African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12184

Table of Content: 1 March 2010; 9(9)

March 2010

The possible effect of the bioaccumulation of disinfectant by-products on crops irrigated with treated wastewater

The shortage of potable water for irrigation of food crops in semi-arid developing countries led to the use of alternative sources of water. Wastewater is gaining importance for productive use in agriculture throughout the world. A widely used and efficient method to reduce the occurrence of waterborne diseases in numerous wastewater plants is water chlorination. In early 1970s, some volatile halogenated organic...

Author(s): Babatunde C. Akande, Patrick A. Ndakidemi, Olalekan Fatoki and James Odendaal  

March 2010

Construction of gateway-compatible yeast two-hybrid vectors for high throughput analysis of protein interaction

Yeast two-hybrid system combined with the gateway technology will greatly facilitate the cloning of interested DNA fragment into yeast two-hybrid vectors and therefore increase the efficiency of yeast two-hybrid analysis. In this study, we constructed a pair of Gateway-compatible yeast two-hybrid vectors pBTM116GW and pVP16GW by introducing the gateway cassette (attR1-Cmr-ccdB-attR2) into the multiple cloning...

Author(s): Tingheng Zhu, Weixia Wang, Hann-lin Wong, Xiao Yang, Kun Wang and Zhifeng Cui

March 2010

Expression of a natural fusion gene for uracil phosphoribosyltransferase and uridine kinase from rice shows growth retardation by 5-fluorouridine or 5-fluorouracil in Escherichia coli

Uridine kinase (UK) and uracil phosphoribosyltransferase (UPRT) are enzymes in pyrimidine salvage pathway. UK or UPRT were reported as separate genes in bacteria. Amino acid sequence of OsUK/UPRT1 from rice shows homology to bacterial enzymes. Amino-terminal region is similar to UDK with an ATP/GTP-binding motif, whereas the carboxyl-terminal similar to UPP with two binding motifs for a uracil and a PRPP,...

Author(s): Md. Shafiqul Islam Sikdar and Jung-Sup Kim

March 2010

Cloning and characterization of a nitrite reductase gene related to somatic embryogenesis in Gossypium hirsutum

A nitrite reductase gene related to somatic embryogenesis was first cloned fromGossypium hirsutum. The cDNA sequence of the gene, named GhNiR, is 2,257 bp in length, with 254 bp of the 5’ untranslated region and 236 bp of the 3’ untranslated region. The open reading frame is 1,767 bp in length, encoding a deduced amino acid sequence of 588 residues with a molecular weight of 65.722 kDa...

Author(s): G. Y. Han#, J. N. Chi#, X. F. Wang, G. Y. Zhang and Z. Y. Ma

March 2010

Assessment of genomic relationship between Oryza sativa and Oryza australinesis

The genomic relationship between Oryza sativa (2n = 24 AA) and Oryza australinesis (2n = 24 EE) has not been established. Genomic relationship between these two species was assessed by using three strategies: genomic in situhybridization (GISH), meiotic chromosome pairing, pollen and spikelet sterility. The hybrid was produced between these two species at the International Rice Research...

Author(s): F. M. Abbasi, H. Ahmad, F. Perveen, Inamullah, M. Sajid and D. S. Brar

March 2010

Rootstock growth and development for increased graft success of mango (Mangifera indica) in the nursery

The graft success of seedlings depends on rootstock size and the skills of grafters. A study was carried out to evaluate the effects of rootstock diameter (at root collar), skills and experience of grafters on mango seedling thinning and grafting. Skilled and unskilled grafters were involved in grafting mango plants for at least three consecutive months. Different rootstock sizes based on root collar diameter of mango...

Author(s): Simon A. Mng’omba, Festus K. Akinnifesi, Gudeta Sileshi and Oluyede C. Ajayi

March 2010

Variation of photosynthetic tolerance of rice cultivars (Oryza sativa L.) to chilling temperature in the light

Forty-two genotypes from the rice germplasm (Oryza sativa L.) were identified under chilling temperature in the light at bud, seedling and booting stages and divided into three basic types; cultivars tolerant to chilling in the light such asjaponica, cultivars sensitive to chilling in the light such as indica and cultivars that have intermediate tolerance to chilling in the light such as hybrid rice...

Author(s): Xia Li, Kun Cao, Chao Wang, Zhi-wei Sun and Lina Yan

March 2010

Sugarbeet (Beta vulgaris L.) seed pre-treatment with water and HCl to improve germination

This study was conducted to evaluate the effects of pre-sowing seed treated by soaking in distilled water and two concentrates of hydrochloric Acid (0.03 N HCl and 0.3 N HCl) at three different time ( 2, 4 and 6 h) by factorial arrangement based on completely randomized design with three replications in the University of Mohaghegh, Ardabili (Agronomy Laboratory). For each treatment, 25 uniform seeds were...

Author(s): Maralian Habib

March 2010

Modelling the effect of temperature on seed germination in some cucurbits

The prediction of germination percentage (GP) and germination speed (GS) of the seeds for some cucurbits (watermelon, melon, cucumber, summer squash, pumpkin and winter squash) was investigated by mathematical model based on temperature. The model, D = [a - (b x T) + (c x T2)] of Uzun et al. (2001), was adapted to predict both the GP and GS in relation to 12 different temperatures, namely 12, 15, 18, 21, 24, 27, 30, 33,...

Author(s): Ertan Sait Kurtar

March 2010

Influence of temperature, light and plant growth regulators on germination of black pepper (Piper nigrum L.) seeds

Effects of temperature, light and different concentrations of plant growth regulators on germination of Piper nigrum L. seeds was studied under controlled environmental conditions. Black pepper seeds were placed in Petri dishes with filtration papers and the germination and radical development followed during eighteen days periods. The seeds generally germinated within six or seven days. There was no...

Author(s): Zhigang Li, Aiqin Liu, Huasong Wu, Lehe Tan, Yuzhou Long, Yafeng Gou, Shiwei Sun and Liwei Sang  

March 2010

Activity of Ricinus communis (Euphorbiaceae) against Spodoptera frugiperda (Lepidoptera: Noctuidae)

One of the most studied plant species with insecticidal properties is the castor beanRicinus communis. However, its activity against Spodoptera frugiperda is unclear. Therefore, to determinate the insecticidal and insectistatic activities of methanol, hexane and ethyl acetate extracts of the seeds and leaves of R. communis, castor oil and ricinine were tested at different concentrations...

Author(s): M. A. Ramos-López, S. Perez-G, C. Rodríguez-Hernández, P. Guevara-Fefer and Miguel A. Zavala-Sánchez  

March 2010

Optimization of medium composition for cis-epoxysuccinate hydrolase production in Escherichia coli by response surface methodology

Response surface methodology was applied to identify and optimize the medium composition for the cis-epoxysuccinate hydrolase production in recombinantEscherichia coli. Plackett-Burman design was used in the first step to evaluate the effects of 8 variables on the enzyme activity. CaCl2, corn steep liquor and lactose were screened as significant factors and their concentrations were further optimized using...

Author(s): Hai-feng Pan[], Wen-na Bao, Zhi-peng Xie and Jian-guo Zhang,

March 2010

Preliminary study on the antioxidant effect of Kigelia africana fruit extract (Bignoniacieae) in male Sprague-Dawley rats

Testicular germ cells as well as epididymal maturing spermatozoa are endowed with enzymatic and non-enzymatic scavenger systems to protect lipid peroxidation damage. A number of pathologies and systemic challenges can lead to an antioxidant/pro-oxidant disequilibrium. With increasing interest in herbal therapies for management of some fertility-related and inflammatory conditions, we investigated the antioxidant effect...

Author(s): Azu, O. O., Duru, F. I. O., Osinubi, A. A, Noronha, C. C., Elesha, S. O. and Okanlawon A. O.

March 2010

Effect of non-nutritional factors on nisin production

When attempting to improve production of nisin, understanding the effect of non-nutritional factors is essential owing to a lack of adequate information about these factors among various investigations. In order to assess some of non-nutritional factors and how they influence the nisin production in batch cultivation, a laboratory scale study was performed. Lactococcus lactis subsp. lactis ATCC 11454...

Author(s): Say-yed Hesameddin Tafreshi, Saeed Mirdamadi, Dariush Norouzian, Shohreh Khatami and Soroush Sardari  

March 2010

Construction of gateway-compatible yeast two-hybrid vectors for high throughput analysis of protein interaction

Yeast two-hybrid system combined with the gateway technology will greatly facilitate the cloning of interested DNA fragment into yeast two-hybrid vectors and therefore increase the efficiency of yeast two-hybrid analysis. In this study, we constructed a pair of Gateway-compatible yeast two-hybrid vectors pBTM116GW and pVP16GW by introducing the gateway cassette (attR1-Cmr-ccdB-attR2) into the multiple cloning...

Author(s): Tingheng Zhu, Weixia Wang, Hann-lin Wong, Xiao Yang, Kun Wang and Zhifeng Cui

March 2010

Substrate molecule enhances the thermostability of a mutant of a family 11 xylanase from Neocallimastix patriciarum

Yeast two-hybrid system combined with the gateway technology will greatly facilitate the cloning of interested DNA fragment into yeast two-hybrid vectors and therefore increase the efficiency of yeast two-hybrid analysis. In this study, we constructed a pair of Gateway-compatible yeast two-hybrid vectors pBTM116GW and pVP16GW by introducing the gateway cassette (attR1-Cmr-ccdB-attR2) into the multiple cloning...

Author(s): Tingheng Zhu, Weixia Wang, Hann-lin Wong, Xiao Yang, Kun Wang and Zhifeng Cui