African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Table of Content: 21 June, 2017; 16(25)

June 2017

Kinetic characterization and molecular modeling of trehalose-6-phosphate phosphatase from Anopheles gambiae and expressed in Pichia pastoris

Trehalose-6-phosphate phosphatase (TPP) is one of the primary enzymes involved in the synthesis of trehalose, the main sugar found in insect hemolymph. In the present study, we report for the first time heterologous expression in Pichia pastoris, characterization and homology modeling of TPP from Anopheles gambiae mosquito. Purified TPP recombinant exhibited a molecular weight of approximately 36 kDa with optimum pH of...

Author(s): Marcos Cézar Fernandes Pessoa, Érika Izumi, Sandra Patrícia Zanotto and Spartaco Astolfi-Filho

June 2017

Leaf conditioning of Brazilian Cerrado species for DNA microextraction

Proper conditioning of leaf tissues in collection expeditions can affect the quantity and quality of DNA in the extraction process. The aim of this work was to define a method of preserving foliar tissue suitable for obtaining DNA from Brazilian Cerrado trees. Young leaves of species (Mangaba and Baru) were collected and conditioned in five different treatments during a period of six days. Genomic DNA was obtained using...

Author(s): Luiza Gabriela Fulgêncio de Lima, Caio César de Oliveira Pereira, Camila Gracyelle de Carvalho Lemes, Anderson Rodrigo da Silva, Juliana Oliveira da Silva, Dieferson da Costa Estrela, Guilherme Malafaia and Ivandilson Pessoa Pinto de Menezes

June 2017

Effect of Moringa oleifera leaf extract on the haematological and serum biochemistry of rabbits reared in a semi-humid environment

This research is aimed at improving the productive and pathological picture of rabbits reared in a semi-humid environment using Moringa oleifera leaf extract (MOLE). Twenty four mixed- breed rabbits, having an average weight of 700g, were used in this study. They were randomly divided into four equal treatments (6 rabbits each) and gavaged with various concentrations of MOLE. Treatment 1 (control) was given 0ml MOLE/kg...

Author(s): Ojo, O. A. and Adetoyi, S. A.

June 2017

Heavy metal contamination in soils from a municipal landfill, surrounded by banana plantation in the eastern flank of Mount Cameroon

Municipal solid waste generated in our cities, with an unprecedented population growth, has resulted in degrading environmental quality, thus a major problem for policy makers. The extent of Pb, Cu, Zn, Cd, Hg and Zn contamination in soils of the studied area, where vegetables were grown, using metal contamination factor (CF) and pollution load index were assessed. The concentrations of heavy metals studied were in the...

Author(s): Fonge, B. A., Nkoleka, E. N., Asong, F. Z., Ajonina, S. A. and Che, V. B.

June 2017

Improvement of color and increase in anthocyanin content of ‘Niagara Rosada’ grapes with application of abscisic acid

This study aimed to assess the application effect of abscisic acid (S-ABA) on color, and total anthocyanin and phenolic compound contents of the ‘Niagara Rosada’ grapes. The experiment was carried out in a commercial vineyard located in Jales, SP, Brazil, during 2014/2015. The randomized block design was used with four treatments and five replications. The treatments consisted of applying different...

Author(s): Marco Antonio Tecchio, Francisco José Domingues Neto, Adilson Pimentel Junior, Marlon Jocimar Rodrigues da Silva, Sérgio Ruffo Roberto and Ronny Clayton Smarsi

June 2017

DEXseq and Cuffdiff approaches weighing differential spliced genes exons modulation in estrogen receptor β (Erβ) breast cancer cells

While the alternative transcription and splicing mechanisms have long been known for some genes like oncogenes, their prevalence in almost all multi-exon genes has been recently realized with the increasing application of high-throughput experimental methods, named Next Generation RNA Sequencing (NGS). Henceforth, understanding the regulation of these processes in comparisons between cell types and cancer requests,...

Author(s): Noel Dougba Dago, Nafan Diarrassouba, Inza Jesus Fofana, Jean-Luc Aboya Moroh, Hermann-Désiré Lallié, Didier Martial Yao Saraka, Oléfongo Dagnogo, Souleymane Silué, Armel Herve Nwabo Kamdje, Lamine Baba-Moussa and Alessandro Weisz

June 2017

Production potential and chemical composition of elephant grass (Pennisetum purpureum Schum.) at different ages for energy purposes

The elephant grass has gained prominence as one of the main forage species used for biomass production. Therefore, the aim of this study was to identify elephant grass genotypes with high energy biomass production potential by evaluating morpho-agronomic and biomass quality. The following traits were evaluated in this study: dry matter yield (DMY), percentage of whole-plant dry matter (%DM), percentage of neutral...

Author(s): Ana Kesia Faria Vidal, Tatiane da Costa Barbé, Rogério Figueiredo Daher, Janeo Eustáquio Almeida Filho, Roberta Samara Nunes de Lima, Rafael Souza Freitas, Drieli Aparecida Rossi, Érik da Silva Oliveira, Bruna Rafaela da Silva Menezes, Geovana Cremonini Entringer, Wanessa Francesconi Stida Peixoto and Sabrina Cassaro