African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: April 2005; 4(4)

April 2005

Molecular approaches to detect and study the organisms causing bovine tick borne diseases: babesiosis and anaplasmosis

  This review will summarise the molecular approaches used to detect and analyse the genomes of Babesia bovis, B. bigemina and Anaplasma marginale which causebovine babesiosis and anaplasmosis. These tick borne diseases are widely distributed in Africa, Asia, Australia, and Central and South America and for example, have been estimated to have an economic impact of US$15.9, $6.9, $6.2,...

Author(s): Ala Lew and Wayne Jorgensen

April 2005

Herbal remedies in animal parasitic diseases in Nigeria: a review

This review of literature elucidates previous and current status of herbal remedies in animal parasitic diseases in Nigeria. It provides background information on the rationale behind ethnoveterinary research in general especially as it relates to the developing nations where cost of drugs majorly limit the full use of modern medicine. It presents in quantifiable terms the degree of efficacy of whole or plant parts and...

Author(s): FAJIMI, A. K.  and TAIWO, A. A.

April 2005

Contrasting genetic diversity among Oryza longistaminata (A. Chev et Roehr) populations from different geographic origins using AFLP

  Molecular markers have been used extensively in studying genetic diversity, genetic relationships and germplasm management. However, the understanding of between and within population genetic variation and how it is partitioned on the basis of geographic origin is crucial as this helps to improve sampling efficiency. The objective of this study was therefore to assess the intra-specific diversity in Oryza...

Author(s): D. K. Kiambi, H. J. Newbury, B. V. Ford-Lloyd and I. Dawson

April 2005

Genetic variation of Japanese loach inferred from restriction fragment length polymorphism analysis of mitochondrial DNA

  Genetic analysis using restriction fragment length polymorphisms (RFLPs) of D-loop region in the mitochondrial DNA including the part of 12s RNA and cytochrome b genes was made to clarify genetic variations and relations and relationships among eleven populations of Japanese loach Misgurnus anguillicaudatus. Ten haplotypes were detected using seven restriction enzymes...

Author(s): Md Mukhlesur Rahman Khan,, Katsutoshi Arai, Kenji Kuroda, Tetsuya Umino, and Heisuke Nakagawa

April 2005

The effect of media, oil type and rate on the mycelia wet and dry weights of Lentinus squarrosulus (Mont.) singer and psathyrella patroumbonata pegler in submerged liquid culture

  Lentinus squarrosulus and Psathyrella atroumbonata, two edible indigenous mushroom species, were cultured in four different submerged liquid media supplemented with coconut, cotton, groundnut, butterfat, palm kernel and palm oil at 5 different rates. The differences in the means due to the different media types were highly significant (p<0.01) for the mean mycelia wet and dry weights of bothL....

Author(s): Nwanze, P. I., Khan, A. U., Ameh, J. B. and Umoh, V. J.

April 2005

Contribution of arbuscular mycorrhizal fungus to red kidney and wheat plants tolerance grown in heavy metal-polluted soil

  The researcher had investigated the role of arbuscular mycorrhizal fungal inoculation in red kidney and wheat in heavy metals tolerance in soil artificially contaminated with high concentrations of zinc, copper, lead and cadmium. Metals accumulated by mycorrhizal wheat plants were mostly distributed in root tissues, suggesting that an exclusion strategy for metal tolerance widely exists in them. Mycorrhizal...

Author(s): Gamal  Hassan Rabie

April 2005

Toward a molecular evaluation of grain quality using glutenin subunits in Triticum carthlicum

  The grain quality of tetraploid wheat, Triticum carthlicum was analyzed by examining seed storage glutenin (HMW) markers at Glu-A1, Glu-B1 and Glu-D1 loci by SDS-PAGE method, as well as confirmation by Zeleny and Farinograph tests. 15 populations were collected from Iran, some areas of the Middle East and the North of Africa with this assumption that this species has originated from the Middle East...

Author(s): Ardashir Kharabian Masouleh

April 2005

Response of sugarcane (Saccharum sp.) varieties to embryogenic callus induction and in vitro salt stress

  Response of three varieties of sugarcane (Saccharum sp.) to callus induction, embryogenic callus production and in vitro salt tolerance was studied. For callus induction and embryogenic callus production, leaf bases segments were subjected to in vitro culture on Murashige and Skoog (MS) medium supplemented with 3 mg l-1 2,4 Dichlorophenoxyacetic acid for 4 weeks. To evaluate salt...

Author(s): C. H. Gandonou, J. Abrini and N. Skali Senhaji

April 2005

Influence of water extract of Mexican sunflower (Tithonia diversifolia) on growth of cowpea (Vigna unguiculata)

  Laboratory analysis of the water extract of Tithonia diversifolia shoot and root on infra-red spectrometer to determine the phytotoxic potential and greenhouse experiments to evaluate its phytotoxic effect and growth promoting attribute on cowpea (Vigna unguiculata L.) were carried out. T. diversifolia contained some compounds having functional groups such as alcohols, olefins,...

Author(s): Taiwo, L. B. and Makinde, J. O.

April 2005

Pattern of rural-urban acquisition of pfcrt T76 allele among Nigerian children with acute uncomplicated Plasmodium falciparum malaria

Malaria caused by Plasmodium falciparum remains a public health problem in Nigerian children with treatment complicated by expansion of chloroquine resistant strains known to harbour a common K76T point mutation in their pfcrt alleles. Here, we report the outcome of a 2 – year (March 2000 – February 2002) molecular surveillance for pfcrtT76 in children aged 6 months – 13 years with acute...

Author(s): Olukosi, Y.A; Iwalokun, B.A; Magbagbeola, E.O; Akinwande, O; Adewole, TA; Agomo, PU.

April 2005

Detection of microfilariae with counting chamber technique in some Nigerian rural communities

The conventional stained thick smear technique (TS) has innate possibility of underestimation of microfilariae (mf) in microfilaraemic subjects. The usability of counting chamber (CC) technique in the detection of mf was determined in three Nigerian rural communities where Loa loa and Mansonella perstans are endemic. Blood samples were collected by finger-pricking method from 612 subjects (334 males, 278...

Author(s): Agbolade, O. M., & Akinboye, D. O. 

April 2005

Antimicrobial activity of the essential oil of Cestrum diurnum (L.) (Solanales: Solanaceae)

Cestrum diurnum (Solanaceae: Solanales) is a single or multistemmed shrub that is also known as Day Jasmine. The essential oil of the mature leaves of C. diurnumwas analyzed by GLC and GLC-MS and altogether 14 components were detected. The main constituents were palmitic acid (27.62%), stearic acid (4.62%) and oleic acid (3.06%). The essential oil of mature leaves of C. diurnum were evaluated for...

Author(s): Bhattacharjee I., Ghosh A. and Chandra G.