African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Table of Content: 29 April 2015; 14(17)

April 2015

Genetic diversity analysis of mustard (Brassica spp.) germplasm using molecular marker for selection of short duration genotypes

Molecular characterization of 16 mustard (Brassica spp.) genotypes by using 12 RAPD markers revealed that three primers GLA-11, OPB-04 and OPD-02 showed good technical resolution and sufficient variations among different genotypes. A total of 40 RAPD bands were scored of which 38 (94.87%) polymorphic amplification products were obtained. Besides, the primer OPD-02 amplified maximum number of polymorphic bands (100.00%)...

Author(s): Md. Shahin Iqbal, Islam Hamim, Md. Shamiul Haque and Ujjal Kumar Nath

April 2015

Isolation and identification of native lower fungi for polyunsaturated fatty acid (PUFA) production in Thailand, and the effect of carbon and nitrogen sources on growth and production

This research focuses on isolation and identification of polyunsaturated fatty acid (PUFA) producing fungi from natural sources of Thailand, followed by experimental designs for carbon and nitrogen utilizations of the potential strains. The results show that 8 and 16 isolates of lower fungi from freshwater and wet-fallen leaves, respectively, could produce PUFAs. Among these isolates, the NR06 that was isolated from...

Author(s): Supavadee Deelai, Satinee Suetrong, Somchit Damrianant, Panida Unagul and Niramol Sakkayawong

April 2015

Molecular genetic diversity study of Lepidium sativum population from Ethiopia as revealed by inter simple sequence repeat (ISSR) markers

Lepidium sativum L. (family Brassicaceae), is an underutilized medicinal plant with worldwide distribution. In Ethiopia, L. sativum occurs in all regions and agro-ecologies at different altitudinal ranges. The study was conducted to assess the genetic diversity of L. sativum population from Ethiopia using inter simple sequence repeat (ISSR) marker. Molecular data generated from ISSR bands recorded was used for computing...

Author(s): Said Mohammed and Kassahun Tesfaye

April 2015

Effects of selected culture media on mycellial growth of oyster mushroom (Pleurotus ostreatus)

The effect of selected culture media on mycelial growth of Pleurotus ostreatus was studied on six different culture media: three synthetic [malt extact agar (MEA), potato dextrose agar (PDA) and Saubroud dextrose agar (SDA)] and three formulated from locally available agricultural wastes [corncob (CCEA), palm waste (PWEA) and cassava peels (CPEA)]. The tissue culture of the mushroom was done on MEA which was used as the...

Author(s): Chuku, S. B., Nwachukwu, E. O. and Stanley, H. O.

April 2015

The population structure of wild sorghum species in agro-ecological zones of Western Kenya

There is need to understand the genetic structure of wild sorghums that grow alongside cultivated traditional sorghum varieties in order to assess the potential effect of crop genes in wild populations. In this study, 175 wild sorghum samples were collected from 13 agroecological zones (AEZs) from three counties in Western Kenya and genotyped using microsattelite markers. Crop alleles were observed in wild sorghum...

Author(s): Titus O. Magomere, Eliud K. Ngugi, Silas D. Obukosia , Eunice Mutitu and Solomon I. Shibairo

April 2015

Insights on predominant edible bamboo shoot proteins

Juvenile bamboo shoots have become a US$ 18 billion industry and have lured interest worldwide for its nutritive value and health enhancing properties; making it a suitable candidate for food security. Quantitative analysis revealed that juvenile bamboo shoots are a good source of proteins. However, there is no qualitative analysis describing unique proteins present in the edible bamboo shoots. In order to provide the...

Author(s): Sayanika Devi Waikhom, Louis Bengyella,, Pranab Roy and Narayan Chandra Talukdar

April 2015

Activity of the fungus Pleurotus ostreatus and of its proteases on Panagrellus sp. larvae

Biological control has been shown to be one of the possible biotechnological applications of fungi and their proteases. The objective of this study was to evaluate the nematicidal activity of the fungus Pleurotus ostreatus and its proteases on Panagrellus sp. larvae. Proteolytic activity of P. ostreatus (PLO 06) was measured and characterized at different pHs, temperatures and in the presence of a inhibitor (PMSF)....

Author(s): Hugo Leonardo André Genier, Filippe Elias de Freitas Soares, José Humberto de Queiroz, Angélica de Souza Gouveia, Jackson Victor Araújoa, Fabio Ribeiro Braga, Iara Rebouças Pinheiro and Maria Catarina Megumi Kasuya

April 2015

Enhanced recombinant human blood coagulation factor VIII expression in the milk of goats using replication-defective adenovirus

High-level expression of recombinant human blood coagulation factor in milk of farm animals at a large scale provides a powerful tool for protein production. However, a bottleneck in recent protein synthesis technologies is the high cost of current transgenic livestock system. Here, we report a simple, rapid, and low-cost protein production method based on a replication-defective adenoviral vector system. The...

Author(s): Zengsheng Han, Lin Wang, Kun Li, Jian Li, Hongwei Zhao, Zhiwei Liu, Wenguang Cao and Qingwang Li,

April 2015

Effect of the somatic cell count on physicochemical components of milk from crossbred cows

The aim of the present work was to evaluate the effect of somatic cell count on the composition of milk in individual samples from crossbred cows at the Rio Verde municipality. Data from 2,730 individual samples of milk from crossbred cows were used. The samples were collected between February and April 2012 and analyzed at the Milk Quality Laboratory (Laboratório de Qualidade do Leite) in the Food Research...

Author(s): Bruna Ariel Dias Guariglia, Priscila Alonso dos Santos, Liomar de Souza Araújo, Cristiane Isabo Giovannini, Rodrigo Balduíno Soares Neves, Edmar Soares Nicolau and Marco Antônio Pereira da Silva