August 2018

Development and validation of a high performance liquid chromatography-diode array detection (HPLC-DAD) method for the quantification of rutin and isoquercetin in Morus nigra L. (Moraceae)

Morus nigra L. is widely used by the population of the São Francisco Valley (Brazil) for the treatment of several diseases such as hypertension, diabetes, arthritis etc. The objective of this study was to develop and validate an analytical method that is capable of identifing and quantifing flavonoids in the ethanolic crude extract (Mn-EtOH) and its ethyl acetate phase (Mn-AcOEt) obtained from M. nigra leaves...

Author(s): Pedrita Alves Sampaio, Grasielly Rocha Souza, Pedro Guilherme Sousa de Sá, Nathália Andrezza Carvalho de Souza, José Marcos Teixeira de Alencar Filho, Pedro José Rolim-Neto, Jackson Roberto Guedes da Silva Almeida and Larissa Araújo Rolim  

August 2018

Effect of spices on the microbiological quality of Hibiscus sabdariffa (zobo) drink and molecular characterization of the associated spoilage organisms

Hibiscus sabdariffa drink (zobo) was preserved with three concentrations of cloves (Syzygium aromaticum), ginger (Zingiber officinale) and mixture of cloves and ginger, respectively while the control had no spice. The bottled H. sabdariffa drink was stored at 28 ± 2°C for 16 days. The total bacteria and fungi counts were determined every 48 h during storage. Bacterial and fungal growths were not detected in...

Author(s): Adeoye, B. K., Oyewole, O. B. , Idowu, M. A., Obadina, A. O., Ani, I. F. and Ngozi, E. O.    

August 2018

Camwood (Baphia nitida) alcoholic extract: A suitable counter stain for haematoxylin in the demonstration of liver and kidney histomorphology

The counter staining ability of Camwood (Baphia nitida) alcoholic extract and its electrostatic compatibility with haematoxylin was determined. One gram of locally sourced red paste was subjected to simple extraction in 100 ml of 70 and 95% alcohol, respectively. Primary staining of buffered formalin fixed, paraffin embedded liver and kidney tissues was done with haematoxylin followed by counter staining with extract...

Author(s): Omowumi, B. J. , Benard, S. A., Afolabi, O. O., Fowotade, A. A. and Olutunde, O. A.  

August 2018

Genetic diversity among elite chickpea (Cicer arietinum L.) varieties of Ethiopia based on inter simple sequence repeat markers

The inter simple sequence repeat (ISSR) marker was used in this study for genetic fingerprinting and identification of released chickpea varieties and for the determination of the genetic relationships among these cultivars. A total of 19 released chickpea varieties were subjected to ISSR analysis with the objective of evaluating the genetic diversity among the cultivars. A total of 20 ISSR primers were initially...

Author(s): Zerihun Jida, Shiferaw Alemu and Destaw Mullualem