African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Full Length Research Paper

Myracrondruon urundeuva Allemão: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

Andre Luiz Lima Menezes Santos
  • Andre Luiz Lima Menezes Santos
  • Biochemistry and Natural Products Chemistry Laboratory, Physiology Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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Jose Davi Prado Lima
  • Jose Davi Prado Lima
  • Biochemistry and Natural Products Chemistry Laboratory, Physiology Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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Clivia Rolemberg Andrade
  • Clivia Rolemberg Andrade
  • Biotechnology and Natural Products Chemistry Laboratory, Physiology Department, Federal Universityof Sergipe, Aracaju, SE, Brasil.
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Deisylaine Maria Santos
  • Deisylaine Maria Santos
  • Biotechnology and Natural Products Chemistry Laboratory, Physiology Department, Federal Universityof Sergipe, Aracaju, SE, Brasil.
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Antonio Santos Dias
  • Antonio Santos Dias
  • Biochemistry and Natural Products Chemistry Laboratory, Physiology Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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Pietra Alexia Lima Santos
  • Pietra Alexia Lima Santos
  • Biochemistry and Natural Products Chemistry Laboratory, Physiology Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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Rafaela Karolina Vianna Nunes
  • Rafaela Karolina Vianna Nunes
  • Biochemistry and Natural Products Chemistry Laboratory, Physiology Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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Jose Evaldo Rodrigues Menezes Filho
  • Jose Evaldo Rodrigues Menezes Filho
  • Heart Biophysics Laboratory Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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Jose Nilson Andrade Santos
  • Jose Nilson Andrade Santos
  • Heart Biophysics Laboratory Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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Carla Maria Lins Vasconcellos
  • Carla Maria Lins Vasconcellos
  • Heart Biophysics Laboratory Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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Andrea Yu Kwan Shan
  • Andrea Yu Kwan Shan
  • Biochemistry and Natural Products Chemistry Laboratory, Physiology Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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Brancilene Santos Araujo
  • Brancilene Santos Araujo
  • Biochemistry and Natural Products Chemistry Laboratory, Physiology Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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Charles Santos Estevam
  • Charles Santos Estevam
  • Biochemistry and Natural Products Chemistry Laboratory, Physiology Department, Federal University of Sergipe, Aracaju, SE, Brazil.
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  •  Received: 13 January 2017
  •  Accepted: 27 March 2017
  •  Published: 24 May 2017

Abstract

Caatinga biome features species with high therapeutic potential such as Aroeira do Sertão (Myracrodruon urundeuva Allemão) which has long history in folk medicine. This study aimed to chemically characterize the secondary metabolites of hydroethanolic extract and hexane, chloroform, acetate, hydroethanolic fractions from Myracrodruon urundeuva (Allemão) possibly responsible antioxidant and antimicrobial activity, besides to evaluate the inotropic effect of crude extract in the left atrium of Cavia porcellus. Phytochemical composition was evaluated by spectrophotometry and high-performance liquid chromatography (HPLC). Antioxidant activity was assessed by 2,2-diphenyl-1-picryl hidrazyl (DPPH•) scavenging and reactive substances to thiobarbituric acid methods. Inotropic effect was evaluated using the isolated organ model. Data were expressed as mean ± SEM and differences determined by ANOVA followed by Tukey test. Phytochemical screening and the HPLC confirmed the presence of flavonoids, terpenes, tannins. Acetate and Hydroethanolic extract showed inhibition of DPPH values of 81.56 and 93.28% respectively. On the other hand, TBARS reduced in extract were 64.13% in a concentration of 150 µg/mL. Hydroethanolic extract and acetate fraction with concentrations of 50 and 100 mg/mL showed inhibition zones between 11.00 and 20.50 mm suggesting antimicrobial activity against Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae bacteria and Candida albicans. The isolated atrium model demonstrated that the extract acts non-competitively antagonizing muscle calcium currents, decreasing the force of contraction by about 60%. Flavonoids, terpenes and tannins are responsible for M. urundeuva (Allemão) biological effects.

 

Key words: Myracrondruon urundeuva, chemical composition, antioxidant activity, antimicrobial activity, inotropic effect.