African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

In vitro microtuberization of Black Zira (Bunium persicum Boiss.)

Hossein Mardani*
  • Hossein Mardani*
  • Laboratory of International Agro-Biological Resources and Allelopathy, Tokyo University of Agriculture and Technology, Japan.
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Seyed Mahdi Ziaratnia
  • Seyed Mahdi Ziaratnia
  • Department of Food Technology, Research Institute of Food Science and Technology, Mashhad, Mashhad, Iran.
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Majid Azizi
  • Majid Azizi
  • Department of Horticulture, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
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Han Phyo Aung
  • Han Phyo Aung
  • Laboratory of International Agro-Biological Resources and Allelopathy, Tokyo University of Agriculture and Technology, Japan.
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Kwame Sarpong Appiah
  • Kwame Sarpong Appiah
  • Department of International Environmental and Agricultural Science in Tokyo University of Agriculture and Technology, Japan.
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Yoshiharu Fujii
  • Yoshiharu Fujii
  • Tokyo University of Agriculture and Technology, Division of International Environmental Agriculture, Laboratory of International Agro-Biological Resources and Allelopathy, Japan.
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  •  Received: 15 January 2015
  •  Accepted: 17 June 2015
  •  Published: 23 June 2015

Abstract

Bunium persicum or Black Zira is one of the endangered species in the land of Persia. The main purpose of this study was to investigate microtuberization of B persicumin in order to use in germplasm storage and commercial production. Seeds of B. persicum were used as explant. Different culture media (MS, ½MS and B5) along with different concentrations of jasmonic acid (JA) (0, 2 and 5) were used individually as basal media and also in combination with two different temperatures (15 and 20°C) to develop appropriate media for microtuberization. Moreover, propagated microtubers were then vernalized and acclimatized in order to transfer to greenhouse. The results revealed that by increasing in concentration of JA, weight and length of microtubers increased significantly. MS medium seemed to be the most effective basal medium for this plant. In contrary, this study indicated that MS medium and 5 mM JA were the most suitable combination for in vitro culture establishment and short-term maintenance of tested B. persicum. Also, 15°C showed significant effect on increasing the weight of microtubers.

 

Key words: Microtuberization, Bunium persicum, jasmonic acid, temperature, medium.