African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12497

Table of Content: 3 May 2012; 11(36)

May 2012

On the scale-neutrality of transgenic seeds: Micro-level access, impacts and implications for further research

  For transgenic seeds to contribute to agricultural development and food security, transaction cost-related factors, gender issues, institutional constraints and smallholder farmers’ preferences will have to be addressed. In the light of the prevailing controversies and debates, this paper partially reviews the transgenic seeds’ impact on the literature to assess their scale neutrality and identify...

Author(s): Edilegnaw Z. Wale

May 2012

Overview on the efforts to generate transgenic chicken

  After 25 years, the search for the avian cell that can be cultured indefinitely, genetically modified, and clonally derived while retaining its ability to enter the germ line has ended. Van de Lavoir et al. have defined the conditions for culture and genetic modification of primordial germ cells (PGCs) and showed that these cells are transmitted at high rates through the germline. The advent of this...

Author(s): Esmaeilzadeh Mahdi and Kazemzadeh Fariba

May 2012

The relationship estimates amongst milk yield and milk composition characteristics of Bunaji and Friesian × Bunaji cows

  The milk yield of 24 Bunaji and 26 cross breed (Friesian × Bunaji) cows were analyzed for milk yield characteristics [Initial milk yield (IY), peak week yield (PWK), peak yield (PY), end of lactation yield (ENLY), lactation length (LL) and total milk yield (TMY)] and milk composition [fat, crude protein (CP), solid-not- fat (SNF), total solid (TS) and ash]. The relationship within and between the milk...

Author(s): Alphonsus, C. and Essien, I. C.

May 2012

Response to selection for shell length in the noble scallop, Chlamys nobilis (Reeve)

  The noble scallop, Chlamys nobilis is one of the most economically important fishery and aquaculture species in the southern coast of China. In the present study, we conducted a one generation mass selection for shell length in a cultured noble scallop stock with a selection intensity of 1.732, to examine their response to selection. The results show that the selected line grew faster than the...

Author(s): Jianyong Liu and Zhigang Liu

May 2012

Molecular cloning of human dendritic cell associated lectin-1 (DECTIN-1) isoform genes, expression and localization as a green fluorescent protein (GFP) fusion in caco-2 cell line

  The human Dectin-1 molecule known as a β-glucan receptor is an immune cell surface receptor implicated in the immunological defense against fungal and other pathogens. Dectin-1 is a type II transmemebrane receptor with a single extracellular carbohydrate recognition domain (CRD), a stalk region which separates the CRD from the membrane and an immunoreceptor tyrosine activation motif in its...

Author(s): Esayas Gelaye, Jan Roosien and Arjen Schots

May 2012

Inter-specific relationships among two Tunisian Thymus taxa: Thymus capitatus Hoffm. et Link. and Thymus algeriensis Boiss. et Reut. using molecular markers

  Genetic relationships between two sympatric species Thymus capitatus Hoffm. et Link. and Thymus algeriensis Boiss. et Reut. (Thymus hirtus Willd. subsp. algeriensisBoiss. et Reut.) were assessed using random amplified polymorphic DNA (RAPD) markers. Eighteen natural populations from different geographical and bioclimatic zones were evaluated. The seven selected primers generated...

Author(s):    Imen BEN EL HADJ ALI, Arbi GUETAT and Mohamed BOUSSAID   

May 2012

Application of microsatellite polymorphisms to study the diversity in seed oil content and fatty acid composition in Nigerian sesame (Sesamum indicum L.) accessions

  Improving oil yield and a healthy fatty acid profile is a major breeding objective in sesame. In this study, the variability in simple sequence repeat motifs were used to study the diversity in 30 Nigerian sesame accessions in relation to the analyzed variation in oil content and fatty acid composition as a fundamental step towards its genetic improvement. Sesame seed oil content ranged from 40 to - 55 %...

Author(s): Friday Nwalo Nweke, Benjamin Ewa Ubi and  Karl Kunert 

May 2012

Biochemical and histological changes in Dendrobium sonia-28 protocorm-like bodies (PLBs) at various low temperatures and under mineral oil layer

  The study was conducted on the storage of Dendrobium sonia-28 protocorm-like bodies (PLBs) at various temperatures (5, 10, 15, 20, and 25°C) and under mineral oil layer. Biochemical analyses were conducted on the PLBs by determining thechlorophyll, total soluble protein and peroxidase activities. In addition, histological analyses of cell structure, rate of survival and shoot growth after...

Author(s): Robiah Omar, Bhavani Balakrishnan, Advina Lizah Julkifle and Sreeramanan Subramaniam

May 2012

Standardization of different media for in vitro pollen germination of almond and evaluation of the germination capacity of stored pollen

  Almond is an important nut crop which, mostly for fruit set, needs the pollination of flowers followed by fertilization. Therefore, pollen viability and its germination capability are essential. To optimize the pollen culture medium of almond and standardization of the best medium, the present study was carried out with 48 different culture media containing different compositions, including diverse...

Author(s): Ali Imani

May 2012

Effects of zinc and nitrogen application on agronomic traits and qualitative characteristic of sunflower in saline condition

  This present study was performed at the Department of Soil Science of Karaj Branch, during the two seasons of 2008 to 2009 and 2009 to 2010. The experimental design aimed to study the response of sunflower to different nitrogen and zinc fertilizer levels under two doses of salinity stress. The obtained results from the two years showed that nitrogen increased seed yield while there was no...

Author(s): Mohammad Taher Nezami and Ghazaleh Vafaei

May 2012

Thermoluminescence (TL) analysis for otoliths of the wild carps (cyprinoid) from Baiyangdian Lake and Miyun Reservoir: Some implications for monitoring water environment

  Otolith is a typical biomineral carrier growing on insides of fish skull with prominent zoning structure formed by alternating layers of protein and calcium carbonate growing around the nucleus. Even though thermoluminescence (TL) analysis on biomineral has been widely used to measure the radiation exposure in the recent twenty years, the TL characteristics of the fish otolith have not yet been...

Author(s): Cao Ye, Li Shengrong, YAO Meijuan, Niu Huapeng, Luo Junyan, Du Fengqin, Gao Yonghua, Yang Liangfeng and Tong Jinggui

May 2012

The impacts of groundwater heat pumps on urban shallow groundwater quality in Shenyang China

  In order to assess the impacts of groundwater heat pumps on urban shallow groundwater quality in Shenyang China, the urban groundwater samples from pumping and recharging wells of groundwater heat pumps were collected during heating season in the months of November, September, January, February and March in 2007 and 2008. 17 groundwater quality parameters were analyzed using Chinese state or...

Author(s): Fengming Xi, Xinbei Wang, Yong Geng, Meiling Wang and Jing An

May 2012

Utilization of hydrogen gas production for electricity generation in fuel cell by Enterobacter aerogenes ADH 43 with many kinds of carbon sources in batch stirred tank reactor

  Enterobacter aerogenes ADH-43 is a hydrogen gas (H2) producing mutant bacterium and a facultative anaerobic microbe. This double mutant was obtained by classical mutagenetically treated in order to enhance H2 production. In addition, this mutant has ability to degrade molasses from sugar factory as well as other carbon sources. The main goals of this research were to use E....

Author(s): M. A. Rachman, Eniya L. D, Y. Liasari, M. M. Nasef, A. Ahmad and H. Saidi

May 2012

Respective effects of sodium and chloride ion on physiological property of Zymomonas mobilis 232B

  Respective effects of sodium and chloride ion on growth, cell morphological changes, membrane disorganization, ion homeostasis, exoenzyme activities and fermentation performance in Zymomonas mobilis232B cultures were presented. In batch cultures containing 0.15 M NaCl, Z. mobilis232B developed filaments, and growth and ethanol production were inhibited remarkably. Chloride ion (as NH4Cl) produced...

Author(s): Xinhui Wang,, Yuhong Huang,, Yang Fang, Yanling Jin, Guohua Zhang, Xiao feng Gao, and Hai Zhao,

May 2012

Effect of probiotics on microbial level in Azerbaijan native duck (Anas platyrhynchos) meat

  Probiotics are products of microbial cells that have useful effect on health and tranquility of human. According to several studies, valuable properties such as anti-carcinogenic, anti-mutagenic, increasing body immunity and resistance against entero-pathogens have been related to probiotics. Hence, the aim of this study was to specify the effect of probiotics usage in duck diet on microbial hazards of meat....

Author(s):    Javadi, A. and Ghazi Hashtroudi, H.   

May 2012

Purification of recombinant C-terminus polyhistidine tagged human calcitonin with cobalt-based and nickel-based affinity chromatography: A comparative study

  Human calcitonin (hCT) is a 32 amino acid peptide that participates in the regulation of calcium and phosphorus metabolism in human. It is used in clinics for thetreatment of diseases related to bone decalcification, such as Paget disease, osteoporosis imperfecta and parathyroid gland carcinoma. In this research, C-terminus polyhistidine tagged human recombinant calcitonin which was expressed byPichia...

Author(s): Ali Salehzadeh,Hamideh Ofoghi and Amir Arasteh

May 2012

Omental pedicle transposition and suture repair of peripheral nerve: A comparative study of functional, histological, morphometric and relative gastrocnemius muscle weight in rabbits

  The peripheral nervous system is able to regenerate after injury. Etiologies of injuries include penetrating injury, crush, traction, and ischemia compression. However, the presence of various nerve injury treatments such as coaptation and another technique to attain functional nerve regeneration are still inadequate. This study aimed to compare the effectiveness of omental pedicle transposition and epineural...

Author(s): Al-Timmemi, H. A., Ibrahim, R., Zuki, A. Z. and Azmi, T. I.

May 2012

Species richness and true diversity of cynipid galling-wasps community in oak forests of West-Azerbaijan Province (Iran)

  In this survey, the oak gall wasps (Hymenoptera: Cynipidae: Cynipini) were collected from oak forests of West-Azerbaijan Province in six sites, from April to October. Species richness, heterogeneity, evenness and true diversity were measured. Based on the result of this study, 37 of oak gall wasps species on oak trees (Quercus infectoria Oliv. and Quercus brantii Lindl.) were identified in...

Author(s): Abbas Hosseinzadeh

May 2012

Effects of exogenous hormones on plasma cortisol, sex steroid hormone and glucose levels in male and female grass carp, Ctenopharyngodon idellus, during the spawning induction

  Hormonal manipulation is a common spawning technique in aquaculture. In the present study, 15 male grass carp (Ctenopharyngodon idellus) with mean weight of 5 kg and 30 females with mean weight of 8 kg were used. Broodstock were injected with ovaprim (salmon gonadotropin- releasing hormone analogue in combination with domperidone) and human chronic gonadotropin (HCG) with a dosage of 4 mg for female/kg BW and...

Author(s): Seyed Ehsan Mousavi, and Mehdi Yousefian

May 2012

The effect of different levels of prebiotic on the length of fingerling rainbow trout

  This work was carried out to evaluate the effect of different levels (0.0, 0.5, 1, 1.5 and 2 kg per ton) of prebiotics (yeast culture concentrate derived from the outer cell wall ofSaccharomyces cervisiae) on the length trait of the fingerling rainbow trout. This experiment was conducted in split plot with complete randomize design (CRD) amid 500 rainbow trout (each 40 g). The length of fishes was...

Author(s): Abolfazl Ghorbani, Ramin Salamatdoustnobar, Seyaed Saied Ghaem Maghami and Vail Motallebi

May 2012

Effects of magnetic fields pretreatment of mungbean seeds on sprout yield and quality

  The aim of this investigation was to determine the effect of magnetic field pretreatment of mungbean seeds on the yield and quality of sprout. The sprout elongation, biomass and nutrition ingredients (for example, concentration of soluble sugar, protein, vitamin C, etc.) were measured to test this effect of magnetic field. The results show that magnetic field stimulation caused 25, 9 and 5.4% increment in...

Author(s): Yi-Ping Chen, Jun-Min He and Ran Li

May 2012

Study of thirty-one economically important traits in twenty silkworm Bombyx mori varieties

  The aim of this experiment was to study thirty-one economically important traits in twenty silkworm Bombyx mori breeds varieties. These varieties included 31, 1005, 113 (2029), 153 (Xihang-1), 5118 × 10133-3-3, F6 × 101, 1433-15, 1126 (111), 113-K, 1003-4, M2-6-18 (109), M2-6-22 (107), 151 (103 × M-1-1), Xihang 2/3, M-1-1 × 103, 7409, 107-K, 103, T5-M and 101433-1-4....

Author(s): Alireza Seidavi