March 2010
The possible effect of the bioaccumulation of disinfectant by-products on crops irrigated with treated wastewater
The shortage of potable water for irrigation of food crops in semi-arid developing countries led to the use of alternative sources of water. Wastewater is gaining importance for productive use in agriculture throughout the world. A widely used and efficient method to reduce the occurrence of waterborne diseases in numerous wastewater plants is water chlorination. In early 1970s, some volatile halogenated organic...
March 2010
Construction of gateway-compatible yeast two-hybrid vectors for high throughput analysis of protein interaction
Yeast two-hybrid system combined with the gateway technology will greatly facilitate the cloning of interested DNA fragment into yeast two-hybrid vectors and therefore increase the efficiency of yeast two-hybrid analysis. In this study, we constructed a pair of Gateway-compatible yeast two-hybrid vectors pBTM116GW and pVP16GW by introducing the gateway cassette (attR1-Cmr-ccdB-attR2) into the multiple cloning...
March 2010
Expression of a natural fusion gene for uracil phosphoribosyltransferase and uridine kinase from rice shows growth retardation by 5-fluorouridine or 5-fluorouracil in Escherichia coli
Uridine kinase (UK) and uracil phosphoribosyltransferase (UPRT) are enzymes in pyrimidine salvage pathway. UK or UPRT were reported as separate genes in bacteria. Amino acid sequence of OsUK/UPRT1 from rice shows homology to bacterial enzymes. Amino-terminal region is similar to UDK with an ATP/GTP-binding motif, whereas the carboxyl-terminal similar to UPP with two binding motifs for a uracil and a PRPP,...
March 2010
Cloning and characterization of a nitrite reductase gene related to somatic embryogenesis in Gossypium hirsutum
A nitrite reductase gene related to somatic embryogenesis was first cloned fromGossypium hirsutum. The cDNA sequence of the gene, named GhNiR, is 2,257 bp in length, with 254 bp of the 5’ untranslated region and 236 bp of the 3’ untranslated region. The open reading frame is 1,767 bp in length, encoding a deduced amino acid sequence of 588 residues with a molecular weight of 65.722 kDa...
March 2010
Assessment of genomic relationship between Oryza sativa and Oryza australinesis
The genomic relationship between Oryza sativa (2n = 24 AA) and Oryza australinesis (2n = 24 EE) has not been established. Genomic relationship between these two species was assessed by using three strategies: genomic in situhybridization (GISH), meiotic chromosome pairing, pollen and spikelet sterility. The hybrid was produced between these two species at the International Rice Research...
March 2010
Rootstock growth and development for increased graft success of mango (Mangifera indica) in the nursery
The graft success of seedlings depends on rootstock size and the skills of grafters. A study was carried out to evaluate the effects of rootstock diameter (at root collar), skills and experience of grafters on mango seedling thinning and grafting. Skilled and unskilled grafters were involved in grafting mango plants for at least three consecutive months. Different rootstock sizes based on root collar diameter of mango...
March 2010
Variation of photosynthetic tolerance of rice cultivars (Oryza sativa L.) to chilling temperature in the light
Forty-two genotypes from the rice germplasm (Oryza sativa L.) were identified under chilling temperature in the light at bud, seedling and booting stages and divided into three basic types; cultivars tolerant to chilling in the light such asjaponica, cultivars sensitive to chilling in the light such as indica and cultivars that have intermediate tolerance to chilling in the light such as hybrid rice...
March 2010
Sugarbeet (Beta vulgaris L.) seed pre-treatment with water and HCl to improve germination
This study was conducted to evaluate the effects of pre-sowing seed treated by soaking in distilled water and two concentrates of hydrochloric Acid (0.03 N HCl and 0.3 N HCl) at three different time ( 2, 4 and 6 h) by factorial arrangement based on completely randomized design with three replications in the University of Mohaghegh, Ardabili (Agronomy Laboratory). For each treatment, 25 uniform seeds were...
March 2010
Modelling the effect of temperature on seed germination in some cucurbits
The prediction of germination percentage (GP) and germination speed (GS) of the seeds for some cucurbits (watermelon, melon, cucumber, summer squash, pumpkin and winter squash) was investigated by mathematical model based on temperature. The model, D = [a - (b x T) + (c x T2)] of Uzun et al. (2001), was adapted to predict both the GP and GS in relation to 12 different temperatures, namely 12, 15, 18, 21, 24, 27, 30, 33,...
March 2010
Influence of temperature, light and plant growth regulators on germination of black pepper (Piper nigrum L.) seeds
Effects of temperature, light and different concentrations of plant growth regulators on germination of Piper nigrum L. seeds was studied under controlled environmental conditions. Black pepper seeds were placed in Petri dishes with filtration papers and the germination and radical development followed during eighteen days periods. The seeds generally germinated within six or seven days. There was no...
March 2010
Activity of Ricinus communis (Euphorbiaceae) against Spodoptera frugiperda (Lepidoptera: Noctuidae)
One of the most studied plant species with insecticidal properties is the castor beanRicinus communis. However, its activity against Spodoptera frugiperda is unclear. Therefore, to determinate the insecticidal and insectistatic activities of methanol, hexane and ethyl acetate extracts of the seeds and leaves of R. communis, castor oil and ricinine were tested at different concentrations...
March 2010
Optimization of medium composition for cis-epoxysuccinate hydrolase production in Escherichia coli by response surface methodology
Response surface methodology was applied to identify and optimize the medium composition for the cis-epoxysuccinate hydrolase production in recombinantEscherichia coli. Plackett-Burman design was used in the first step to evaluate the effects of 8 variables on the enzyme activity. CaCl2, corn steep liquor and lactose were screened as significant factors and their concentrations were further optimized using...
March 2010
Preliminary study on the antioxidant effect of Kigelia africana fruit extract (Bignoniacieae) in male Sprague-Dawley rats
Testicular germ cells as well as epididymal maturing spermatozoa are endowed with enzymatic and non-enzymatic scavenger systems to protect lipid peroxidation damage. A number of pathologies and systemic challenges can lead to an antioxidant/pro-oxidant disequilibrium. With increasing interest in herbal therapies for management of some fertility-related and inflammatory conditions, we investigated the antioxidant effect...
March 2010
Effect of non-nutritional factors on nisin production
When attempting to improve production of nisin, understanding the effect of non-nutritional factors is essential owing to a lack of adequate information about these factors among various investigations. In order to assess some of non-nutritional factors and how they influence the nisin production in batch cultivation, a laboratory scale study was performed. Lactococcus lactis subsp. lactis ATCC 11454...
March 2010
Construction of gateway-compatible yeast two-hybrid vectors for high throughput analysis of protein interaction
Yeast two-hybrid system combined with the gateway technology will greatly facilitate the cloning of interested DNA fragment into yeast two-hybrid vectors and therefore increase the efficiency of yeast two-hybrid analysis. In this study, we constructed a pair of Gateway-compatible yeast two-hybrid vectors pBTM116GW and pVP16GW by introducing the gateway cassette (attR1-Cmr-ccdB-attR2) into the multiple cloning...
March 2010
Substrate molecule enhances the thermostability of a mutant of a family 11 xylanase from Neocallimastix patriciarum
Yeast two-hybrid system combined with the gateway technology will greatly facilitate the cloning of interested DNA fragment into yeast two-hybrid vectors and therefore increase the efficiency of yeast two-hybrid analysis. In this study, we constructed a pair of Gateway-compatible yeast two-hybrid vectors pBTM116GW and pVP16GW by introducing the gateway cassette (attR1-Cmr-ccdB-attR2) into the multiple cloning...
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