African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

The effect of media, oil type and rate on the mycelia wet and dry weights of Lentinus squarrosulus (Mont.) singer and psathyrella patroumbonata pegler in submerged liquid culture

Nwanze, P. I.1*, Khan, A. U.2, Ameh, J. B.3 and Umoh, V. J.4
  1Department Of Biological Sciences,College Of Natural And Applied Sciences, Igbinedion University,Okada, P.M.B. 0006,Edo State, Nigeria. 2Department Of Biological Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria. 3Department Of Microbiology, Ahmadu Bello University, Zaria. Kaduna State, Nigeria. 4Department Of Microbiology, Ahmadu Bello University, Zaria. Kaduna State, Nigeria.
Email: [email protected]

  •  Accepted: 28 February 2005
  •  Published: 30 April 2005

Abstract

 

Lentinus squarrosulus and Psathyrella atroumbonata, two edible indigenous mushroom species, were cultured in four different submerged liquid media supplemented with coconut, cotton, groundnut, butterfat, palm kernel and palm oil at 5 different rates. The differences in the means due to the different media types were highly significant (p<0.01) for the mean mycelia wet and dry weights of bothL. squarrosulus and P. atroumbonata. SLCM3, which contained twice the glucose level present in each of the other three liquid culture media, induced the heaviest mean wet and dry mycelia weights of both mushroom species. Oil type had a highly significant (p<0.01) effect on the wet and dry mycelia weights of L. squarrosulus as well as the mycelia wet weight of P. atroumbonata. Butterfat induced the heaviest weights. In addition, oil rate also had a significant effect (p<0.01) on the mean mycelia wet and dry weights of both mushroom species. The oil rates of 0.003, 0.005 and 0.007 ml/ml produced comparable optimum mean mycelia wet and dry weights of the mushrooms.

 

Key words: Lentinus squarrosulusPsathyrella atroumbonata, supplemented media, submerged liquid media, oil type and rate.