African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Analysis of the impact of domestication of Warburgia ugandensis (Sprague) on its genetic diversity based on amplified fragment length polymorphism

Nkatha Gacheri
  • Nkatha Gacheri
  • Kenyatta University, P.O. Box 43844-00100, Nairobi, Kenya
  • Google Scholar
Bramwel W Wanjala
  • Bramwel W Wanjala
  • Kenya Agricultural Research Institute, Biotechnology and Biodiversity, P.O Box 14733 – 00800, Nairobi, Kenya.
  • Google Scholar
Ramni Jamnadass
  • Ramni Jamnadass
  • World Agroforestry Centre, P.O. Box 30677-00100, Nairobi, Kenya.
  • Google Scholar
Alice Muchugi
  • Alice Muchugi
  • Kenyatta University, P.O. Box 43844-00100, Nairobi, Kenya
  • Google Scholar


  •  Received: 14 February 2016
  •  Accepted: 11 July 2016
  •  Published: 03 August 2016

Abstract

Warburgia ugandensis Sprague (Canellaceae) occurs in East and Central Africa and is an important multipurpose tree species. Over-exploitation of natural forests for medicinal purposes and clearance for farming threaten the species survival. Cultivation of the tree species would ensure sustainable medicinal source and its conservation. However, on-farm genetic diversity of the species is currently unknown. The genetic diversity of the on-farm W. ugandensis populations and their proximate natural populations were analyzed using the amplified fragment length polymorphism (AFLP). Four primer combinations produced a total of 223 polymorphic bands. Both the natural and on-farm populations had high genetic diversity ranging from H = 0.2892 to H = 0.1278. Principal co-ordinates analysis and dendrogram separated the ten populations into two major groups corresponding to Kenyan and Tanzanian populations, respectively. Ugandan populations were shared between the two major groups; this is probably because Uganda is believed to be the centre of diversity for W. ugandensis. Close genetic relationships between the on-farm and their proximate natural population were revealed. Analysis of molecular variance (AMOVA) revealed that a total of 54% AFLP variation resided within populations with 46% reside among populations. The high genetic diversity of W. ugandensis on-farm populations could be useful in germplasm collection and conservation strategies.

 

Key words: Warburgia ugandensis, amplified fragment length polymorphism (AFLP), domestication, genetic diversity, on-farm, natural.