African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12209

Table of Content: 24 May, 2017; 16(21)

May 2017

Antibiotic susceptibility pattern of Pseudomonas aeruginosa expressing blaGES and blaPER genes in two different hospitals

Pseudomonas aeruginosa is intrinsically resistant to several antimicrobial drugs classes. Various extended-spectrum beta lactamases (ESBL) types have been found in P. aeruginosa such as Pseudomonas extended resistance (PER) and Guiana extended-spectrum (GES) enzymes. The study aimed to evaluate the susceptibility of the ESBL producing P. aeruginosa strains that express blaGES and blaPER genes to commonly used...

Author(s): Omar B. Ahmed and Atif H. Asghar

May 2017

Genetic diversity and population structure of Ethiopian finger millet (Eleusine coracana (L.) Gaertn) genotypes using inter simple sequence repeat (ISSR) markers

Eleusine coracana is an annual allotetraploid (2n=4x=36) that belongs to grass family, Poaceae. This research aimed to investigate level of genetic diversity of 80 accessions using ISSR markers. DNA was extracted from a bulk of three plants per accession using a modified CTAB method. Six ISSR primers amplified a total of 45 clear and reproducible bands. The total genetic diversity (H) and Shannon’s diversity...

Author(s): Haftom Brhane, Teklehaimanot Haileselassie and Kassahun Tesfaye

May 2017

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

A Gram-positive, rod-shaped, spore-forming haloalkaliphilic bacterium designated as NA7 was isolated from the surface of a Helianthemum nummularium root sample obtained from Wadi Natrun in Egypt. Sequence analysis of the 16S rRNA gene revealed a Bacillus haloalkaliphilius strain as the closest match with 99% identity. In a shake flask culture containing 10% NaCl, adjusted to pH 10 and incubated at 37°C, the isolated...

Author(s): Walid Ahmed Lotfy, Neveen Mohamed Abd-El-Karim, Ebaa Ebrahim El-Sharouny and Ehab Ragheb El-Helow

May 2017

Diversity of Aspergillus isolates and selection of an isolate with high β-fructofuranosidase activity that is native to the Peruvian coast

In order to obtain a native isolate with high β-fructofuranosidase activity (FFase), a preliminary selection of 44 isolates, predominantly formed by Aspergillus niger (72%), was performed among  167 isolates of Aspergillus native to the Peruvian coast (Casa grande, Cartavio, Paramonga and Tacama). In addition, genetic diversity was studied using molecular markers, Inter Simple Sequence Repeats (ISSR). The...

Author(s): Boris Gutarra Castillo, Marina Díez-Municio, Susan Canchaya Garcia and Gaby Calixto Zacarias, Juan Cisneros de la Cruz and F Javier Moreno

May 2017

Myracrondruon urundeuva Allemão: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

Caatinga biome features species with high therapeutic potential such as Aroeira do Sertão (Myracrodruon urundeuva Allemão) which has long history in folk medicine. This study aimed to chemically characterize the secondary metabolites of hydroethanolic extract and hexane, chloroform, acetate, hydroethanolic fractions from Myracrodruon urundeuva (Allemão) possibly responsible antioxidant and...

Author(s): André Luiz Lima Menezes Santos, José Davi Prado Lima, Clivia Rolemberg Andrade, Deisylaine Maria Santos, Antonio Santos Dias, Pietra Alexia Lima Santos, Rafaela Karolina Vianna Nunes, José Evaldo Rodrigues Menezes Filho, José Nilson Andrade Santos, Carla Maria Lins Vasconcellos, Andrea Yu Kwan Shan, Brancilene Santos Araujo and Charles Santos Estevam

May 2017

Chromosomal and plasmid mediated degradation of crude oil by Bacillus coagulans, Citrobacter koseri and Serratia ficaria isolated from the soil

In this work, the degradative abilities of some bacteria isolated from the soil were studied. Five distinct isolates were subjected to substrate susceptibility test to determine their potential degradative abilities in the presence of different hydrocarbons. The best three crude oil degraders were selected and their identities were further established using API technique. Furthermore, the extent of each degradative...

Author(s): Fagbemi O. Kehinde and Sanusi A. Isaac