African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Purification of recombinant C-terminus polyhistidine tagged human calcitonin with cobalt-based and nickel-based affinity chromatography: A comparative study

Ali Salehzadeh1*,Hamideh Ofoghi2 and Amir Arasteh1
  Department of Microbiology, Rasht Branch, Islamic Azad University, Rasht, Iran. 2Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran.
Email: [email protected]

  •  Accepted: 26 January 2012
  •  Published: 03 May 2012

Abstract

 

Human calcitonin (hCT) is a 32 amino acid peptide that participates in the regulation of calcium and phosphorus metabolism in human. It is used in clinics for thetreatment of diseases related to bone decalcification, such as Paget disease, osteoporosis imperfecta and parathyroid gland carcinoma. In this research, C-terminus polyhistidine tagged human recombinant calcitonin which was expressed byPichia pastoris strain KM71H was purified with cobalt-based IMAC resin and Nickel-based IMAC column. The result shows that the yield of purification was higher by cobalt-based IMAC resin in comparison with nickel-based IMAC column.

 

Key words: Human calcitonin, Pichia pastoris, cobalt-based IMAC resin, nickel-based IMAC column.

Abbreviation

CT, Calcitonin; hCT, human calcitonin; IMAC, immobilized metal ion affinity chromatography.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 11 No. 36
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES