African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12480

Full Length Research Paper

Amplified fragment length polymorphism (AFLP) and genealogy analysis of the introgressed lines from Gossypium hirsutum × Gossypium barbadense varieties

Zhao-hui Liu1,2, Yong Zhang1,3*, Jinhua Peng1, Kejun Deng1, Shunwen Dong2and Zhenglong Ren1,3*
  1School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chendu, 610054, P. R. China. 2Industrial Crop Breeding and Cultivation Institute, Sichuan Academy of Agriculture Sciences, Jianyang 641400, P. R. China. 3Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Ya’an, 625014, P. R. China.
Email: [email protected], [email protected]

  •  Accepted: 21 May 2010
  •  Published: 07 June 2010

Abstract

 

In this study, amplified fragment length polymorphism (AFLP) technique was used to analyze the genealogical relationship of 7 introgressed cotton varieties from the hybridization of upland (Gossypium hirsutum L) and sea-island cottons (Gossypium barbadense L). Ten pairs of primer combinations with high polymorphism and resolution were selected from 64 primers. These 10 primer combinations resulted in a total of 480 bands, of which 374 bands (77.9%) were polymorphic and 51 bands (10.6%) were specific. Cluster analyses showed that 7 varieties of cottons were divided into two groups. Our results suggested that interspecific hybridization is feasible to broaden germplasm of upland cotton and AFLP technique could be applied for the identification of variety purity and genealogical relationship.

 

Key words: Cotton, introgressed line, AFLP, genealogical analysis.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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