June 2016
An improved plating assay for determination of phage titer
Phage is a virus that is parasitic on bacteria. It is very important to determine the titer of test sample in the study of phage. In this study, an improved plating assay was developed for detection of the number of recombinant phage Cap-T7 present in a test solution at a certain dilution point by counting the plaque forming units. The data demonstrated that the improved plating assay is fast, useful, and convenient for...
June 2016
Improving the optimum yield and growth of Chlamydomonas reinhardtii CC125 and CW15 using various carbon sources and growth regimes
Chlamydomonas reinhardtii CC125 (wild type) and CW15 (cell wall mutants) were feed up on solid and liquid Tris phosphate (TP) media with various concentrations of acetate, glycerol(10-100 mM) or methanol (0.01-718 mM) and cultivated under phototrophic, mixotrophic and heterotrophic conditions. Use of 10 and 35 mM acetate and 10 and 50 mM glycerol under constant 38 µE m−2 s−1 light illumination...
June 2016
Variation in levels and removal efficiency of heavy and trace metals from wastewater treatment plant effluents in Cape Town and Stellenbosch, South Africa
This study focused on one year monitoring campaign to monitor the occurrence and removal of Endocrine Disruptive Metals (EDMs) and trace metals from selected wastewater treatment plants (WWTPs) in Stellenbosch and Cape Town. Composite water samples were collected from the WWTPs from January 2010 to December 2010 on a quarterly basis and concentrations determined using inductively coupled plasma-mass spectrometry...
June 2016
Genetic diversity studies on selected rice varieties grown in Africa based on aroma, cooking and eating quality
Rice grain quality is an important factor that has a great influence on its market value and consumer acceptance. It is determined by three parameters controlling the cooking and eating qualities of rice (amylose content, gelatinization temperature and gel consistency) and by the aroma, which becomes a criterion increasingly preferred by consumers. Molecular characterization of specific genomic regions of rice genotypes...
June 2016
Genetic diversity and population structure among sorghum (Sorghum bicolor, L.) germplasm collections from Western Ethiopia
The Western Ethiopian region harbors a unique set of sorghum germplasm adapted to conditions not conventional to sorghums grown in other parts of the world. Accessions from the region possess unique resistance to multiple leaf and grain diseases. This study is aimed at exploring the extent of genetic variation and population structure among accessions of this region. A total of 123 accessions comprising 111 from Western...
June 2016
Optimal concentration of selective agents for inhibiting in vitro growth of Urochloa brizantha embryogenic calli
The ability to distinguish transgenic cells of untransformed cell mass is a key step for the production of transgenic plants. Thus, the use of selection marker genes for identification of genetically modified plants is necessary. The aim of this study was to determine the optimal concentration of four selective agents (kanamycin, hygromycin, phosphinothricin and mannose) to inhibit in vitro growth of Urochloa brizantha...
June 2016
Bioactivity of mangrove humic materials on Rizophora mangle and Laguncularia racemosa seedlings, Brazil
The mangrove sediments are rich in organic matter and humic substances, responsible for important functions such as reducing the toxicity of heavy metals, nutrient stabilization, serving as drain to atmospheric carbon and increasing the plant growth. In this study, we observed the effect of humic substances, humic acids and fulvic acids isolated from sediments of a mangrove forest, sampled from the Municipal Ecological...
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