African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Table of Content: 7 June 2010; 9(23)

June 2010

Pollen-pistil compatibility relationships in some Iranian almond (Prunus dulcis, Batch) genotypes as revealed by PCR analysis

  The identification of pollen-pistil compatibility relationships among almond cultivars and genotypes is very important for breeders and growers. In the present study, PCR based technique was used to identify S-alleles in 10 late blooming almond genotypes. In total, 19 alleles were amplified by five primer pairs in the studied genotypes. The size of bands ranged between 480 - 2000 bp. Seven S-alleles were...

Author(s): Yavar Sharafi, Jafar Hajilou, Seyed AbolGhasem Mohammadi, Mohammad Reza Dadpour and Sadollah Eskandari

June 2010

Molecular cloning and functional analysis of the gene encoding geranylgeranyl diphosphate synthase from Jatropha curcas

 Geranylgeranyl diphosphate (GGPP) synthase (GGPPS, EC: 2.5.1.29) catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic diphosphates to yield (all-E)-GGPP. GGPP is one of the key precursors in the biosynthesis of biologically significant isoprenoid compounds. Here we report for the first time the cloning of a full-length cDNA encoding GGPPS (Jc-GGPPS) from Jatropha curcas L....

Author(s): Juan Lin, YuanJie Jin, Xin Zhou and JingYa Wang

June 2010

Amplified fragment length polymorphism (AFLP) and genealogy analysis of the introgressed lines from Gossypium hirsutum × Gossypium barbadense varieties

  In this study, amplified fragment length polymorphism (AFLP) technique was used to analyze the genealogical relationship of 7 introgressed cotton varieties from the hybridization of upland (Gossypium hirsutum L) and sea-island cottons (Gossypium barbadense L). Ten pairs of primer combinations with high polymorphism and resolution were selected from 64 primers. These 10 primer combinations resulted...

Author(s): Zhao-hui Liu, Yong Zhang, Jinhua Peng, Kejun Deng, Shunwen Dongand Zhenglong Ren,

June 2010

Phylogenetic relationships of eleven Kobresia accessions from the Tibetan plateau

 In the past, identification of the genus Kobresia was mostly dependent on morphological characteristics. This study used random amplified polymorphic DNA (RAPD), sequences of nrDNA ITS, cpDNA trnT-L-F spacer and cpDNA ndhF to assess the phylogenetic relationships among the accessions of Kobresia plants collected from the Tibetan plateau. In the dendrograms, Kobresia macrentha (L)...

Author(s): Peizhi Yang, Hongmei Zheng, Steven Larson, Yanjun Miao and Tianming Hu

June 2010

Molecular marker analysis of heading date Hd1 locus in Egyptian rice varieties

  Nine molecular markers derived from the heading date QTL Hd1 DNA sequence for cultivated rice were used to study the heading date allelic diversity of the cultivated Egyptian rice varieties. The results showed that among the nine simple sequence repeats (SSR) and sequence tagged-sites (STS) markers used, one SSR marker, Hd1AGC, amplified three polymerase chain reaction (PCR)...

Author(s): R. M. Gaafar

June 2010

Growth and yield response of wheat to EM (effective microorganisms) and parthenium green manure

  Parthenium weed (Parthenium hysterophorus L.) is rapidly spreading in many countries around the world and has become a serious threat to nature as well as managed ecosystems. The present pot experiment was conducted in 2005 -2006 to study the effect of parthenium green manure (1, 2, 3 and 4% on fresh weight bases) and EM (effective microorganisms), a biofertilizer, on the growth...

Author(s): Arshad Javaid and M.B.M. Shah

June 2010

The leaf size-twig size spectrum in evergreen broad-leaved forest of subtropical China

 Plant twig characters for 82 woody species including individual leaf and total leaf mass, stem mass, individual leaf area, total leaf area and stem cross-sectional area were investigated at the twig level in different altitudes and life forms on Meihuashan Mountain in Southeastern China. The results showed that twig cross-sectional area of plant twigs were found to allometrically scale to individual leaf area and...

Author(s): Zhiguo Liu, Yongli Cai, Kai Li, and Jiguang Guo

June 2010

The effects of sheep manure application time and rates on yield and botanical composition of secondary succession rangeland

  This experimental study was conducted in Samsun/Turkey on a secondary succession rangeland during 2006 - 2008. Botanical composition of the experimental area consists of 28% legumes, 64% grasses, and 8% of other species. Target rates of sheep manure were 0, 25.0, 50.0, 75.0, and 100 kg total N ha–1, based on the manure analyses results (according to N rates in sheep manure). The sheep manure was...

Author(s): Hanife Mut, Ilknur Ayan, Ugur Basaran, Ozlem Onal-Asci and Zeki Acar

June 2010

Degradation of di-, tri-, tetra-, and pentachlorophenol mixtures in an aerobic biofilter

  Chlorophenols are highly toxic xenobiotic compounds, normally found in the effluents of many industries. Due to the high toxicity of these compounds, it is difficult to treat effluent streams containing high concentrations of chlorophenols. The aim of this work is to demonstrate the capabilities of an aerobic submerged filter previously characterized in the degradation of phenol and chlorophenols in the...

Author(s): Luis G. Torres, Miguel Hernández, Yolanda Pica, Verónica Albiter and Erick R. Bandala

June 2010

Dissolution kinetics and mechanism of pandermite in acetic acid solutions

 In this study, the dissolution kinetics and mechanism of pandermite mineral was investigated using a batch reactor employing the parameters of particle size, acid concentration, solid/liquid ratio, stirring speed and reaction temperature. From experimental data, it was determined that the conversion rate of pandermite to boric acid was increased with decreasing particle size, solid/ liquid ratio and increasing...

Author(s):    Aycan Gur, Firat Caglayan, Adnan Yildiz and Ahmet Selcuk   

June 2010

Purification and characterization of a thermostable glucoamylase produced by Aspergillus flavus HBF34

  Glucoamylase (GA) from Aspergillus flavus HBF34 strain was partially purified 120 folds using starch affinity chromatography. Two isoenzymes (GA1 and GA2) were identified by polyacrylamide gel electrophoresis (PAGE) zymography. Sodium dodecyl sulfate (SDS)-PAGE analysis revealed that one of the enzymes consist of one subunit and the other, two subunits. The optimum pH of the purified GA was 6.0 and...

Author(s): Öznur Koç and Kubilay Metin

June 2010

Mixture of residual fish hydrolysate and fish extract hydrolysate to activate Bacillus licheniformis 6346

 Microbes are first activated in appropriate media before cultivation/fermentation.Bacillus licheniformis 6346 activated in nutrient broth-starch was inoculated to a locally formulated solid medium (paddy husk, rice flour, (NH4)2SO4, cuttle fish powder, murate potash, table salt, triple super phosphate, sesame oil, coconut oil and tap water) and the highest α-amylase activity was obtained on day 5 (1075...

Author(s): Vasanthy Arasaratnam and Kulasingam Thayaananthan

June 2010

Enhancement of the performance of covalently immobilized lipase using alcohol quenching technology

  In order to modulate the microenvironment for enzyme covalently attached on support and improve the covalent immobilization of lipase, alcohol molecules were used to quench the excessive activated functional group on support surface. Effects of kind and content of alcohol molecules on the relative activity of the immobilized enzyme and the characteristics of the immobilized enzyme were examined carefully. The...

Author(s): Anming Wang, Haifeng Li, Zhenming Chen, Xiaolin Pei, Zhaowu Zeng, Lifeng Huang, Xiaopu Yin and Tian Xie

June 2010

Stimulation of artemisinin biosynthesis in Artemisia annua hairy roots by oligogalacturonides

  The different fractions of oligogalacturonides (OGA) from polygalacturonic acid by pectinase hydrolysate have been partially purified using column chromatography of Sephadex G-10. The isolated fraction OGA2 (degree of polymerization, DP = 4.57) was found to stimulate the accumulation of artemisinin in Artemisia annua hairy roots. When hairy roots of 16-day old cultures were exposed to the OGA...

Author(s): Ben Zhang, Ting Zou, Yan Hua Lu and Jian Wen Wang,

June 2010

Detection of hepatocellular carcinoma with multi-slice spiral CT by using double-arterial phase and portal venous phase enhanced scanning: Effect of iodine concentration of contrast material

 The purpose of the study is to evaluate the effect of iodine concentration of contrast material on detection of hepatocellular carcinoma with multi-slice spiral computed tomography (CT) by using double-arterial phase and portal venous phase enhanced scanning. Ninety-four (94) patients with hepatocellular carcinoma (HCC) were examined by hepatic plain CT and contrast-enhanced CT including early arterial phase...

Author(s): Jian-shou Zhou, Yi Huan, Meng-qi Wei and Xin-qing Jiang

June 2010

Quantitative analysis of hepatocellular adenomas with triphasic contrast enhanced multislice spiral computed tomography (MSCT)

  The purpose of this study is to evaluate the enhancement characteristics of hepatocellular adenomas with triphasic contrast enhanced computed tomography (CT) by quantitative analysis of 19 lesions in 11 patients. There were significant differences between the attenuation values of hepatocellar adenomas and liver in unenhanced, arterial and delayed phases, but there were no significant differences in...

Author(s): Jian-shou Zhou, Yi Huan and Meng-qi Wei

June 2010

Inhibition of inflammatory factors by parthenolide in human renal mesangial cells under hyperglycemic condition

 The aim of this study was to investigate the anti-inflammatory effects of parthenolide (PTN) in human renal mesangial cells (HRMCs) under high ambient glucose conditions. First we determined the non-cytotoxic concentration of PTN in HRMCs by performing the MTS assay. Enzyme-linked immunosorbent (ELISA) analysis was performed to determine the expressions of interleukin (IL)-1β, IL-18, tumor-necrosis...

Author(s): Wen-Juan Wang, Fang Wu, Yun Qian, Lin-Fang Cheng, Xue-Ting Shao, Xiang-Min Tong and Hong Li

June 2010

Comparison of a modified peptone water transport medium with two commercially available transport media for the recovery of aerobes from swab specimens

  A laboratory modified peptone water medium was evaluated alongside Stuart and Amies media for their relative suitability as transport media for aerobic bacteria isolated from wound specimens obtained from Central Hospital, Benin City, Nigeria. The survival rates of isolates from the three media were assessed quantitatively for a period of seven successive days, using the pour-plate method for making viable...

Author(s): J. O. Isibor and P. U. Amadi

June 2010

Quantificational analysis of NPT-II protein from genetically modified Vitis vinifera L.

  Widely distributed inhibitors in grapevine extracts make it difficult to improve analytical procedures for protein detection. In this study, acidity in grapevine extracts was one of the major factors inhibiting the detection of neomycin phosphotransferase II via enzyme-linked immunosorbent assay. Leaf and berry extracts with low pH (3.0 – 4.0) strongly inhibited NPT-II detection, while root and xylem...

Author(s): Seok Hyun Eom and Bruce I. Reisch

June 2010

Anti-malarial activity of ethanolic leaf extract of Piliostigma thonningii Schum. (Caesalpiniacea) in mice infected with Plasmodium berghei berghei

 Piliostigma thonningii Schum. (Caesalpiniacea) is used traditionally in the management of fever, cough, wounds and various ulceration. Oral acute toxicity of the ethanolic leaf extract of Piliostigma thorningii was evaluated in mice using modified Lorke’s method. The ethanolic leaf extract was evaluated for in vivo anti plasmodial activity against chloroquine sensitive strain...

Author(s): A. A. Madara, J. A. Ajayi, O. A. Salawu and A. Y. Tijani