African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12476

Table of Content: 22 October, 2014; 13(43)

October 2014

An efficient hairy root culture system for Withania somnifera (L.) Dunal

Withania somnifera is an important aromatic medicinal plant and possesses wide array of pharmacological properties. In the present investigation, an improved version of hairy root culture system was developed by optimizing various transformation parameters such as type of explant, concentration of acetosyringone, Agrobacterium types and co-cultivation period. Between the leaf and cotyledon explants and two Agrobacterium...

Author(s): Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi and Markandan Manickavasagam

October 2014

Plasmid profile of multi antibiotic resistant staphylococcus aureus isolated from diabetic wounds from patients at Nsukka, South-eastern, Nigeria

Multi-drug resistant bacterial strains evolving worldwide has created a great public health problem that needs urgent attention; as such bacteria show resistance to the drug of choice for treatment as well as being resistant to newer and last line antibiotics. In this study, the antibiotic susceptibility, multi antibiotic resistance (MAR) index and plasmid profile of MDR Staphylococcus aureus isolated from diabetic...

Author(s): Badger-Emeka, L. I., Emeka, P. M. and Dibua, U. M. E.

October 2014

Effect of starter cultures on microbial and physicochemical parameters of a dry fermented poultry meat sausage

The aim of this work was to study the effect of Staphylococcus xylosus and Lactobacillus plantarum on microbial and physicochemical characteristics of a dry fermented poultry meat sausage. The microbial results show that lactic acid bacteria (LAB) and coagulase negative staphylococci (CNS) represent the two predominant microflora in all stages of ripening. The number of enterobacteria decreased significantly in sausages...

Author(s): Sabrine El ADAB, Ines ESSID, and Mnasser HASSOUNA

October 2014

Towards the development of sweet potato-based couscous for human consumption in Benin

Sweet potato processing and consumption patterns are very limited in Benin. The present study aimed to suggest a new utilization of the crop as food. Roots from a white flesh variety were processed into flour and later into couscous. This couscous was steam-cooked following the same procedure as a wheat-based couscous purchased in market. The foods obtained were submitted to panellists’ appreciation. Although...

Author(s): Charlotte Fifamè KPOMASSE, Sègla Wilfrid PADONOU, Jean Louis AHOUNOU and Paul HOUSSOU

October 2014

Antiproliferative and antibacterial activity evaluation of red microalgae Rhodosorus marinus

Antiproliferative activity of three extracts obtained from red microalgae Rhodosorus marinus was evaluated against cervical (HeLa), colon (HCT 116), lung (A549), prostate (22Rv-1) and breast (HCC38 and MDA-MB-231) cancer cell lines. Antibacterial activity of these extracts was also tested against Salmonella choleraesuis, Listeria monocytogenes and Staphylococcus aureus. All extracts were obtained from lyophilized...

Author(s): Garcia-Galaz, Alfonso, Gutiérrez-Millán, Luis E., Valdés-Covarrubias, Miguel A., Acedo-Félix, Evelia, Burgos-Hernández, Armando, López-Torres, Marco A. and María Guadalupe Burboa Zazueta

October 2014

Pathogenicity and proteome production of Isaria fumosorosea (=Paecilomyces Fumosoroseus) (WISE) isolates against lemon butterfly, Papilio demoleus (Papilionidae: lepidoptera)

The pathogenic potential and catalytic triad conserved amino acids of the isolates Isaria fumosorosea (=Paecilomyces fumosoroseus) (Ifr1 and Ifr2) in response to Papilio demoleus was analysed. The isolates showed its potential in killing P. demoleus causing mortality of 72.23 and 61.90% at the end of 8 days with 108 spores ml-1 concentrations. The enzyme assays (higher proteolytic and chitinolytic activity) also showed...

Author(s): Vinayaga Moorthi, P. C. Balasubramanian, Pasco B. Avery, Selvarani, S., T. Kubendran, T. Rathinakumar and A. Najitha Banu

October 2014

Evaluation of oral vaccination of village chickens against newcastle disease with I-2 vaccine coated parboiled cracked maize in Enderta District, Tigray, Ethiopia

The study was conducted to assess the suitability of soaked parboiled cracked maize as a carrier of I-2 vaccine for oral immunization of village chickens. Chickens were vaccinated once via ocular route and orally with cracked maize at the second and fifth weeks of the experiment. Post vaccination serum was collected 4, 7, 9 and 11 weeks of the experiment and haemagglutination inhibition test was done to evaluate the...

Author(s): Kassaw Amssalu Tadesse, Biruk Mekonnen Woldie, Yohannes Tekle Asfaw, Habtamu Tassew Tarekegn, Edlu Jorga and Esayas Gelaye Leykun