African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12485

Table of Content: 5 July, 2017; 16(27)

July 2017

Silver nanoparticles biosynthesis and impregnation in cellulose acetate membrane for anti-yeast therapy

Silver nanoparticles (AgNPs) are nanoforms that express higher antimicrobial potential due to their shape and reduced size. The use of fungi to mediate the synthesis of AgNPs has increased the interest of scientists because of their rapid growth, large-scale cultivation and secretion of non-toxic molecules. The aim of this study was to synthesize AgNPs mediated by Aspergillus oryzae DPUA 1624 and evaluate the...

Author(s): Taciana A. Silva, Patrícia F. Andrade, Karen Segala, Larissa S. C. Silva, Larissa P. Silva, Silvia V. G. Nista, Lucia H. I. Mei, Nelson Durán, and Maria F. S. Teixeira

July 2017

Generation of gua1 deletion using polymerase chain reaction (PCR)-mediated gene disruption method in fission yeast, Schizosaccharomyces pombe

Gua1 gene in Schizosaccharomyces pombe encodes inosine 5′-monophosphate dehydrogenase (IMPDH), which catalyzes the first step in de novo biosynthesis of guanosine monophosphate (GMP). Knock-out cassette was constructed with polymerase chain reaction (PCR)-based gene targeting technique for deletion of gua1 gene in S. pombe and this knock-out cassette was transformed to S.s pombe wild type (972h-). Knock-out...

Author(s): Merve YILMAZER, Bedia PALABIYIK, Aysegul TOPAL SARIKAYA and Semian KARAER UZUNER

July 2017

Fourier transform infrared spectroscopy (FTIR) profiling of red pigment produced by Bacillus subtilis PD5

Pigments play an important role in physiological and molecular processes such as photosynthesis, survival to oxidative damage and resistance to ultra violet radiation in microorganisms. This study isolated and characterized pigment produced by Bacillus subtilis PD5 from soil sample of rice field. The pigment was extracted using three solvent system, namely, methanol, n-hexane and ethyl acetate. Fourier transform...

Author(s): Neha Trivedi, Shishir Tandon and Ashutosh Dubey

July 2017

Evaluation of antihyperglycemic, antiradical and acute oral toxicity activities of aqueous leaves extract of Moringa oleifera Lam (Moringaceae) from Benin in normal rats

Moringa oleifera is a commonly cultivated plant in the world. It has a high economic impact owing to its medicinal and nutritional values. Leaves of M. oleifera also contain various constituents that are useful for therapeutic purposes. The aim of this study is (i) to perform phytochemical screening, and to check antiradical and antihyperglycaemic activities of M. oleifera aqueous leaves extract (ii) to control the...

Author(s): LALEYE Obafèmi Arnauld Fernand, AZANDO Erick Virgile Bertrand,, OLOUNLADE Abiodoun Pascal,, TOHOUEGNON Théophile, LALEYE Anatole and AHISSOU Hyacinthe

July 2017

Molecular discrimination of Trypanosoma vivax and Trypanosoma congolense isolates of Nigerian origin

The present study was conducted to assess the molecular discrimination of field isolates of Trypanosoma congolense and Trypanosoma vivax of Nigeria origin in vitro (PCR) whether there will be genetic alteration of the parasites as the infections of the isolates in Yankasa sheep progresses from acute through relapses to chronic stages. A total of thirty Yankasa sheep were acquired from Kastina State in the northern part...

Author(s): C. I. Ogbaje, I. A. Lawal and J. K. P. Kwaga

July 2017

Evaluation of hypolipidemic activity of different extracts of Chomelia asiatica (Linn) in rat fed with atherogenic diet

In the present investigation, the hypolipidemic activity of different extracts of aerial parts of Chomelia asiatica (Linn) is shown. The evaluation dose of different extracts (petroleum ether, ethyl acetate and methanol) of C. asiatica was fixed at 200 mg/kg/day. Hypolipidemic activity was screened by inducing hyperlipidemia with the help of atherogenic diet (AD) in Wistar albino rats and plasma levels of different...

Author(s): Abdul Hameed Thayyil, Arumugam Kottai Muthu and Mohammed Ibrahim

July 2017

Microbial diversity in leaves, trunk and rhizosphere of coconut palms (Cocos nucifera L.) associated with the coconut lethal yellowing phytoplasma in Grand-Lahou, Côte d’Ivoire

The diversity of culturable bacterial and fungal communities was assessed from leaves, trunk and rhizosphere of coconut palms infected and non-infected by the Côte d’Ivoire lethal yellowing (CILY) phytoplasma. Bacterial and fungal microbes were isolated from leaves, trunk and rhizosphere samples collected from two villages of Grand-Lahou, Braffedon and Yaokro, by using a high-throughput...

Author(s): Nadia P. Morales-Lizcano, Ahmed Hasan, Henry S. To, Tacra T. Lekadou, Julia Copeland, Pauline Wang, Hortense A. Diallo, Jean L. Konan Konan, Keiko Yoshioka, Wolfgang Moeder, James Scott and Yaima Arocha Rosete

July 2017

Generating virus-free cassava plants by in vitro propagation with chemical and heat treatment

Cassava production in eastern and Central Africa is severely threatened by the current epidemic of cassava brown streak disease (CBSD). The disease is caused by cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) of the genus Ipomovirus, family Potyviridae. Using virus-free planting material is one effective way to reduce CBSD yield losses in farmer fields. The effects of chemical and heat...

Author(s): Mohammed I. U., Ghosh S. and Maruthi M. N.