African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12498

Table of Content: 24 August 2011; 10(47)

August 2011

Economic importance of Gypsophila L., Ankyropetalum Fenzl and Saponaria L. (Caryophyllaceae) taxa of Turkey

Gypsophila L. is the third biggest genus of Caryophyllaceae family in Turkey. The genus has 55 species in the country. 33 species are endemics and total number of the taxa is 58. Ankyropetalum Fenzl is a small genus with 3 species and 1 of them is endemic. Saponaria L. has 20 taxa of 18 species and 10 taxa of them are endemics to Turkey. Turkey is the gene center of all the genera. All of...

Author(s): Mustafa Korkmaz and Hasan Özçelik

August 2011

miRNAs: Small but deadly

microRNAs (miRNAs) are unique class of global gene regulators identified both in plants and animals. They can reduce protein levels of their target genes with a minor impact on the target genes mRNA. Levels of some miRNAs are found altered in cancers, so we might expect these regulatory molecules to be involved in the development of different carcinomas. The differential expression of certain miRNAs in various tumors...

Author(s): Raisa Bano, Sahar Fazal, Mohammad Haroon Khan and Hamid Rashid

August 2011

Linkage and mapping analyses of the no glue egg gene Ng in the silkworm (Bombyx mori L.) using simple sequence repeats (SSR) markers

In the silkworm, Bombyx mori, no glue egg is mainly controlled by Ng (No glue)gene, which is located on the 12th chromosome. Owning to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progenies were used for linkage analysis and mapping of the Ng gene based on the simple sequence repeats (SSR)linkage map using silkworm...

Author(s): Xiao-ming Zhao#, Guo-qing Wei#, Chao-liang Liu, Chang-rui Zou  and Bao-jian Zhu   #Both authors contributed equally to this work.

August 2011

Genetic diversity of taraxacum germplasm revealed by sequence-related amplified polymorphism (SRAP) analysis

Sequence-related amplified polymorphism (SRAP) and morphological markers were employed to determine the genetic diversity and relations among 11 population of taraxacum in northeast of China. Data on 34 morphological traits were collected and analyzed. A total of 795 polymorphic SRAP’s bands were scored with 20 combinations of primers. The genetic relationships analyzed with un-weighed pair-group method with...

Author(s): Zhigang Wu,  Wei Ning, Yaosheng Wang, Shuangshuang Lv, Yabo Zhaoand Xin Zhao

August 2011

Identification of a microsatellite marker linked to the fertility-restoring gene for a polima cytoplasmic male-sterile line in Brassica napus L.

Polima cytoplasmic male sterility (pol CMS) has been widely used for exploiting heterosis in rapeseed breeding. The dominant restorer gene of pol CMS (Rfp) is found in the nucleus and is a key component of hybrid production by achieving F1 progeny with complete fertility restoration. To identify the molecular markers associated with the Rfp gene, a near isogenic line (NIL) population of...

Author(s): Yun Li,  Zhi Liu#, Qiang Cai, Guangsheng Yang, Qingbiao He and Pingwu Liu’

August 2011

Assessment of genetic diversity for some Iraqi date palms (Phoenix dactylifera L.) using amplified fragment length polymorphisms (AFLP) markers

Amplified fragment length polymorphisms (AFLP) were used to evaluate the genetic diversity between 18 date palm (Phoenix dactylifera L.) varieties (11 females and 7 males) collected from the center of Iraq. Six primer pairs were applied to detect polymorphism between varieties. A total of 83 polymorphic AFLP fragments were detected with an average of 13.8 polymorphic fragments/primer combination. Genetic distance...

Author(s): Khierallah, H., Bader, S., Baum, M. and Hamwieh, A.

August 2011

Activity of acetolactate synthase (ALS) of redroot pigweed in relation to imazethapyr application

Seed of weed species redroot pigweed for which there exist possibility of resistance occurrence were collected from different localities in Autonomous Province of Vojvodina, the northern part of the Republic of Serbia (Krivaja, Kikinda, Vrbas and Kačarevo). Studies on herbicide resistance were performed in the period of 2003 to 2009. Biological studies included whole plant studies on plants grown in net-house and...

Author(s): Meseldzija Maja and Konstantinovic Branko

August 2011

Controlling bruchid pests of stored cowpea seeds with dried leaves of Artemisia annua and two other common botanicals

Insecticidal activities of dried Artemisia annua L. leaves were evaluated against bruchid (Callosobruchus maculatus F.) pests in comparison with those ofAzadirachta indica, Ocimum gratissimum and a conventional grain storage insecticide, Actellic® 2% dust. Each treatment was added to a mixture of 250 g cowpea seeds and 25 adult bruchids and laid out in a completely randomized design...

Author(s): Ebiamadon Andi Brisibe, Sophia Eloho Adugbo, Uduak Ekanem, Fraideh Brisibe and Glyn Mara Figueira

August 2011

Drying characteristics of bay laurel (Laurus nobilis L.) fruits in a convective hot-air dryer

In this study, drying characteristics of bay laurel (Laurus nobilis L.) fruits wereinvestigated in a laboratory scale hot-air dryer at air temperature in a range of 70 to 100°C. Moisture transfer from the test samples was described by applying the Fick’s diffusion model and the effective diffusivity was calculated. Temperature dependence of the effective diffusivity was described by the...

Author(s): Y. Benal Yurtlu

August 2011

Preliminary study of the contribution of native legumes to the nitrogen economy of natural grasslands

The need to discover suitable native legumes of high nitrogen fixing ability was pinpointed. Potential native legumes growing in the wild in Nsukka, Nigeria were identified and the seeds were collected. Desmodium ramisissimon was found to be the common legume species in the University area and was used for germination and nodulation studies. D. ramisissimon showed good potentiality with regards to...

Author(s): Onyeonagu, C. C. and Asiegbu, J. E.

August 2011

Acibenzolar-S-methyl induces lettuce resistance against Xanthomonas campestris pv. vitians

Acibenzolar-S-methyl (Benzo [1,2,3] thiadiazole-7-carbothioic acid-S-methyl ester, ASM; Bion 50 WG) was found to be more protective for lettuce against bacterial speck disease caused by Xanthomonas campestris pv. vitians. The experiments were conducted under controlled climatic conditions in the greenhouse, and induction of defense mechanism using ASM treatment against challenged inoculation...

Author(s): Fahri Yigit

August 2011

Efficacy of preharvest spraying with Pichia guilliermondii on postharvest decay and quality of cherry tomato fruit during storage

This study investigated the effects of preharvest spraying with Pichia guilliermondiion postharvest natural decay of cherry tomato fruit, and evaluated the quality attributes during storage. Preharvest spraying with P. guilliermondii significantlydecreased the decay index. Moreover, the efficacy of this biological control was positively correlated with spraying frequency....

Author(s): Yan Zhao, Ruolan Wang, Kang Tu and Kunlun Liu

August 2011

Biofertilization of micropropagated Agave tequilana: Effect on plant growth and production of hydrolytic enzymes

Three beneficial bacterial strains [Gluconoacetobacter diazotrophicus (Pal5), the diazotrophs (11B) and Pachaz (008)] and an arbuscular mycorrhizal fungus [Glomus intraradices (AMF)] were evaluated for their ability to enhance plant growth and the production of hydrolytic enzymes in micropropagated Agave tequilanaWeber var. Blue. Results show that the growth of the agave plants and the production of...

Author(s): Sonia Ruiz, Lourdes Adriano, Isidro Ovando, Cuauhtemoc Navarro and Miguel Salvador

August 2011

Surface runoff and phosphorus (P) loss from bamboo (Phyllostachys pubescens) forest ecosystem in southeast China

The effect of different fertilization treatments on runoff and nutrient losses under field conditions was investigated through setting runoff plots in bamboo (Phyllostachys pubescens) forests in a catchment of Taihu Lake. The results showed that, the runoff loss reached 356, 361 and 342 m3 /hm2, while the soil particle loss reached 393, 392 and 442 kg/hm2, respectively, in the period from June...

Author(s): Qi-Chun Zhang, Ying-ying Liang, Imran Haider Shamsi, Guang-huo Wang, Li-ping Lou and Nazim Hussain

August 2011

Effect of exogenous methyl jasmonate on growth, gas exchange and chlorophyll contents of soybean subjected to drought

Drought is considered as one of the major constraints to crop production worldwide. Methyl jasmonate (MJ) is a plant-signaling molecule that elicits a wide variety of plant responses ranging from morphological to molecular level. A pot-culture study was undertaken to investigate the possible role of MJ-treatment on growth, gas exchange and chlorophyll contents in soybean (Glycine max L. Merrill.) plants...

Author(s): Shakeel Ahmad Anjum, Xiao-yu Xie, Muhammad Farooq, Long-chang Wang, Lan-lan Xue, Muhammad Shahbaz and Jalaladeen Salhab

August 2011

Morphological responses of forage sorghums to salinity and irrigation frequency

Water stress occurs when the demand for water exceeds the available amount during a certain period or when poor quality water restricts its use. In particular, water stress is associated with low availability of water, as well as osmotic effects associated with salinity. The response of forage sorghum [Sorghum bicolor (L.) Moench] varieties to salinity and irrigation frequency were studied from December 2007 to...

Author(s): Saberi A. R., Siti Aishah H., R. A. Halim and Zaharah A. R.

August 2011

Production of β-glucanase enzyme from Penicillium oxalicum and Penicillium citrinum

Two Penicillium species namely, Penicillium oxalicum and Penicillium citrinumcultivated by solid surface fermentation method using rice bran homogenized with 0.5% (w/v) (NH4)2SO4 solution as nitrogen source and Whatman no. 1 filter paper (WFP1) as substrate for β-glucanase enzyme production medium were found to show a dense growth. Studies on the enzyme, using soluble...

Author(s): Doughari, James Hamuel

August 2011

Toxicity of the insect growth regulator lufenuron on the entomopathogenic fungus Metarhizium anisopliae (Metschnikoff) Sorokin assessed by conidia germination speed parameter

Metarhizium anisopliae has been considered a promising alternative with low environmental impacts for the biological control of a variety of insect-pests. Another alternative is the use of biological pesticides such as insect growth regulators, including lufenuron. An assessment of the potential impact of fungicides on M. anisopliae is of critical importance to integrated pest management, permitting the...

Author(s): Monique Meiko Takeda de Almeida Alves, Ravely Casarotti Orlandelli, Daniela Andressa Lino Lourenço and João Alencar Pamphile

August 2011

Investigating the sourdough potential for enhance microbiological shelf life and roasty aroma of traditional Lavash bread

In this study, potential effects of sourdough on microbiological shelf life and roasty aroma of traditional Lavash bread were studied. For investigating the biopreservation effect of sourdough, after intentional contamination of flour with hygiene indicator microorganisms that cause mouldiness and ropiness, survival curves of these microorganisms versus different sourdough fermentation times and temperatures, at...

Author(s): Seyed Ali Mortazavi and Alireza Sadeghi

August 2011

Investigating the sourdough potential for enhance microbiological shelf life and roasty aroma of traditional Lavash bread

In this study, potential effects of sourdough on microbiological shelf life and roasty aroma of traditional Lavash bread were studied. For investigating the biopreservation effect of sourdough, after intentional contamination of flour with hygiene indicator microorganisms that cause mouldiness and ropiness, survival curves of these microorganisms versus different sourdough fermentation times and temperatures, at...

Author(s): Seyed Ali Mortazavi and Alireza Sadeghi

August 2011

Effect of polyamines on thermotolerance and membrane stability of soybean seedling

The role of polyamines in the heat-shock protection of soybean seedling by application of exogenous polyamines (putrescine, spermidine and spermine) and their biosynthetic inhibitors involving CHA (cyclohexylamine) and DFMO (D, L α-diflouromethylornithine) was investigated. Application of polyamines, as a pre-treatment at 28°C for 2 h prior to heat-shock (45°C for 2 h), enhanced the recovery growth of both...

Author(s): R. Amooaghaie and S. Moghym

August 2011

Melanoidin decolorization mechanism and some properties of Issatchenkia orientalis No.SF9-246

Issatchenkia orientalis No. SF9-246 cell showed both melanoidin (MP) adsorption and degradation abilities and its MP-adsorption yield was reduced by acclimatization with medium containing MP. The living cell showed the MP-adsorption ability of 15%; higher than the autoclaved cell (dead cell). The MP-adsorption ability of the deteriorated cell (MP-adsorbed cell) was recovered by washing with 0.1 mol...

Author(s): Suntud Sirianuntapiboon and Tusanee Tondee

August 2011

Antibacterial activity of various fractions of ethyl acetate extract from the desert truffle, Tirmania pinoyi, preliminarily analyzed by gas chromatography-mass spectrometry (GC-MS)

The antibacterial activity of various fractions of ethyl acetate extract isolated from edible fungi, Tirmania pinoyi (Maire) Malençon, growing in Algeria, was investigated. Extraction was done by the Soxhlet and the fractions obtained were purified with silica-gel column. Two fractions of ethyl acetate extract were tested against the bacteria Escherichia coli ATCC 25922, Bacillus...

Author(s): Dib-Bellahouel, S. and Fortas, Z.

August 2011

Anthelmintic efficacy of cashew (Anarcadium occidentale L.) on in vitro susceptibility of the ova and larvae of Haemonchus contortus

The use of plants for the treatment of human and animal diseases continues to rise although there are few studies providing proof of these effects. Among them is theAnacardium occidentale L., popularly known as cashew. In vitro egg hatch and larval development and viability assays was conducted to determine possible direct anthelmintic effect of acetone extract and fractions of A....

Author(s): I. O. Ademola, and J. N. Eloff

August 2011

A novel approach for very early pregnancy diagnosis in swine by anti-early pregnancy factor (EPF) antiserum blocking enzyme-linked immunosorbent assay (ELISA)

Early pregnancy factor (EPF) is essential for the initiation and maintenance of pregnancy. Early pregnancy factor activity monitoring has been reported to be the effective method for very early pregnancy diagnosis. In this study, three BALB/c mice were immunized with the synthetic peptide segment corresponding to the amino acid sequence 36 to 55 of EPF (IG20) for...

Author(s): Kai Quan, Xingxu Zhao, Changxing Zhang, Qiuliang Xu, Hongfang Wei, Junjie Hu and Yong Zhang