June 2024

Modifications of growth, mineral uptake, chlorophyll content, osmolyte contents, antioxidant compounds and yield of three varieties of yam (Dioscorea rotundata L.) in saline conditions

A vast area of the world is negatively impacted by salinity which causes loss of crop yields. Modifications in the growth, mineral uptake, chlorophyll content, osmolyte contents, antioxidant compounds and yield of three varieties of yam (Dioscorea rotundata L.) were evaluated in saline conditions, in a greenhouse (0, 50, 100 and 200 mM) and farm (0 and 50 mM). The leaf area, stem height, noose diameter, chlorophyll...

Author(s): Mbondjo Cécile Ndouma, Alphonse Ervé Nouck, Sondi Serge Ekwel, Erica Wiraghan Shang, Momnjoh Edna Kindzeka, Titah Margaret Awah and Victor Desiré Taffouo

June 2024

Assessment and validation of reference genes for qRT-PCR normalization in local cowpea (Vigna unguiculata L.) varieties in response to sterilized and unsterilized soil conditions

Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is widely employed as a mandatory analysis in biological work to analyze and validate gene expression; however, its precision is greatly hampered by the robustness of the internal control genes employed for normalization. In this study, we selected suitable housekeeping genes in leaves of cowpea for normalization of expression analysis when...

Author(s): Frank Kwarteng Amoako, Francis Anti Amoako, Owuraku Amponsah Abu, Melchizedek Asamoah Amponsah, Emmanuel Digooh, Lily Naa Adoley Batsa, Agnes Nimo Bosompem, Clara Ama Adutwumwaa, Belinda Owiah Baiden, Felicity Animah Anarfi, Jeffrey Kankam Boateng, Michael Ackah, Aikins Nyamekye, Obed Adjei and Ruth Prempeh