African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12267

Table of Content: 3 August, 2016; 15(31)

August 2016

Assessment of feeding value of vegetable-carried pineapple fruit wastes to Red Sokoto goats in Ogbomoso, Oyo State of Nigeria

This study compared the sun-drying characteristics of five blends each (w/w; 1:1, 1:1.5, 1:2, 1:2.5, 1:3) of wheat offal-carried pineapple waste (WO:PW) and brewers’ dried grains-carried pineapple waste (BDG:PW), assessed the blends for their nutrient contents and the feeding value of the optimum blends with Red Sokoto (RS) goats. Moisture contents of all the blends were reduced to between 10.95 - 14.38% and 11.73...

Author(s): Vincent Olajide ASAOLU, Rachael Temitope BINUOMOTE and Oyeniyi Sunmiboye OYELAMI

August 2016

Isolation and characterization of two malathion-degrading Pseudomonas sp. in Egypt

Pseudomonas aeruginosa and Pseudomonas mendocina degrading malathion were studied. Morphological, biochemical and 16S rRNA genes for bacterial identification were selected. Biodegradation of some organophosphorus compounds with the 2 bacterial isolates was determined by high performance liquid chromatography (HPLC). P. aeruginosa strain completely removed diazinon, malathion and fenitrothion, but not chlorpyrifos within...

Author(s): Amal E. Saafan, Ahmed F. Azmy, Magdy A. Amin, Shabaan H. Ahmed and Tamer M. Essam

August 2016

Analysis of the impact of domestication of Warburgia ugandensis (Sprague) on its genetic diversity based on amplified fragment length polymorphism

Warburgia ugandensis Sprague (Canellaceae) occurs in East and Central Africa and is an important multipurpose tree species. Over-exploitation of natural forests for medicinal purposes and clearance for farming threaten the species survival. Cultivation of the tree species would ensure sustainable medicinal source and its conservation. However, on-farm genetic diversity of the species is currently unknown. The genetic...

Author(s): Nkatha Gacheri, Bramwel W Wanjala, Ramni Jamnadass and Alice Muchugi,

August 2016

Optimization, purification and characterization of recombinant L-asparaginase II in Escherichia coli

We studied optimal L-asparaginase sequence from GenBank accession number X12746 encoding for L-asparaginase from Erwinia chrysanthemi NCPPB1125. The expression level of recombinant L-asparaginase was determined as 78% of the total proteins. The purified L-asparaginase had a molecular mass of 37 kDa with specific activity of 312.8 U/mg. Kinetic parameters, Km, Vmax, Kcat and Kcat/Km of purified enzyme were found to be...

Author(s): Trang Thi Hien Nguyen, Cuong Tien Nguyen, Thanh Sy Le Nguyen and Tuyen Thi Do

August 2016

Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa

Banana Germplasm Collection serves as a source of useful genes for banana breeding. However, insufficient and/or inaccurate information on the ploidy level of the germplasm renders its utilization in breeding difficult. The objective of this study was to determine and validate the ploidy level of 120 banana accessions in the ex situ germplasm collection centre for the East and Central Africa, located in Mbarara, Uganda....

Author(s): Deborah Karamura, Robooni Tumuhimbise, Sedrach Muhangi, Moses Nyine, Micheal Pillay, Reuben Ssali Tendo, David Talengera, Priver Namanya, Jerome Kubiriba and Eldad Karamura