African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Effect of Artemisia alba L. extract against ethinylestradiol induced genotoxic damage in cultured human lymphocytes

  Haytham M. Daradka1 and Nasser Moh`d Yousef Alshibly2*  
  1Biology Department, Faculty of Science, Jerash University, P.O. Box 311, Jerash - Postal Code 26150. Jordan 2Department of Biology, Faculty of Sciences and Arts, King Abdulaziz University,Khulais, Saudi Arabia.
Email: [email protected]

  •  Accepted: 27 July 2012
  •  Published: 25 October 2012

Abstract

 

In the present study, the antigenotoxic effect of Artemisia herba-alba was evaluated against the genotoxic effect induced by 10 μM of ethinylestradiol in the presence of metabolic activation using mitotic index (MI), chromosomal aberrations, sister chromatid exchanges and replication index (RI) as parameters. The treatment of 10 μM of ethinylestradiol along with 102, 212.5, 315 and 417μg/mL of Artemisia albaextract in culture medium resulted in a significant dose dependent decrease in the genotoxic effects induced by the treatment of 10 μM of ethinylestradiol. The results of the present study suggest that the plant extract per se does not have genotoxic potential, but can modulate the genotoxicity of ethinylestradiol in cultured human lymphocytes.

 

Key words: Artemisia alba, Methyltestosterone, ethinylestradiol, human lymphocytes, genotoxicity, natural products, chromosomal aberrations, sister chromatid exchanges.

Abbreviation

Abbreviations: MI, Mitotic index; SCE, sister-chromatid exchanges; RI, replication index.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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