroquine oral formulations marketed in kano

The world Health Organization’s committee for the review of medicines encourages a continuous post-marketing surveillance for quality of essential drugs among which chloroquine is one. In compliance with that, samples of oral chloroquine brands belonging to twenty manufacturers both foreign and Nigerian, ten in each case that were on sale in Kano market between January and August, 2004 were investigated for...

Author(s): M.D Mukhtar, M Bashir and A.H. Arzai

December 2006

A study on gumbo seed grown in Congo Brazzaville for its food and industrial applications

Proximate composition, energy content and mineral concentrations of okra seeds grown in two locali-ties of Congo Brazzaville were investigated. The paper also reports the physicochemical characteristics of the oil extracted from the seeds. Ash was highest (5.84±0. 2%) in Dolisie okra seeds (DOS) followed by Brazzaville okra seeds (BOS) with a value of 5.52±0.34%. Protein ranged from 25.48±0.57% in...

Author(s): J.M. Nzikou, A. Boussoukou, A. Goma, E. Matouba, J.M. Ouamba, C. Kapseu, M. Parmentier, S. Desobry

December 2006

Time-dependent stability of used engine oil degradation by cultures of Pseudomonas fragi and Achromobacter aerogenes

Pseudomonas fragi and Achromobacter aerogenes isolated from used engine oil polluted soils were grown in minimal salts medium (MSM) supplemented with used engine oil as sole carbon and energy source to evaluate their ability to biodegrade used engine oil. The two organisms utilized 73.3 and 80.0% of the oil with a degradation rate of 0.073 and 0.08 ml/day respectively. The utilization rate of the mixed culture did not...

Author(s): O. O. Adelowo, S. O. Alagbe and A. A. Ayandele

December 2006

Histopathological responses of the gill and liver tissues of Clarias gariepinus fingerlings to herbicide, glyphosate

African catfish, Clarias gariepinus fingerlings were exposed to sub lethal concentrations of herbicide, glyphosate (0, 0.05, 0.1%, v/v) over 42 days period. The gills showed marked alterations in the epithelia in response to glyphosate treatment. There was fusion in adjacent secondary lamella resulting in hyperplasia, with profound oedematous changes, characterised by epithelial detachment. In the liver, the...

Author(s): OLURIN, K.B., OLOJO, E.A.A., MBAKA, G.O. and AKINDELE, A.T.