African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12293

Table of Content: 24 June, 2015; 14(25)

June 2015

In vitro microtuberization of Black Zira (Bunium persicum Boiss.)

Bunium persicum or Black Zira is one of the endangered species in the land of Persia. The main purpose of this study was to investigate microtuberization of B persicumin in order to use in germplasm storage and commercial production. Seeds of B. persicum were used as explant. Different culture media (MS, ½MS and B5) along with different concentrations of jasmonic acid (JA) (0, 2 and 5) were used individually as...

Author(s): Hossein Mardani, Seyed Mahdi Ziaratnia, Majid Azizi, Han Phyo Aung, Kwame Sarpong Appiah and Yoshiharu Fujii

June 2015

Effects of aqueous extracts of Mucuna sloanei (Fabaceae) seed on haematological parameters of albino rats (Rattus novergicus)

Mucuna sloanei is a legume used as a soup thickener by communities in some parts of Africa countries. The effect of aqueous seed extract of M. sloanei on the haematological profile of normal albino rats was investigated for 28 days using standard methods. The results show no overall dose dependent significant difference (p > 0.05) in the serum levels of the white blood cell count, red blood cell count and its indices...

Author(s): Ejere, Vincent C., Ugwu, Godwin C., Chukwuka, Christian O. and Nnamonu, Emmanuel I.

June 2015

Investigation of stress tolerance of endoglucanases of the cellulosomes of Clostridium cellulolyticum to ethanol

Current energy and environmental challenges are driving the use of cellulosic materials for biofuel production. A major obstacle in this pursuit is poor ethanol tolerance among cellulolytic Clostridium species. The objective of this work was to establish a potential upper boundary of ethanol tolerance for the cellulosome itself. The hydrolytic function of crude cellulosome extracts from Clostridium cellulolyticum on...

Author(s): Ugochukwu Anieto

June 2015

Fenthion induced toxicity and histopathological changes in gill tissue of freshwater African catfish, Clarias gariepinus (Burchell, 1822)

Fenthion is an organophosphate pesticide commonly used in agriculture and public health for the control of insect pests. The present study investigated the toxic effect of fenthion and the histopathological alterations in the gill tissue of African catfish Clarias gariepinus. The 96 h LC50 value of fenthion in C. gariepinus determined by probit analysis was found to be 39.97 mg/L. Fish exposed to different fenthion...

Author(s): Peace Onas Somdare, Christopher Didigwu Nwani, Alfreda O. Nwadinigwe, Jacinta Chinedu Nwani, Gregory Ejike Odo, Ogechi Nnabuchi Ugbor, Juliana A Ukonze and Ada Bridget Chidi Ezeibe

June 2015

Cellular biomarker responses of bagrid catfish, Chrysichthys nigrodigitatus in a contaminated coastal ecosystem

An assessment of the pollution status of Agboyi creek, a water body associated with various anthropogenic activities was carried out in order to determine responses induced in Catfishes, Chrysichthys nigrodigitatus inhabiting it. Cellular biomarkers of stress including the antioxidative stress enzyme, catalase (CAT), lipid peroxidation measured as thiobarbituric acid reactive substance (TBARS), as well as serum enzymes...

Author(s): Ejimadu C. Uzoma, Chukwu L. Obinna and Amaeze H. Nnamdi

June 2015

Expression of GFSKLYFamide-like neuropeptide in the digestive system of the sea cucumber Holothuria scabra (Echinodermata)

Neuropeptides are key mediators of physiological processes in animals and a considerable amount of information has been accumulated on their diversity and functions across phyla. However, progress in echinoderm neurobiology has been much slower than others. The sea cucumber Holothuria scabra is an economically important tropical echinoderm species in which a neuropeptide is yet to be identified. This immunohistochemical...

Author(s): Abayomi Ajayi, and Boonsirm Withyachumnarnkul,

June 2015

Compound serum and hemin free medium for cultivation of Leishmania tarentolae: A recombinant protein expression system

Serum free cultivation of Leishmania is cost-effective and improves large scale production of well-defined parasite material. Moreover, the production of recombinant pharmaceutical proteins requires cultivation of the host in a culture medium free of animal materials, so several culture media for Leishmania tarentolae expression system have been introduced. Some investigations have established the development of a...

Author(s): Jalali Akram, Bandehpour Mojgan, and Kazemi Bahram,

June 2015

Genetic diversity in Jatropha species from different regions of Brazil based on morphological characters and inters-simple sequence repeat (ISSR) molecular markers

Jatropha belonging to Euphorbiaceae family has around 170 species distributed throughout tropical semi-arid regions of Africa and the Americas. Some of its species include Jatropha curcas L. (Physic Nut), Jatropha pohliana Müll.Arg. (Brazilian Purging Nut) and Jatropha gossypiifolia L. (Black Physic Nut). Phenotypic and genetic studies of a population are important for plant improvements, helping in the...

Author(s): Mariana Silva Rosa Pazeto, Sandra Helena Unêda-Trevisoli, Aretha Arcenio Pimentel Corrêa, Viviane Formice Vianna, Daniel Carvalho Leite and Antônio Orlando Di Mauro