African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genetic diversity of taraxacum germplasm revealed by sequence-related amplified polymorphism (SRAP) analysis

Zhigang Wu1,2,  Wei Ning1*, Yaosheng Wang3, Shuangshuang Lv4, Yabo Zhao1and Xin Zhao1
1College of Horticulture, Shenyang Agricultural University, Shenyang, 110886, China. 2Floricultural Research Institute, Liaoning Academy of Agricultural Sciences. 3Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Højbakkegård Allé 13, DK-2630 Taastrup, Denmark. 4Institute of Analysis, Shenyang Agricultural University, Shenyang 110886, China.
Email: [email protected]

  •  Accepted: 22 June 2011
  •  Published: 24 August 2011

Abstract

Sequence-related amplified polymorphism (SRAP) and morphological markers were employed to determine the genetic diversity and relations among 11 population of taraxacum in northeast of China. Data on 34 morphological traits were collected and analyzed. A total of 795 polymorphic SRAP’s bands were scored with 20 combinations of primers. The genetic relationships analyzed with un-weighed pair-group method with arithmetic mean (UPMGA) showed that 11 population of taraxacum were grouped into three clusters. The results reveal by SRAP molecular markers were consistent with those based on the agronomic traits, suggesting that SRAP markers could be used in the taxonomic analysis of taraxacum germplasm.

 

Key words: Taraxacum, sequence-related amplified polymorphism (SRAP), genetic diversity, un-weighed pair-group method with arithmetic mean (UPMGA).

Abbreviation

SRAPSequence-related amplified polymorphism