African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12474

Table of Content: April 2004; 3(4)

April 2004

Comparative analysis and relationships of six important crop species chloroplast genomes using whole genome web-based informatics tools

Using informatics tools to compare important species is now feasible as structural genomics continue in importance and establishment of structure-function relationships become a common way of comparative analysis. Currently, many of the technical issues involved in sequencing complete genomes have been solved. The smallness in size of chloroplast genomes facilitates being used for the discovery of disease resistance...

Author(s): Beatrice Kilel

April 2004

Analysis of Tunisian date palm germplasm using simple sequence repeat primers

Inter Simple Sequence Repeat (ISSR) markers involve polymerase chain reaction (PCR) amplification of DNA using a single primer composed of a microsatellite sequence. ISSR technology rapidly reveals high polymorphic fingerprints and determines genetic diversity. We used genetic markers generated from selected ISSR primers toassess genetic diversity among a set of Tunisian date palm varieties. Seven primers...

Author(s): Zehdi S., Sakka H., Rhouma A, Ould Mohamed Salem A. Marrakchi M.and Trifi M.

April 2004

Analysis of genetic diversity in bambara groundnut [Vigna subterranea (L.) Verdc] landraces using amplified fragment length polymorphism (AFLP) markers

Amplified fragment length polymorphism (AFLP) was used to assess genetic diversity among 100 selected bambara groundnut [Vigna subterranea (L.) Verdc] landraces from a diverse geographic area of Tanzania. Eleven informative AFLP primer combinations generated a total of 49 scorable polymorphic amplification fragments across the bambara groundnut accessions. Genetic distances between all accessions based on...

Author(s): Wazael H. Ntundu, , Inga C. Bach, Jørgen L. Christiansen and Sven B. Andersen

April 2004

Direct genetic transformation of Hibiscus sabdariffa L.

Transgenic Hibiscus sabdariffa plants have been produced by a tissue culture independent method using Agrobacterium tumefasciens transformation procedure. Embryo axes of mature seeds with one cotyledon excised were infected by immersion in a suspension of Agrobacterium LBA 4404 strain culture that carries pBal plasmid with β-glucuronidase p35SGUSINT and plant selectable marker Neomycin...

Author(s): Yaye Kène Gassama-Dia, Djibril Sané and Mansor Ndoye

April 2004

A morphometric analysis of the genus Ficus Linn. (moraceae)

Foliar parameters of Ficus in Nigeria were subjected to quantitative analysis. The morphometric analysis is based on ten quantitative parameters of the leaves of species. Principal compound analysis produced six groups whose characters are described. Highly significant positive correlation exists between leaf length and leaf width, leaf length and lamina length, leaf length and petiole length, lamina length...

Author(s): Mubo, A. Sonibare, Adeniyi, A. Jayeola and Adeyemi Egunyomi

April 2004

Nutrients requirements in biological industrial wastewater treatment

Wastewaters from olive mills and pulp and paper mill industries in Jordan have been characterized and treated using laboratory scale anaerobic and aerobic sequencing batch reactors, respectively. Nutrient requirements for these two industrial wastewaters were found to be less than what is usually reported in the literature for C:N:P ratio of 100:5:1 for aerobic treatment and 250:5:1 for anaerobic treatment. This was...

Author(s): Bashaar Y. Ammary

April 2004

Potential of cassava flour as a gelling agent in media for plant tissue cultures

The potential of a tissue culture medium gelled with cassava flour to support shoot proliferation of stem nodal sections of Uapaca kirkiana and Faidherbia albida was studied. A two factorial experiment was conducted with the first factor as type of gelling agent (80 g/L cassava flour; 80 g/L cassava flour mixed with 3.5 g/L agar; and 7 g/L agar) while the second factor was two plant species....

Author(s): Moses F. A. Maliro, and Grace Lameck

April 2004

A Study on the use of carrot juice in the tissue culture of Daucus carota

Effects of using carrot juice in the in vitro growth of Daucus carota were investigated. Callus was the only form of growth obtained after five weeks in culture. The increase in fresh weight, dry weight and moisture content of the explants with increasing concentration of carrot juice in the medium was much lower when compared to either the cytokinin benzyladenine (BA) and/or the auxin picloram....

Author(s): D. Puchooa and R. Ramburn

April 2004

A simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.)

A simple, rapid and efficient method for isolating genomic DNA from lychee (Litchi chinensis Sonn.) was developed. This modified CTAB protocol include the use of 2 M NaCl, PVP, 5% mercaptoethanol and 80% ethanol in the extraction as well as reducing the centrifugation times during the separation and precipitation of the DNA. Isolated genomic DNA showed high purity and high quantity following restriction digestion...

Author(s): D. Puchooa