October 2012
Genomic and proteomic analysis with dynamically growing self organising tree (DGSOT) for measuring clinical outcomes of cancer
Genomics and proteomics microarray technologies are used for analysing molecular and cellular expressions of cancer. This creates a challenge for analysis and interpretation of the data generated as it is produced in large volumes. The current review describes a combined system for genetic, molecular interpretation and analysis of genomics and proteomics technologies that offers a wide range of interpreted...
October 2012
Genetic diversity analysis of the medicinal herb Plantago ovata (Forsk.)
Plantago ovata (Forsk.) (2n = 8) used as laxative, emollient and demulcent, has great commercial and medicinal importance. With India being the largest producer in the world there is still a lack of defined varieties of the species and no coordinated breeding efforts are being made. In the present study, we report the phylogenetic analysis of the crop for its utilization in future breeding programs for...
October 2012
Diversity of Morinda citrifolia L. in Andaman and Nicobar Islands (India) assessed through morphological and DNA markers
Morinda citrifolia L. is an important plant species for traditional medicine systems which also has immense scope in pharmaceutical industry. The aim of this study was to assess the genetic diversity of the 33 accessions using morphological traits and molecular markers. The accessions showed great variation among morphological parameters. The germplasm showed great variation for fruit weight which ranged...
October 2012
Regeneration of okra (Abelmoschus esculentus L.) via apical shoot culture system
The present study was undertaken to evaluate the most suitable concentration of growth regulators for regeneration of okra (Abelmoschus esculentus L. Monech) via apical shoot culture system. The study of apical shoot culture system was found effective for regeneration of apical shoots. The okra (A. esculentus L. Monech) N-550 line evolved at R&D, Nirmal Seeds Pvt. Ltd., was...
October 2012
Effect of soil salinity on the growth, amino acids and ion contents of rice transgenic lines
Rice seedling of different transgenic lines (T-99, T-112, T-115 and T-121) were grown in sand culture with salt concentration of 0, 50, 100 and 150 mM to determine the effect of salinity on growth, amino acid, and ion contents. It was observed that all the lines could tolerate concentration of up to 50 mM of salt solution. The lines (T-99, T-112 and T-115) were more salt tolerant even at a concentration of...
October 2012
Cry3A δ-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop
Bacillus thuringiensis Cry3A gene was redesigned for high expression in Norwegian spruce and the sequence was slightly modified to allow for simple N- and C- terminal deletions and domain II loop 1 exchange for synthetic oligos. Modified Cry3A toxins from 13 variants of the synthetic gene were expressed inEscherichia coli BL21 and their toxicity on spruce bark beetle larvae was tested using spruce...
October 2012
Anti-viral activity of Hydnellum concrescens, a medicinal mushroom
Trafficking of viral glycoprotein to the surface of infected-cells results in syncytium formation in Newcastle disease virus (NDV)-infected baby hamster kidney (BHK)cells. Hydnellum concrescens, known as a medicinal mushroom, inhibited not only syncytium formation, but also trafficking of glycoprotein, hemagglutinin-neuramidase (HN) to the cell-surface. Viral glycoprotein is processed within...
October 2012
Effect of Artemisia alba L. extract against ethinylestradiol induced genotoxic damage in cultured human lymphocytes
In the present study, the antigenotoxic effect of Artemisia herba-alba was evaluated against the genotoxic effect induced by 10 μM of ethinylestradiol in the presence of metabolic activation using mitotic index (MI), chromosomal aberrations, sister chromatid exchanges and replication index (RI) as parameters. The treatment of 10 μM of ethinylestradiol along with 102, 212.5, 315 and 417μg/mL...
October 2012
Early detection of gray mold in grape using conventional and molecular methods
Botrytis cinerea affects grape quality and yield, and can be difficult to manage due in part to non-symptomatic, quiescent infection in berry development. The aim of this study was to develop a dual system for the detection, isolation and quantification of B. cinerea. After three days of samples replication on the modified selective medium (mKERS), the results showed a significant infection effect...
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