African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 18 June 2007; 6(12)

June 2007

Biological, ecological and agronomic significance of plant phenolic compounds in rhizosphere of the symbiotic legumes

Phenolics are low molecular compounds ubiquitous in all tissues of higher plants with great significance in plant development. Our understanding of some phenolic compounds in the last few decades has greatly improved. However, their biological, ecological and agronomical significance in the rhizosphere of most symbiotic legumes is much less clear. Further understanding of these biomole-cules will increase our knowledge...

Author(s): Joachim H.J.R. Makoi and Patrick A. Ndakidemi

June 2007

Production and purification of polyclonal antibody against bovine immunoglobulins in rabbits

  Antibodies are important tools in medical researches which have led to many advances in this field. Anti-bovine immunoglobulins and its conjugate with horse radish peroxidase (HRP) is used to diagnose cows’ disease by ELISA or western blotting tests. In this study, the production, purification and horse radish peroxidase (HRP) conjugation of polyclonal IgG against bovine immunoglobulins in rabbits were...

Author(s): Majidi, J, Abdolalizadeh, J, Amirkhiz, MB and Majidi, S

June 2007

a-Tocopherol (vitamin E) attenuates the testicular toxicity associated with experimental cryptorchidism in rats

  Cryptorchidism is the most frequent anatomic anomaly observed in an endocrine gland. It is a well-identified risk factor in infertility and testicular cancer. It is believed that increased generation of free radicals and oxidants play an important role in the resulting testicular damage. The present study investigated the role of a-tocopherol (vitamin E) as an antioxidant in protecting the testis against...

Author(s): Saalu, L. C., Oluyemi, K. A. and Omotuyi, I. O.

June 2007

Rapid high performance liquid chromatographic determination of chlorpropamide in human plasma

Samples were extracted with dichloromethane and the organic layer evaporated to dryness. The residue was dissolved in methanol, and 25 µl aliquot injected onto the column. Tolbutamide was used as the internal standard for chlorpropamide. The UV detector response was linear over the range 0 – 300 µg/ml, with a correlation coefficient of 0.999 and detection limit of 1.30 ng/ml. Within day and between-day...

Author(s): M .T. Bakare Odunola, I. S. Enemali, M. Garba and O. O. Obodozie  

June 2007

Preliminary studies on cytotoxic effect of fungal taxol on cancer cell lines

  Taxol is an important anticancer drug used widely in the clinical field. Some endophytic fungi were isolated from selected medicinal plants and screened for the production of taxol. The effect of cytotoxicity of fungal taxol isolated from fungal endophytes was investigated by apoptosis method. The presence of taxol in the culture filtrate of endophytic fungi was determined by thin layer chromatography. The...

Author(s): V. Gangadevi and J. Muthumary

June 2007

Intragenomic diversity and geographical adaptability of diploid cotton species revealed by cytogenetic studies

  Cotton is one of the most important crops in Iran, and is cultivated in different regions of the country. Gossypium herbaceum is one of the A-genome cottons, which is a potentially important genetic resource for cotton breeding programs. Collecting native cultivars of this species growing in different regions is a vital step in broadening variability of the gene pool. The G. herbaceum is...

Author(s): Alishah Omran, Ahmadikhah Asadollah, and Nasrollanejad Saeid

June 2007

Characterization and monitoring of selected rhizobial strains isolated from tree legumes in Thailand

  Tree legume rhizobia were isolated from five tree legumes; Acacia auriculaformis Cunn., A. mangium Willd., Milletia leucantha Kurz., Pterocarpus indicus Willd., and Xylia xylocarpa Taub. grown in Thailand. Forty four highly effective rhizobial strains were selected on the basis of nitrogenase activity, number of nodules and plant biomass. The selected...

Author(s): Monchai Manassila, Achara Nuntagij, Somsak Kotepong, Nantakorn Boonkerd, and Neung Teaumroong

June 2007

Vigour and behaviour of fifteen citrus varieties against tristeza in the forest zone of Cameroon

  Fifteen varieties or combinations of citrus (lime, grapefruit, lemons, oranges, mandarin and hybrids) were characterized in the forest zone of Cameroon. Characterization was carried out based on the behaviour of these citrus varieties against citrus tristeza virus (CTV). Foliar and cortical symptoms were evaluated. Six years old plants did not show any immune reaction against the disease. Unusual...

Author(s): Ndongo Bekolo, Ambang Zachée, Belibi Messanga Louis, Ngodo Melingui Jean Baptiste and Ongono Yvette

June 2007

A simple and efficient procedure to enhance artemisinin content in Artemisia annua L. by seeding to salinity stress

  Artemisinin is an effective anti-malarial drug extracted from Artemisia annua L. Due to the low content of artemisinin in A. annua, great efforts have been devoted to improve artemisinin production. Here we report a simple and efficient procedure to enhance artemisinin content in A. annua by seeding to salinity stress. Our result shows that artemisinin content in the plant treated...

Author(s): Zhenghao Qian, Ke Gong, Ling Zhang, Jianbing Lv, Fuyuan Jing, Yueyue Wang, Shaobo Guan, Guofeng Wang and Kexuan Tang

June 2007

Effect of substrate supplementation with wheat bran, NPK and urea on Psathyrella atroumbonata Pegler sporophore yield

  Three agricultural by-products (oil palm fruit fibre, banana leaves and sawdust) were supplemented with wheat bran, NPK fertilizer and urea at 5 - 20% (w/w) for wheat bran and 0.1, 0.5, 1.0 and 2% (w/w) for NPK and Urea. The supplemented substrates were used to cultivate Psathyrella atroumbonata. There was sporophore formation in all the substrates supplemented with NPK except at 1% and 2% in oil...

Author(s): Ayodele, S. M. and Okhuoya, J. A.

June 2007

Agronomic performance of locally adapted sweet potato (Ipomoea batatas (L) Lam.) cultivars derived from tissue culture regenerated plants

  Tissue culture techniques have opened a new frontier in agricultural science by addressing food security and agricultural production issues. A study was conducted to compare growth and yield characteristics between the tissue culture regenerated and conventionally propagated sweet potato cultivars. Five locally adapted sweet potato cultivars Mugande, SPK004, Kemb10, Japon...

Author(s): JN Oggema, MG Kinyua, JP Ouma, and JO Owuoche

June 2007

Studies on aerobic biodegradation activities of 2,4-dichlorophenoxyacetic acid by bacteria species isolated from petroleum polluted site

  Bacteria species were screened and monitored for the efficiency of 2,4–dichlorophenoxyacetic acid (2,4-D) degradation from oil degrading laboratory stock with the view to getting the most efficient 2,4-D degraders, to develop an active indigenous bacterial consortium for the bioremediation of 2, 4-D polluted systems in Nigeria. The 2,4-D was utilized as sole source of carbon attaining maximum cell...

Author(s): Igbinosa O. Etinosa, Ajisebutu O. Stella and Okoh I. Anthony

June 2007

Development of amino resin for paint formulation: Copolymerization of methylol urea with polyester

  In our continuous efforts to develop a paint binder from amino resin, a low formaldehyde emission methyol urea-triethanolamine (MUT) composite was synthesized through in situ esterification of formaldehyde with triethanolamine (TEA) and subsequent copolymerization of the synthesized polyester with methylol urea. The effects of addition of TEA on the polymerization process on some physical properties...

Author(s): Osemeahon, S. A. and Barminas, J. T.

June 2007

Biodecolorization and biodegradation of Reactive Blue by Aspergillus sp.

  Aspergillus sp. effectively decolorized Reactive Blue and other structurally different synthetic dyes. Agitation was found to be an important parameter, while glucose (99%),  sucrose (97%) and mannitol (98%) were the best carbon sources for the decolorization. Decolorization was effective in an acidic environment (pH 3). Few chemically different dyes such as Reactive Black (75%), Reactive...

Author(s): Mohandass Ramya , Bhaskar Anusha, S. Kalavathy, and S. Devilaksmi

June 2007

Isolation of antibacterial pentahydroxy flavones from the seeds of Mimusops elengi Linn

  In order to search for antimicrobial phytochemicals, two antibacterial compounds were detected from the seeds of Mimusops elengi Linn. The compounds were extracted by ethyl acetate and purified by column chromatography. Their structures were elucidated through IR, 1H NMR and GC-EL-MS spectral analyses. After characterization, the compounds were identified as...

Author(s): K. M. Hazra, R. N. Roy, S. K. Sen and S. Laskar

June 2007

Biodegradation of penicillin-G wastewater using Phanerochate chrysosporium – An equilibrium and kinetic modeling

  An attempt was made in the present study to find out the biodegradation of the penicillin-G wastewater for the various operational conditions such as, initial substrate concentrations (13000, 10000, 6000, 4000 and 2000 mg of COD/l), agitation, addition of nutrients (glucose and ammonium chloride) and biomass dosages (2, 3, 4 and 5 g) in batch reactor using immobilized cells of Phanerochate...

Author(s): P. Mullai and S. Vishali

June 2007

Glutathione S-transferase (GST) activity as a biomarker in ecological risk assessment of pesticide contaminated environment

  The behaviour and fate of pesticides in the environment will determine their impact on both humans and non-target organisms. Biochemical biomarkers are increasingly used in ecological risk assessment to identify the incidence of exposure to and effects caused by xenobiotics. This study was undertaken to investigate the potential toxic effect of a locally produced insect powder called “Rambo”...

Author(s): Olawale Otitoju and Ikechukwu N.E. Onwurah

June 2007

Genetic diversity and population structure of Ethiopian lentil (Lens culinaris Medikus) landraces as revealed by ISSR marker

  Characterization of genetic resources maintained at genebanks has important implication for future utilization and collection activities. The objective of this study was to assess the level of genetic diversity, genetic structure and genetic distance, and to indirectly estimate the level of gene flow among populations of Ethiopian lentil landraces using intersimple sequence repeat (ISSR) marker. For this...

Author(s): Edossa Fikiru, Kassahun Tesfaye and Endashaw Bekele

June 2007

Isolation, characterization and identification of lactic acid bacteria involved in traditional fermentation of borde, an Ethiopian cereal beverage

Changes in pH, titratable acidity, Enterobacteriaceae, aerobic mesophiles, lactic acid bacteria (LAB) and yeast counts were investigated during borde fermentation. A rapid decrease in pH was associated with accelerated growth rate of LAB and inhibition ofEnterobacteriaceae. Wide diversities of LAB strains were present at early stage ofborde fermentation. The number of species reduced as the...

Author(s): Kebede Abegaz

June 2007

Effect of storage on the brewing properties of tropical hop substitutes

  Tropical hop substitute from utazi (UTZ) Gongronema latifolium, bitter cola (BTC),Garcinia kola, bitter leaf (BTL), Vernonia amygdalina and a blend (1:1.41:2.89) of the three (HSB) respectively, were produced. Stability studies were carried out to predict their suitability for brewing after one to six months storage at 5 ± 1oC and 27 ±1oC, respectively. The...

Author(s): Okoro, Casmir Chukwuemeka and Aina, J. O.