African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Short Communication

A simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.)

D. Puchooa
Faculty of Agriculture, University of Mauritius, Réduit, Mauritius. 
Email: [email protected]

  •  Accepted: 16 March 2004
  •  Published: 30 April 2004

Abstract

A simple, rapid and efficient method for isolating genomic DNA from lychee (Litchi chinensis Sonn.) was developed. This modified CTAB protocol include the use of 2 M NaCl, PVP, 5% mercaptoethanol and 80% ethanol in the extraction as well as reducing the centrifugation times during the separation and precipitation of the DNA. Isolated genomic DNA showed high purity and high quantity following restriction digestion analysis.

 

Key words: Litchi chinensis, genomic DNA isolation.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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